{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T01:41:57Z","timestamp":1791855717035,"version":"4.3.4"},"reference-count":65,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2022,7,26]],"date-time":"2022-07-26T00:00:00Z","timestamp":1658793600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\"><jats:title>Background<\/jats:title><jats:p>Coliform bacteria are major causative agents of bovine mastitis, a disease that has devastating effect on dairy animal health and milk production. This cross-sectional study, carried out in the North West region of Cameroon, sought to determine the prevalence of bovine mastitis, coliforms associated with bovine mastitis, risk factors for infection and the antibiotic resistance pattern of coliform bacterial isolates.<\/jats:p><\/jats:sec><jats:sec id=\"sec002\"><jats:title>Materials and methods<\/jats:title><jats:p>A total of 1608 udder quarters were sampled from 411 cows using a questionnaire, clinical examination, California Mastitis Test and milk culture. Primary isolation of coliform bacteria was done on MacConkey agar while identification of coliforms employed Gram-staining and biochemical testing. Each coliform bacterial isolate was challenged with 11 antibiotics using the Kirby-Bauer disc diffusion method.<\/jats:p><\/jats:sec><jats:sec id=\"sec003\"><jats:title>Results<\/jats:title><jats:p>The prevalence of mastitis was 53.0% (218\/411) and 33.1% (532\/1608) at the cow- and quarter-levels respectively. Overall, 21.9% (90\/411) cows and 8.2% (132\/1608) udder quarters showed coliform mastitis.<jats:italic>Escherichia coli<\/jats:italic>was isolated in 7.0% of mastitis milk, and other coliforms isolated were<jats:italic>Enterobacter cloacae<\/jats:italic>(12.6%),<jats:italic>Klebsiella pneumoniae<\/jats:italic>(2.4%),<jats:italic>Enterobacter sakazakii<\/jats:italic>(1.1%),<jats:italic>Klebsiella oxytoca<\/jats:italic>(0.8%),<jats:italic>Citrobacter freudii<\/jats:italic>(0.4%),<jats:italic>Serratia ficaria<\/jats:italic>(0.4%) and<jats:italic>Serratia liquefaciens<\/jats:italic>(0.2%). Lactation stage, breed, history of mastitis and moist\/muddy faeces contaminated environment were significantly associated (<jats:italic>P<\/jats:italic>-value &lt; 0.05) with coliform mastitis. Coliform isolates (99.0%; 203\/205) were resistant to at least one antibiotic tested. Amoxicillin had the highest resistance (88.8%) while norfloxacin had the least resistance (3.4%). Multidrug resistance was exhibited by 52.7% (108\/205) of the isolates in a proportion of 27.8%<jats:italic>Enterobacter cloacae<\/jats:italic>, 10.7%<jats:italic>E<\/jats:italic>.<jats:italic>coli<\/jats:italic>, 6.3%<jats:italic>Klebsiella pneumoniae<\/jats:italic>, 2.9%<jats:italic>Enterobacter sakazakii<\/jats:italic>, 2.0%<jats:italic>Klebsiella oxytoca<\/jats:italic>, 1.0%<jats:italic>Citrobacter freundii<\/jats:italic>, 1.0%<jats:italic>Serratia ficaria<\/jats:italic>, 0.5%<jats:italic>Serratia liquefaciens<\/jats:italic>and 0.5%<jats:italic>Serratia odorifera<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec id=\"sec004\"><jats:title>Conclusion<\/jats:title><jats:p>Results indicate a need to educate these dairy farmers about mastitis (particularly subclinical), proper hygiene methods in milking and the public health implications of consuming contaminated raw milk.