{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T09:41:07Z","timestamp":1774690867912,"version":"3.50.1"},"reference-count":48,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T00:00:00Z","timestamp":1654560000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["FWF P 30061"],"award-info":[{"award-number":["FWF P 30061"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Temperate zone ungulates like red deer (<jats:italic>Cervus elaphus<\/jats:italic>) show pronounced seasonal acclimatisation. Hypometabolism during winter is associated with cardiovascular changes, including a reduction in heart rate (<jats:italic>f<\/jats:italic><jats:sub>H<\/jats:sub>) and temporal peripheral vasoconstriction. How anaesthesia with vasoactive substances such as medetomidine affect the seasonally acclimatised cardiovascular system is not yet known. We anaesthetised eleven healthy female red deer with medetomidine (0.1 mg\/kg) and tiletamine\/zolazepam (3 mg\/kg) twice in winter (<jats:italic>ad libitum<\/jats:italic>and restricted feed) and in summer (<jats:italic>ad libitum<\/jats:italic>and restricted feed), with a two-week washout-period in-between, to test for the effect of season, food availability and supplementation with omega-3 or omega-6 polyunsaturated fatty acid (PUFA) on<jats:italic>f<\/jats:italic><jats:sub>H<\/jats:sub>and arterial blood pressure (ABP) during anaesthesia. Six animals received pellets enriched with omega-6 fatty acids (FA), and five animals with omega-3 FA. Anaesthesia significantly decreased<jats:italic>f<\/jats:italic><jats:sub>H<\/jats:sub>in summer but not in winter and ABP was lower in winter (<jats:italic>p<\/jats:italic>&lt; 0.05). The combination of omega-6 FA enriched pellets and food restriction resulted in a lower<jats:italic>f<\/jats:italic><jats:sub>H<\/jats:sub>and higher ABP during anaesthesia with more pronounced changes in winter (<jats:italic>p<\/jats:italic>&lt; 0.001). Our results demonstrate that season, food availability and type of PUFA supplementation in red deer affect the cardiovascular system during anaesthesia.<\/jats:p>","DOI":"10.1371\/journal.pone.0268811","type":"journal-article","created":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T17:30:54Z","timestamp":1654623054000},"page":"e0268811","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Effect of season and diet on heart rate and blood pressure in female red deer (Cervus elaphus) anaesthetised with medetomidine-tiletamine-zolazepam"],"prefix":"10.1371","volume":"17","author":[{"given":"Hanna","family":"Rauch","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Friederike","family":"Pohlin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joy","family":"Einwaller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuela","family":"Habe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristina","family":"Gasch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Haw","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walter","family":"Arnold","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8901-1181","authenticated-orcid":true,"given":"Gabrielle","family":"Stalder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3929-511X","authenticated-orcid":true,"given":"Johanna","family":"Painer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2022,6,7]]},"reference":[{"issue":"4","key":"pone.0268811.ref001","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1086\/504614","article-title":"Physiological adjustments of Sand Gazelles (Gazella subgutturosa) to a boom-or-bust economy: Standard fasting metabolic rate, total evaporative water loss, and changes in the sizes of organs during food and water restriction","volume":"79","author":"S Ostrowski","year":"2006","journal-title":"Physiol Biochem Zool"},{"issue":"1","key":"pone.0268811.ref002","doi-asserted-by":"crossref","first-page":"2142","DOI":"10.1038\/s41598-018-20655-z","article-title":"Free-living greylag geese adjust their heart rates and body core temperatures to season and reproductive context","volume":"8","author":"CAF Wascher","year":"2018","journal-title":"Sci Rep"},{"issue":"S1","key":"pone.0268811.ref003","doi-asserted-by":"crossref","first-page":"s124","DOI":"10.1017\/S1751731119003240","article-title":"Review: Seasonal differences in the physiology of wild northern ruminants","volume":"14","author":"W. 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