{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T18:32:42Z","timestamp":1788719562717,"version":"build-2803163510"},"reference-count":44,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2022,5,26]],"date-time":"2022-05-26T00:00:00Z","timestamp":1653523200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["Afrique One-ASPIRE\/ DEL-15-008"],"award-info":[{"award-number":["Afrique One-ASPIRE\/ DEL-15-008"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["Afrique One-ASPIRE\/ DEL-15-008"],"award-info":[{"award-number":["Afrique One-ASPIRE\/ DEL-15-008"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["Afrique One-ASPIRE\/ DEL-15-008"],"award-info":[{"award-number":["Afrique One-ASPIRE\/ DEL-15-008"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["Afrique One-ASPIRE\/ DEL-15-008"],"award-info":[{"award-number":["Afrique One-ASPIRE\/ DEL-15-008"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Introduction<\/jats:title>\n<jats:p>Diarrhoea accounts for high morbidity and mortality in children and adults worldwide. Extended Spectrum Beta-Lactamase-Producing Enterobacteriaceae (ESBL-PE) and Diarrhoeagenic <jats:italic>Escherichia coli<\/jats:italic> (DEC) contribute to prolonged hospitalization because of their resistance and virulence properties aiding in the spread of diarrhoeal disease and delayed treatment.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Aim<\/jats:title>\n<jats:p>To determine DEC and the antimicrobial resistance of ESBL-PE isolated among diarrhoea patients attending two health facilities in Ghana.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Methods<\/jats:title>\n<jats:p>Stool samples were collected from 122 diarrhoeal patients who attended Maamobi General Hospital and Kaneshie Polyclinic between January 2019 and March 2020. Identification of bacteria was performed by using the Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Using disk diffusion, antimicrobial susceptibility testing (AST) was conducted and interpreted according to the 2018 CLSI guidelines. Detection of ESBL and DEC genes was performed using Polymerase chain reaction (PCR).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Results<\/jats:title>\n<jats:p>A total of 80.3% (98\/122) Enterobacteriaceae was recovered from the patients in the study with an overall ESBL occurrence of 20.4% (20\/98), predominantly among <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> showed 13.2% (10\/76), <jats:italic>Klebsiella pneumoniae<\/jats:italic>,35.7%(5\/14) and <jats:italic>Proteus mirabilis<\/jats:italic>, 57.1%(4\/7). Among the ESBL genes detected, <jats:italic>bla<\/jats:italic><jats:sub><jats:italic>TEM<\/jats:italic><\/jats:sub> (n = 14) was common, followed by <jats:italic>bla<\/jats:italic><jats:sub><jats:italic>CTX-M<\/jats:italic><\/jats:sub> (n = 13) and <jats:italic>bla<\/jats:italic><jats:sub><jats:italic>SHV<\/jats:italic><\/jats:sub> (n = 4). Thirty-four <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> isolates possessed the heat labile (Lt) gene of Enterotoxigenic <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> (ETEC).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec005\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>Our findings confirm the existence of DEC and the antimicrobial resistance patterns of ESBL-PE among stool isolates, limiting the options of commonly used drugs for diarrhoeal treatment in Ghana. Routine laboratory testing in health care facilities and strengthened surveillance systems among hospital networks are encouraged for a better understanding of their epidemiology and clinical implications.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0268991","type":"journal-article","created":{"date-parts":[[2022,5,26]],"date-time":"2022-05-26T17:47:36Z","timestamp":1653587256000},"page":"e0268991","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":36,"title":["Diarrhoeagenic E. coli occurrence and antimicrobial resistance of Extended Spectrum Beta-Lactamases isolated from diarrhoea patients attending health facilities in Accra, Ghana"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8118-9965","authenticated-orcid":true,"given":"Helena","family":"Dela","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beverly","family":"Egyir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4986-8284","authenticated-orcid":true,"given":"Ayodele O.","family":"Majekodunmi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Behene","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Yeboah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominic","family":"Ackah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard N. 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