<\/jats:p><\/jats:sec>","DOI":"10.1371\/journal.pone.0268247","type":"journal-article","created":{"date-parts":[[2022,7,26]],"date-time":"2022-07-26T17:34:47Z","timestamp":1658856887000},"page":"e0268247","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":32,"title":["Prevalence and risk factors of coliform-associated mastitis and antibiotic resistance of coliforms from lactating dairy cows in North West Cameroon"],"prefix":"10.1371","volume":"17","author":[{"given":"Ursula Anneh","family":"Abegewi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2497-4689","authenticated-orcid":true,"given":"Seraphine Nkie","family":"Esemu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland 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Dalanezi","year":"2020","journal-title":"J Dairy Sci"},{"issue":"1\u20132","key":"pone.0268247.ref003","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0378-4320(03)00133-7","article-title":"Effect of timing of first clinical mastitis occurrence on lactational and reproductive performance of Holstein dairy cows","volume":"80","author":"JE Santos","year":"2004","journal-title":"Anim Reprod Sci"},{"issue":"1","key":"pone.0268247.ref004","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1080\/01652176.2007.9695224","article-title":"Economic effects of bovine mastitis and mastitis management: A review.","volume":"29","author":"T Halasa","year":"2007","journal-title":"Vet Q"},{"issue":"4","key":"pone.0268247.ref005","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/s10911-011-9231-3","article-title":"Mastitis and its impact on structure and function in the ruminant mammary gland.","volume":"16","author":"RM Akers","year":"2011","journal-title":"J Mammary Gland Biol Neoplasia"},{"issue":"11","key":"pone.0268247.ref006","doi-asserted-by":"crossref","first-page":"1582","DOI":"10.2460\/javma.2003.222.1582","article-title":"Antimicrobial resistance of bacteria isolated from dairy cow milk samples submitted for bacterial culture: 8,905 samples (1994\u20132001).","volume":"222","author":"A Makovec","year":"2003","journal-title":"J Am Vet Med Assoc"},{"key":"pone.0268247.ref007","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1136\/vr.160.8.253","article-title":"Survey of the incidence and aetiology of mastitis on dairy farms in England and Wales","volume":"160","author":"AJ Bradley","year":"2007","journal-title":"Vet Rec"},{"key":"pone.0268247.ref008","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1051\/vetres:2003022","article-title":"Coliform mastitis.","volume":"34","author":"J Hogan","year":"2003","journal-title":"BMC Vet Res"},{"key":"pone.0268247.ref009","first-page":"p1462","volume-title":"Veterinary Medicine: Text book of the disease of cattle, sheep, goat, pigs and horses.","author":"OM Radostits","year":"2007","edition":"1"},{"key":"pone.0268247.ref010","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1089\/fpd.2009.0425","article-title":"Distribution and antimicrobial resistance of clinical and subclinical mastitis pathogens in dairy cows in Rhone-Alpes, France.","volume":"7","author":"M-A Botrel","year":"2010","journal-title":"Foodborne Pathog Dis"},{"key":"pone.0268247.ref011","unstructured":"Jones GM, Bailey TL. Understanding the basics of mastitis. Virginia Cooperative Extension. Publication 404\u2013234. 2009. https:\/\/vtechworks.lib.vt.edu\/bitstream\/handle\/10919\/48392\/404-233_pdf.pdf?sequence=1"},{"issue":"2","key":"pone.0268247.ref012","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.cvfa.2012.03.001","article-title":"New perspectives in udder health management.","volume":"28","author":"PL Ruegg","year":"2012","journal-title":"Vet Clin North Am Food Anim Pract"},{"key":"pone.0268247.ref013","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1128\/JCM.39.5.1845-1849.2001","article-title":"Adaptation of Escherichia coli to the bovine mammary gland.","volume":"39","author":"AJ Bradley","year":"2001","journal-title":"J Clinical Microbiol"},{"key":"pone.0268247.ref014","doi-asserted-by":"crossref","first-page":"980","DOI":"10.2460\/javma.1991.198.06.980","article-title":"Theory, use and realities of efficacy and