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Laundry detergent (LD) that contains cytotoxic ingredients including microbial enzymes continuously comes into contact with the skin starting in infancy. An impaired skin barrier has been suggested as a route of allergic sensitization. We hypothesized that exposure of skin to LD damages the skin barrier resulting in systemic sensitization to allergens that enter through the impaired skin barrier. Mouse skin samples exposed<jats:italic>in vitro<\/jats:italic>to microbial proteases or LD exhibited physical damage, which was more pronounced in neonatal skin as compared to adult skin. Exposure of the skin to microbial proteases<jats:italic>in vitro<\/jats:italic>resulted in an increase in the levels of interleukin (IL)-33 and thymic stromal lymphopoietin (TSLP). BALB\/c wild type mice epicutaneously exposed to LD and ovalbumin (OVA) showed an increase in levels of transepidermal water loss, serum OVA-specific immunoglobulin (Ig) G1 and IgE antibodies, and a local increase of<jats:italic>Il33<\/jats:italic>,<jats:italic>Tslp<\/jats:italic>,<jats:italic>Il4<\/jats:italic>and<jats:italic>Il13<\/jats:italic>compared with LD or OVA alone. Following intranasal challenge with OVA, mice epicutaneously exposed to LD showed an increase in allergen-induced esophageal eosinophilia compared with LD or OVA alone. Collectively, these results suggest that LD may be an important factor that impairs the skin barrier and leads to allergen sensitization in early life, and therefore may have a role in the increase in allergic disease.<\/jats:p>","DOI":"10.1371\/journal.pone.0268651","type":"journal-article","created":{"date-parts":[[2022,6,27]],"date-time":"2022-06-27T17:25:07Z","timestamp":1656350707000},"page":"e0268651","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":19,"title":["Laundry detergent promotes allergic skin inflammation and esophageal eosinophilia in mice"],"prefix":"10.1371","volume":"17","author":[{"given":"Jamie","family":"Tanzer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Di","family":"Meng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asa","family":"Ohsaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3855-299X","authenticated-orcid":true,"given":"Julie M.","family":"Caldwell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2664-3050","authenticated-orcid":true,"given":"Melissa K.","family":"Mingler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc E.","family":"Rothenberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michiko K.","family":"Oyoshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2022,6,27]]},"reference":[{"key":"pone.0268651.ref001","unstructured":"Pawankar R, Holgate S, Canonica G, Lockey R, Blaiss M. WAO White Book on Allergy 2013 Update 2013. Available from: https:\/\/www.worldallergy.org\/wao-white-book-on-allergy."},{"issue":"10","key":"pone.0268651.ref002","first-page":"1","article-title":"Food allergy among U.S. children: trends in prevalence and hospitalizations","author":"AM Branum","year":"2008","journal-title":"NCHS Data Brief"},{"key":"pone.0268651.ref003","unstructured":"Food Allergy Research & Education. Facts and Statistics 2020. Available from: https:\/\/www.foodallergy.org\/resources\/facts-and-statistics."},{"issue":"1","key":"pone.0268651.ref004","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-2249.2010.04139.x","article-title":"The \u2019hygiene hypothesis\u2019 for autoimmune and allergic diseases: an update","volume":"160","author":"H Okada","year":"2010","journal-title":"Clin Exp Immunol"},{"issue":"2","key":"pone.0268651.ref005","doi-asserted-by":"crossref","first-page":"82","DOI":"10.2310\/6620.2007.06034","article-title":"A population-based survey of eczema prevalence in the United States","volume":"18","author":"Impact Working G","year":"2007","journal-title":"Dermatitis"},{"issue":"1","key":"pone.0268651.ref006","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1136\/thx.2004.038844","article-title":"Time trends in allergic disorders in the UK","volume":"62","author":"R Gupta","year":"2007","journal-title":"Thorax"},{"issue":"4","key":"pone.0268651.ref007","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1002\/ajhb.21043","article-title":"Barrier requirements as the evolutionary \"driver\" of epidermal pigmentation in humans","volume":"22","author":"PM