food safety of antimicrobial treatment of acute coliform mastitis","volume":"6","author":"RJ Erskine","year":"1991","journal-title":"J Am Vet Med Assoc"},{"key":"pone.0268247.ref015","first-page":"511","volume-title":"Antimicrobial Therapy in Veterinary Medicine","author":"S Wagner","year":"2006","edition":"4"},{"key":"pone.0268247.ref016","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.vetmic.2007.04.040","article-title":"Phenotypic and genotypic antimicrobial resistance patterns of Escherichia coli isolated from dairy cows with mastitis","volume":"124","author":"V Srinivasan","year":"2007","journal-title":"Vet Microbiol"},{"key":"pone.0268247.ref017","doi-asserted-by":"crossref","first-page":"4319","DOI":"10.3168\/jds.2012-5373","article-title":"Antimicrobial resistance profiles of common mastitis pathogens on Canadian dairy farms","volume":"95","author":"V Saini","year":"2012","journal-title":"J Dairy Sci"},{"issue":"5","key":"pone.0268247.ref018","doi-asserted-by":"crossref","first-page":"e63413","DOI":"10.1371\/journal.pone.0063413","article-title":"Prevalence and antimicrobial susceptibility patterns of bacteria from milkmen and cows with clinical mastitis in and around Kampala, Uganda.","volume":"8","author":"DP Kateete","year":"2013","journal-title":"PLoSONE"},{"key":"pone.0268247.ref019","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/jac\/38.1.1","article-title":"Does the use of antimicrobial agents in veterinary medicine and animal husbandry select antibiotic-resistant bacteria that infect man and compromise antimicrobial chemotherapy?","volume":"38","author":"LJ Piddock","year":"1996","journal-title":"J Antimicrob Chemother"},{"key":"pone.0268247.ref020","unstructured":"International Development Bank. Project appraisal document on a proposed credit to the republic of Cameroon for a livestock development project document of World Bank, Report No: PAD1664 2016."},{"key":"pone.0268247.ref021","unstructured":"FAO-Food and Agriculture Organization of the United Nations. Impact of mastitis on small scale diary production systems, Animal Production and Health Working Paper. No 13. Rome. 2014"},{"issue":"1","key":"pone.0268247.ref022","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.prevetmed.2006.01.009","article-title":"Clinical and subclinical mastitis in smallholder dairy farms in Tanzania: risk, intervention and knowledge transfer","volume":"74","author":"ED Karimuribo","year":"2005","journal-title":"Prev Vet Med"},{"issue":"4","key":"pone.0268247.ref023","first-page":"332","article-title":"Dairy cattle mastitis in and around Sebeta","volume":"3","author":"H Sori","year":"2005","journal-title":"Ethiopia. Intern J Appl Res Vet Med"},{"key":"pone.0268247.ref024","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.vetmic.2008.05.012","article-title":"Identification of a bovine mastitis Escherichia coli subset","volume":"132","author":"S Blum","year":"2008","journal-title":"Vet Microbiol"},{"issue":"1","key":"pone.0268247.ref025","first-page":"51","article-title":"Towards a sustainable land-use option in the Bamenda highlands, Cameroon: Implication for climate change mitigation, income generation and sustainable food supply.","volume":"3","author":"MI Ndoh","year":"2008","journal-title":"Res J Appl Sci"},{"key":"pone.0268247.ref026","article-title":"Milk production in Cameroon: A review.","volume":"17","author":"60","year":"2005","journal-title":"Livestock Research for Rural Development"},{"key":"pone.0268247.ref027","first-page":"182","author":"M. Thrusfield","year":"2005","edition":"2"},{"key":"pone.0268247.ref028","author":"R. Mellenberger","year":"2001","journal-title":"California mastitis Test: an invaluable tool for managing mastitis"},{"key":"pone.0268247.ref029","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1186\/s12917-016-0905-3","article-title":"Bovine mastitis: prevalence, risk factors and isolation of Staphylococcus aureus in dairy herds at Hawassa milk shed","volume":"12","author":"R Abebe","year":"2016","journal-title":"South Ethiopia. BMC Vet Res"},{"key":"pone.0268247.ref030","unstructured":"NMC-National Mastitis Council. Laboratory Handbook on Bovine Mastitis, 3rd Edition. 2017. http:\/\/www.nmconline.org\/sampling.htm."},{"key":"pone.0268247.ref031","unstructured":"Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing, 30th Edition. 2020. Em100.edaptivedocs.net."},{"key":"pone.0268247.ref032","doi-asserted-by":"crossref","first-page":"1460","DOI":"10.1093\/jac\/dky043","article-title":"Applying definition for multidrug resistance, extensive drug resistance and pandrug resistance to clinically significant livestock and companion animal bacterial pathogens","volume":"73","author":"MT Sweeney","year":"2018","journal-title":"J Antimicrob Chemother"},{"key":"pone.0268247.ref033","doi-asserted-by":"crossref","unstructured":"Peek FP, Divers TJ. Rebhun\u2019s Diseases of Dairy Cattle, 3rd Edition. 2017. www.sciencedirect.com.","DOI":"10.1016\/B978-0-323-39055-2.03001-1"},{"issue":"6","key":"pone.0268247.ref034","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1007\/s11250-018-1629-0","article-title":"Diagnosis of bovine mastitis: from laboratory to farm.","volume":"50","author":"A Ashraf","year":"2018","journal-title":"Trop Anim Health Prod"},{"key":"pone.0268247.ref035","article-title":"Bacterial pathogens involved in bovine mastitis and their antibiotic resistance patterns in the Adamawa region of Cameroon","author":"VN Ngwa","year":"2020","journal-title":"Journal of Dairy Research and Technology"},{"issue":"1","key":"pone.0268247.ref036","doi-asserted-by":"crossref","first-page":"13","DOI":"10.5897\/JVMAH2013.0259","article-title":"Prevalence of clinical and subclinical mastitis on cross bred dairy cows at Holeta Agricultural Research Center, Central Ethiopia.","volume":"6","author":"A Duguma","year":"2014","journal-title":"J Vet Med Anim Health"},{"key":"pone.0268247.ref037","doi-asserted-by":"crossref","first-page":"1507","DOI":"10.1007\/s11250-019-01838-w","article-title":"Risk factors for bovine mastitis with the isolation and identification of Streptococcus agalactiae from farms in and around Haramaya District, Eastern Ethiopia.","volume":"51","author":"BT Lakew","year":"2019","journal-title":"Trop Anim Health Prod"},{"issue":"1","key":"pone.0268247.ref038","doi-asserted-by":"crossref","first-page":"523","DOI":"10.4102\/ojvr.v80i1.523","article-title":"Prevalence of mastitis in dairy cows from smallholder farms in Zimbabwe","volume":"80","author":"S Katsande","year":"2013","journal-title":"Onderstepoort J Vet Res"},{"key":"pone.0268247.ref039","doi-asserted-by":"crossref","first-page":"05","DOI":"10.5455\/wvj.20130223","article-title":"Risk factors associated with mastitis occurrence in dairy herds in Beni-Suef Governorate.","volume":"3","author":"MA Elbably","year":"2013","journal-title":"World\u2019s Vet J"},{"issue":"11","key":"pone.0268247.ref040","first-page":"10","article-title":"Prevalence of mastitis and identification of its bacterial causative agents in small holder dairy farms in and around Wukro of Tigray region, Ethiopia.","volume":"5","author":"TF Demissie","year":"2018","journal-title":"Int J Adv Res Biol Sci"},{"issue":"3","key":"pone.0268247.ref041","first-page":"36","article-title":"The prevalence of clinical and subclinical mastitis in dairy cows in the central region of Fars province, South of Iran.","volume":"12","author":"M Hashemi","year":"2011","journal-title":"Iranian Journal of Veterinary Research, Shiraz University."