Elias","year":"2010","journal-title":"Am J Hum Biol"},{"issue":"5","key":"pone.0268651.ref008","doi-asserted-by":"crossref","first-page":"S258","DOI":"10.1067\/mai.2000.110159","article-title":"Allergen sensitization through the skin induces systemic allergic responses","volume":"106","author":"LA Beck","year":"2000","journal-title":"J Allergy Clin Immunol"},{"issue":"3","key":"pone.0268651.ref009","doi-asserted-by":"crossref","DOI":"10.1016\/j.jaci.2009.05.042","article-title":"Filaggrin-deficient mice exhibit TH17-dominated skin inflammation and permissiveness to epicutaneous sensitization with protein antigen","volume":"124","author":"MK Oyoshi","year":"2009","journal-title":"J Allergy Clin 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Engl J Med"},{"issue":"17","key":"pone.0268651.ref016","doi-asserted-by":"crossref","first-page":"1640","DOI":"10.1056\/NEJMra1502863","article-title":"Eosinophilic Esophagitis","volume":"373","author":"GT Furuta","year":"2015","journal-title":"N Engl J Med"},{"issue":"3","key":"pone.0268651.ref017","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.jaad.2016.08.068","article-title":"Prevalence of atopic comorbidities in eosinophilic esophagitis: A case-control study of 449 patients","volume":"76","author":"AA Mohammad","year":"2017","journal-title":"J Am Acad Dermatol"},{"issue":"545","key":"pone.0268651.ref018","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.aaz7773","article-title":"Functional role of kallikrein 5 and proteinase-activated receptor 2 in eosinophilic esophagitis","volume":"12","author":"NP Azouz","year":"2020","journal-title":"Sci Transl 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Dellon","year":"2018","journal-title":"Gastroenterology"},{"issue":"2","key":"pone.0268651.ref022","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.jaip.2016.11.020","article-title":"The Prevalence of Eosinophilic Esophagitis in Pediatric Patients with IgE-Mediated Food Allergy","volume":"5","author":"DA Hill","year":"2017","journal-title":"J Allergy Clin Immunol Pract"},{"issue":"6","key":"pone.0268651.ref023","doi-asserted-by":"crossref","first-page":"1660","DOI":"10.1053\/j.gastro.2003.09.024","article-title":"Natural history of primary eosinophilic esophagitis: a follow-up of 30 adult patients for up to 11.5 years","volume":"125","author":"A Straumann","year":"2003","journal-title":"Gastroenterology"},{"issue":"1","key":"pone.0268651.ref024","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/1939-4551-6-21","article-title":"A global survey of changing patterns of food allergy burden in children","volume":"6","author":"SL Prescott","year":"2013","journal-title":"World Allergy Organ J"},{"issue":"2","key":"pone.0268651.ref025","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1111\/j.1525-1470.2009.00973.x","article-title":"Infant skin microstructure assessed in vivo differs from adult skin in organization and at the cellular level","volume":"27","author":"GN Stamatas","year":"2010","journal-title":"Pediatr Dermatol"},{"issue":"8","key":"pone.0268651.ref026","doi-asserted-by":"crossref","first-page":"1892","DOI":"10.1038\/jid.2009.133","article-title":"Epidermal barrier dysfunction in atopic dermatitis","volume":"129","author":"MJ Cork","year":"2009","journal-title":"J Invest Dermatol"},{"issue":"6","key":"pone.0268651.ref027","doi-asserted-by":"crossref","first-page":"432","DOI":"10.2340\/0001555572432435","article-title":"A dose-response study of irritant reactions to sodium lauryl sulphate in patients with seborrhoeic dermatitis and atopic eczema","volume":"72","author":"NC Cowley","year":"1992","journal-title":"Acta Derm Venereol"},{"issue":"9","key":"pone.0268651.ref028","first-page":"1120","article-title":"Respiratory disease in industry due to B. subtilis enzyme preparations","volume":"108","author":"DC Little","year":"1973","journal-title":"Can Med Assoc J"},{"issue":"3","key":"pone.0268651.ref029","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/S0958-1669(02)00317-8","article-title":"Low-temperature extremophiles and their applications","volume":"13","author":"R Cavicchioli","year":"2002","journal-title":"Curr Opin Biotechnol"},{"issue":"4","key":"pone.0268651.ref030","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.ijbiomac.2008.09.018","article-title":"Co-solvent effects on structure and function properties of savinase: solvent-induced thermal stabilization","volume":"44","author":"A Nasiripourdori","year":"2009","journal-title":"Int J Biol Macromol"},{"key":"pone.0268651.ref031","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1002\/3527602135.ch5b","volume-title":"Industrial Enzymes: Enzymes in Nonfood Applications","author":"R Antrim","year":"2003"},{"issue":"7607","key":"pone.0268651.ref032","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1016\/S0140-6736(69)92165-5","article-title":"Pulmonary disease due to inhalation of derivatives of Bacillus subtilis containing proteolytic enzyme","volume":"1","author":"ML Flindt","year":"1969","journal-title":"Lancet"},{"issue":"7607","key":"pone.0268651.ref033","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1016\/S0140-6736(69)92166-7","article-title":"Allergic reactions of the lungs to enzymes of Bacillus subtilis","volume":"1","author":"J Pepys","year":"1969","journal-title":"Lancet"},{"issue":"7649","key":"pone.0268651.ref034","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1016\/S0140-6736(70)90924-4","article-title":"An epidemiological study of workers producing enzyme washing powders","volume":"1","author":"ML Newhouse","year":"1970","journal-title":"Lancet"},{"issue":"1","key":"pone.0268651.ref035","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/j.1398-9995.1972.tb01644.x","article-title":"Medical problems in the detergent industry caused by proteolytic enzymes from bacillus subtilis","volume":"27","author":"CJ Gothe","year":"1972","journal-title":"Acta Allergol"},{"issue":"5691","key":"pone.0268651.ref036","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1136\/bmj.1.5691.299","article-title":"Severe dermatitis and \"biological\" detergents","volume":"1","author":"NE Jensen","year":"1970","journal-title":"Br Med J"},{"issue":"5695","key":"pone.0268651.ref037","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1136\/bmj.1.5695.537","article-title":"Contact dermatitis in home helps following the use of enzyme detergents","volume":"1","author":"CF Ducksbury","year":"1970","journal-title":"Br Med J"},{"issue":"5696","key":"pone.0268651.ref038","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1136\/bmj.1.5696.629","article-title":"Severe dermatitis and \"biological\" detergents","volume":"1","author":"E Bamji","year":"1970","journal-title":"Br Med J"},{"issue":"6","key":"pone.0268651.ref039","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/S0015-6264(69)80461-X","article-title":"Safety evaluation of enzyme detergents. Oral and cutaneous toxicity, irritancy and skin sensitization studies","volume":"7","author":"JF Griffith","year":"1969","journal-title":"Food Cosmet Toxicol"},{"issue":"2","key":"pone.0268651.ref040","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0091-6749(98)70382-3","article-title":"Safety assessment of enzyme-containing personal cleansing products: exposure characterization and development of IgE antibody to enzymes after a 6-month use test","volume":"101","author":"CK Kelling","year":"1998","journal-title":"J Allergy Clin Immunol"},{"issue":"2","key":"pone.0268651.ref041","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1006\/faat.1996.0027","article-title":"Specific IgE and IgG1 responses to subtilisin Carlsberg (Alcalase) in mice: development of an intratracheal exposure model","volume":"29","author":"TT Kawabata","year":"1996","journal-title":"Fundam Appl Toxicol"},{"issue":"1","key":"pone.0268651.ref042","first-page":"39","article-title":"Use of the mouse intranasal test (MINT) to determine the allergenic potency of detergent enzymes: comparison to the guinea pig intratracheal (GPIT) test","volume":"43","author":"MK Robinson","year":"1998","journal-title":"Toxicol Sci"},{"issue":"4","key":"pone.0268651.ref043","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/0091-6749(72)90085-1","article-title":"Enzyme allergy in populations exposed to long-term, low-level concentrations of household laundry products","volume":"49","author":"IL Bernstein","year":"1972","journal-title":"J Allergy Clin Immunol"},{"key":"pone.0268651.ref044","unstructured":"Star E. About Energy Star 1992. Available from: https:\/\/www.energystar.gov\/index.cfm?c=about.ab_index."