},{"issue":"1","key":"pone.0268247.ref042","first-page":"50","article-title":"Study on prevalence, bacterial pathogens and associated risk factors of bovine mastitis in small holder dairy farms in and around Addis Ababa","volume":"23","author":"AT Zeryehun","year":"2013","journal-title":"Ethiopia. J Anim Plant Sci"},{"issue":"2","key":"pone.0268247.ref043","doi-asserted-by":"crossref","first-page":"1","DOI":"10.9734\/ajarr\/2019\/v3i230084","article-title":"Antimicrobial susceptibility profile of coliforms from bovine mastitis cases among pastoral herds in parts of Kaduna State, Nigeria: Curbing the environmental health risk.","volume":"3","author":"D Makolo","year":"2019","journal-title":"Asian J Adv Res Reports"},{"issue":"1","key":"pone.0268247.ref044","doi-asserted-by":"crossref","DOI":"10.4102\/jsava.v86i1.1228","article-title":"Bovine mastitis prevalence and associated risk factors in dairy cows in Nyagatare District, Rwanda.","volume":"86","author":"B Iraguha","year":"2015","journal-title":"J S Afr Vet Assoc"},{"issue":"4","key":"pone.0268247.ref045","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1007\/s10911-011-9234-0","article-title":"Mastitis: Comparative etiology and epidemiology","volume":"16","author":"GA Contreras","year":"2011","journal-title":"J Mammary Gland Biol Neoplasia"},{"issue":"5","key":"pone.0268247.ref046","article-title":"Mastitis occurrence and constraints to mastitis controlling smallholder dairy farming systems in Uganda.","volume":"20","author":"D. K. Byarugaba","year":"2008","journal-title":"Livestock Research for Rural Development"},{"issue":"1","key":"pone.0268247.ref047","doi-asserted-by":"crossref","first-page":"36","DOI":"10.3844\/ajavsp.2008.36.39","article-title":"Prevalence and distribution of mastitis pathogens and their resistance against antimicrobial agents in dairy cows in Jordan.","volume":"3","author":"AD Hawari","year":"2008","journal-title":"Am J Anim Vet Sci"},{"key":"pone.0268247.ref048","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1111\/j.1348-0421.2011.00323.x","article-title":"Molecular characterization of antimicrobial resistance in Gram-negative bacteria isolated from bovine mastitis in Egypt","volume":"55","author":"AM Ahmed","year":"2011","journal-title":"Microbiol Immunol"},{"issue":"12","key":"pone.0268247.ref049","doi-asserted-by":"crossref","first-page":"228","DOI":"10.5897\/JVMAH2016.0522","article-title":"Coliform organisms associated with milk of cows with mastitis and their sensitivity to commonly available antibiotics in Kaduna State","volume":"8","author":"EU Mbuk","year":"2016","journal-title":"Nigeria. 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Taponen","year":"2016","journal-title":"J Dairy Sci"},{"key":"pone.0268247.ref059","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1023\/A:1023352811751","article-title":"Bovine mastitis in selected areas of Southern Ethiopia.","volume":"35","author":"OK Dego","year":"2003","journal-title":"Trop Anim Health Prod"},{"key":"pone.0268247.ref060","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1093\/clind\/15.5.824","article-title":"Beta-lactam resistance in Gram-negative bacteria: Global Trends and Clinical Impact","volume":"15","author":"CC Sanders","year":"1992","journal-title":"Clin Infect Dis"},{"key":"pone.0268247.ref061","first-page":"33","article-title":"Are all beta-lactams created equal?","volume":"101","author":"DM Livermore","year":"1996","journal-title":"Scand J Infect Dis Suppl"},{"issue":"6","key":"pone.0268247.ref062","first-page":"530","volume":"21","author":"FDA-Food and Drug Administration","year":"2012","journal-title":"Extra-label Drug use in 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