},{"key":"pone.0268651.ref045","unstructured":"Energy. USEPADo. Market Impact Analysis of Potential Changes to the ENERGY STAR\u00ae Criteria for Clothes Washers 2004. Available from: https:\/\/www.energystar.gov\/ia\/partners\/prod_development\/revisions\/downloads\/clotheswash\/2ESCWCriteriaAnalysisFinal.pdf."},{"key":"pone.0268651.ref046","unstructured":"Star E. ENERGY STAR History and Major Milestones 2019. Available from: https:\/\/www.energystar.gov\/about\/history\/major-milestones."},{"key":"pone.0268651.ref047","unstructured":"Which? How we test washing machines 2019. Available from: https:\/\/www.which.co.uk\/reviews\/washing-machines\/article\/how-we-test-washing-machines-aQOn95P2K1rC."},{"issue":"7","key":"pone.0268651.ref048","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1038\/ni805","article-title":"Human epithelial cells trigger dendritic cell mediated allergic inflammation by producing TSLP","volume":"3","author":"V Soumelis","year":"2002","journal-title":"Nat Immunol"},{"issue":"5","key":"pone.0268651.ref049","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.immuni.2005.09.015","article-title":"IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines","volume":"23","author":"J Schmitz","year":"2005","journal-title":"Immunity"},{"issue":"2","key":"pone.0268651.ref050","doi-asserted-by":"crossref","first-page":"e31967","DOI":"10.1371\/journal.pone.0031967","article-title":"Epicutaneous immunotherapy (EPIT) blocks the allergic esophago-gastro-enteropathy induced by sustained oral exposure to peanuts in sensitized mice","volume":"7","author":"L Mondoulet","year":"2012","journal-title":"PLoS ONE"},{"issue":"6","key":"pone.0268651.ref051","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1016\/j.jaci.2014.12.1923","article-title":"Filaggrin deficiency promotes the dissemination of cutaneously inoculated vaccinia virus","volume":"135","author":"MK Oyoshi","year":"2015","journal-title":"J Allergy Clin Immunol"},{"key":"pone.0268651.ref052","unstructured":"Conway J. Sales share of the leading 10 liquid laundry detergent brands of the United States in 2018 2018. Available from: https:\/\/www.statista.com\/statistics\/251472\/sales-share-of-the-leading-10-liquid-laundry-detergent-brands-of-the-us\/."},{"key":"pone.0268651.ref053","unstructured":"Panandiker RK, Vetter KA, Combs MJ, Gladney D, Sheets CL, Frankenbach GM, et al., inventors; Procter and Gamble Co., assignee. Liquid laundry detergent compositions comprising performance boosters European Patent Office 2008."},{"key":"pone.0268651.ref054","unstructured":"HERA. Human & Environmental Risk Assessment on ingredients of household cleaning products\u2014Subtilisins (Proteases) 2007. 2.0:[Available from: www.heraproject.com."},{"issue":"11","key":"pone.0268651.ref055","doi-asserted-by":"crossref","first-page":"6273","DOI":"10.1073\/pnas.95.11.6273","article-title":"Eotaxin is required for the baseline level of tissue eosinophils","volume":"95","author":"AN Matthews","year":"1998","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5","key":"pone.0268651.ref056","doi-asserted-by":"crossref","DOI":"10.1016\/j.jaci.2016.02.034","article-title":"Allergic skin sensitization promotes eosinophilic esophagitis through the IL-33-basophil axis in mice","volume":"138","author":"N Venturelli","year":"2016","journal-title":"J Allergy Clin Immunol"},{"issue":"1","key":"pone.0268651.ref057","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1084\/jem.20171163","article-title":"Maternal IgG immune complexes induce food allergen-specific tolerance in offspring","volume":"215","author":"A Ohsaki","year":"2018","journal-title":"J Exp Med"},{"issue":"2","key":"pone.0268651.ref058","doi-asserted-by":"crossref","DOI":"10.1016\/j.jaci.2012.11.032","article-title":"Epicutaneous sensitization results in IgE-dependent intestinal mast cell expansion and food-induced anaphylaxis","volume":"131","author":"LM Bartnikas","year":"2013","journal-title":"J Allergy Clin Immunol"},{"issue":"12","key":"pone.0268651.ref059","doi-asserted-by":"crossref","first-page":"2719","DOI":"10.1038\/sj.jid.5700584","article-title":"Reduction of skin barrier function by proteolytic activity of a recombinant house dust mite major allergen Der f 1","volume":"126","author":"T Nakamura","year":"2006","journal-title":"J Invest Dermatol"},{"issue":"5","key":"pone.0268651.ref060","doi-asserted-by":"crossref","DOI":"10.1016\/j.jaci.2010.08.041","article-title":"Mechanical injury polarizes skin dendritic cells to elicit a T(H)2 response by inducing cutaneous thymic stromal 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Fuchs","year":"2007","journal-title":"Nature"},{"issue":"1","key":"pone.0268651.ref074","doi-asserted-by":"crossref","first-page":"2","DOI":"10.4103\/0975-7406.92724","article-title":"Status of surfactants as penetration enhancers in transdermal drug delivery","volume":"4","author":"I Som","year":"2012","journal-title":"J Pharm Bioallied Sci"},{"issue":"5","key":"pone.0268651.ref075","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1111\/j.0105-1873.2005.00606.x","article-title":"Allergic contact dermatitis from ophthalmic products: can pre-treatment with sodium lauryl sulfate increase patch test sensitivity?","volume":"52","author":"M Corazza","year":"2005","journal-title":"Contact Dermatitis"},{"issue":"5","key":"pone.0268651.ref076","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1111\/j.0105-1873.2005.00703.x","article-title":"Long-term repetitive sodium lauryl sulfate-induced irritation of the skin: an in vivo study","volume":"53","author":"N Branco","year":"2005","journal-title":"Contact Dermatitis"},{"issue":"12","key":"pone.0268651.ref077","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1111\/j.1600-0625.2009.00877.x","article-title":"Sodium lauryl sulphate alters the mRNA expression of lipid-metabolizing enzymes and PPAR signalling in normal human skin in vivo","volume":"18","author":"H Torma","year":"2009","journal-title":"Exp Dermatol"},{"issue":"8","key":"pone.0268651.ref078","doi-asserted-by":"crossref","first-page":"1387","DOI":"10.1016\/j.tiv.2006.06.002","article-title":"Detergents with different chemical properties induce variable degree of cytotoxicity and mRNA expression of lipid-metabolizing enzymes and differentiation markers in cultured keratinocytes","volume":"20","author":"T Wei","year":"2006","journal-title":"Toxicol In Vitro"},{"issue":"3","key":"pone.0268651.ref079","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.clindermatol.2011.08.021","article-title":"Effect of soaps and detergents on epidermal barrier function","volume":"30","author":"R Wolf","year":"2012","journal-title":"Clin Dermatol"},{"issue":"9","key":"pone.0268651.ref080","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.tibs.2009.08.001","article-title":"The emerging roles of serine protease cascades in the epidermis","volume":"34","author":"P Ovaere","year":"2009","journal-title":"Trends Biochem Sci"},{"issue":"1","key":"pone.0268651.ref081","doi-asserted-by":"crossref","DOI":"10.1016\/j.jaci.2010.10.039","article-title":"A striking local esophageal cytokine expression profile in eosinophilic esophagitis","volume":"127","author":"C Blanchard","year":"2011","journal-title":"J Allergy Clin Immunol"},{"issue":"4","key":"pone.0268651.ref082","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1038\/ng.547","article-title":"Common variants at 5q22 associate with pediatric eosinophilic esophagitis","volume":"42","author":"ME Rothenberg","year":"2010","journal-title":"Nat 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Commun"},{"issue":"9","key":"pone.0268651.ref086","doi-asserted-by":"crossref","first-page":"2185","DOI":"10.1111\/all.14304","article-title":"Epicutaneous sensitization in the development of food allergy: What is the evidence and how can this be prevented?","volume":"75","author":"HA Brough","year":"2020","journal-title":"Allergy"},{"key":"pone.0268651.ref087","article-title":"Advances and novel developments in mechanisms of allergic inflammation","author":"X Han","year":"2020","journal-title":"Allergy"},{"issue":"4","key":"pone.0268651.ref088","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1016\/S0091-6749(97)70139-8","article-title":"Proteolytic detergent enzymes enhance the allergic antibody responses of guinea pigs to nonproteolytic detergent enzymes in a mixture: implications for occupational exposure","volume":"100","author":"K Sarlo","year":"1997","journal-title":"J Allergy Clin Immunol"},{"issue":"10","key":"pone.0268651.ref089","doi-asserted-by":"crossref","first-page":"5904","DOI":"10.4049\/jimmunol.169.10.5904","article-title":"A protease-activated pathway underlying Th cell type 2 activation and allergic lung disease","volume":"169","author":"F Kheradmand","year":"2002","journal-title":"J Immunol"},{"issue":"5","key":"pone.0268651.ref090","doi-asserted-by":"crossref","first-page":"1892","DOI":"10.1016\/j.jaci.2018.11.016","article-title":"Laundry detergents and detergent residue after rinsing directly disrupt tight junction barrier integrity in human bronchial epithelial cells","volume":"143","author":"M Wang","year":"2019","journal-title":"J Allergy Clin Immunol"},{"issue":"10","key":"pone.0268651.ref091","doi-asserted-by":"crossref","first-page":"1316","DOI":"10.1038\/s41590-021-01011-2","article-title":"Environmental allergens trigger type 2 inflammation through ripoptosome activation","volume":"22","author":"M Brusilovsky","year":"2021","journal-title":"Nat Immunol"},{"issue":"1","key":"pone.0268651.ref092","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/S0190-9622(96)90495-8","article-title":"Skin reactivity to sodium lauryl sulfate in patients with respiratory atopy","volume":"35","author":"S Seidenari","year":"1996","journal-title":"J Am Acad Dermatol"},{"issue":"1","key":"pone.0268651.ref093","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1111\/j.1365-2133.2006.07319.x","article-title":"Percutaneous penetration of sodium lauryl sulphate is increased in uninvolved skin of patients with atopic dermatitis compared with control subjects","volume":"155","author":"I Jakasa","year":"2006","journal-title":"Br J Dermatol"},{"issue":"8","key":"pone.0268651.ref094","article-title":"ZAG Regulates the Skin Barrier and Immunity in Atopic Dermatitis","volume":"139","author":"JY Noh","year":"2019","journal-title":"J Invest Dermatol"},{"issue":"23","key":"pone.0268651.ref095","doi-asserted-by":"crossref","first-page":"8816","DOI":"10.1073\/pnas.0602900103","article-title":"Contribution of IL-18 to atopic-dermatitis-like skin inflammation induced by Staphylococcus aureus product in mice","volume":"103","author":"M Terada","year":"2006","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5","key":"pone.0268651.ref096","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1159\/000231627","article-title":"Influence of detergent on aerosol allergic sensitization with enzymes of Bacillus subtilis","volume":"51","author":"RJ Markham","year":"1976","journal-title":"Int Arch Allergy Appl Immunol"},{"issue":"1","key":"pone.0268651.ref097","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/0041-008X(80)90248-3","article-title":"Immunologic responses of cynomolgus monkeys after repeated inhalation exposures to enzymes and enzyme\u2014detergent mixtures","volume":"52","author":"F Cashner","year":"1980","journal-title":"Toxicol Appl Pharmacol"},{"key":"pone.0268651.ref098","article-title":"Does the epithelial barrier hypothesis explain the increase in allergy, autoimmunity and other chronic conditions?","author":"CA Akdis","year":"2021","journal-title":"Nature Reviews Immunology"},{"key":"pone.0268651.ref099","first-page":"2296","author":"R Doucet-Ladev\u00e8ze","journal-title":"Transcriptomic Analysis Links Eosinophilic Esophagitis and Atopic Dermatitis"},{"key":"pone.0268651.ref100","first-page":"2213","author":"DA Hill","journal-title":"Eosinophilic Esophagitis Is a Late Manifestation of the Allergic March"},{"issue":"10","key":"pone.0268651.ref101","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1089\/wound.2015.0642","article-title":"Skin Physiology of the Neonate and Infant: Clinical Implications","volume":"4","author":"T Oranges","year":"2015","journal-title":"Adv Wound Care (New Rochelle)"},{"key":"pone.0268651.ref102","article-title":"Very Early Onset Eosinophilic Esophagitis is Common, Responds to Standard Therapy, and Demonstrates Enrichment for CAPN14 Genetic Variants","author":"J Lyles","year":"2020","journal-title":"J Allergy Clin Immunol"},{"issue":"2","key":"pone.0268651.ref103","doi-asserted-by":"crossref","first-page":"e163909","DOI":"10.1001\/jamapediatrics.2016.3909","article-title":"Cost-effectiveness of Prophylactic Moisturization for Atopic Dermatitis","volume":"171","author":"S Xu","year":"2017","journal-title":"JAMA Pediatr"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0268651","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T20:22:16Z","timestamp":1700770936000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0268651"}},"subtitle":[],"editor":[{"given":"Svetlana 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