{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:38:03Z","timestamp":1792802283596,"version":"4.5.0"},"reference-count":64,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2022,6,15]],"date-time":"2022-06-15T00:00:00Z","timestamp":1655251200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100017700","name":"Henan Provincial Science and Technology Research Project","doi-asserted-by":"publisher","award":["222102310375"],"award-info":[{"award-number":["222102310375"]}],"id":[{"id":"10.13039\/501100017700","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Henan University of Chinese Medicine In-school Support projects","award":["MP2021-14"],"award-info":[{"award-number":["MP2021-14"]}],"id":[{"id":"https:\/\/ror.org\/01tsmvz08","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Henan scientific research of Chinese Medicine special projects","award":["20-21ZY2153"],"award-info":[{"award-number":["20-21ZY2153"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Ardisiae Japonicae Herba (AJH), the dried whole herb of Ardisia japonica (Thunb.) Blume [Primulaceae], has been used in treating chronic obstructive pulmonary disease (COPD) in China. However, the material basis and molecular mechanisms of AJH against COPD remain unclear. Therefore, in this study, we attempt to establish a systematic approach to elucidate the material basis and molecular mechanisms through compound identification, network analysis, molecular docking, and experimental validation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Ultra-high performance liquid chromatography-Orbitrap Fusion mass spectrometry (UPLC-Orbitrap Fusion MS) was used to characterize the chemical compounds of AJH. The SwissTargetPrediction, String and Metascape databases were selected for network pharmacology analysis, including target prediction, protein-protein interaction (PPI) network analysis, GO and KEGG pathway enrichment analysis. Cytoscape 3.7.2 software was used to construct a component-target-pathway network to screen out the main active compounds. Autodock Vina software was used to verify the affinity between the key compounds and targets. TNF-\u03b1-stimulated A549 cell inflammation model was built to further verify the anti-inflammatory effects of active compounds.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Altogether, 236 compounds were identified in AJH, including 33 flavonoids, 21 Phenylpropanoids, 46 terpenes, 7 quinones, 27 steroids, 71 carboxylic acids and 31 other compounds. Among them, 41 compounds were selected as the key active constituents, which might exhibit therapeutic effects against COPD by modulating 65 corresponding targets primarily involved in inflammation\/metabolism\/immune-related pathways. The results of molecular docking showed that the key compounds could spontaneously bind to the receptor proteins with a strong binding ability. Finally, the anti-inflammatory effects of the three active compounds were validated with the decreased levels of Interleukin-6 (IL-6) and Matrix Metalloproteinase 9 (MMP9) in TNF-\u03b1-induced A549 cells model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study clarified that AJH may exert therapeutic actions for COPD via regulating inflammation\/immune\/metabolism-related pathways using UPLC-Orbitrap Fusion MS technology combined with network pharmacology for the first time. This study had a deeper exploration of the chemical components and pharmacological activities in AJH, which provided a reference for the further study and clinical application of AJH in the treatment of COPD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0269087","type":"journal-article","created":{"date-parts":[[2022,6,15]],"date-time":"2022-06-15T14:05:55Z","timestamp":1655301955000},"page":"e0269087","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Systematic characterization of the effective constituents and molecular mechanisms of Ardisiae Japonicae Herba using UPLC-Orbitrap Fusion MS and network pharmacology"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1830-6230","authenticated-orcid":true,"given":"Suxiang","family":"Feng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongrong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuefang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yange","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiansheng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,6,15]]},"reference":[{"issue":"5","key":"pone.0269087.ref001","doi-asserted-by":"crossref","DOI":"10.1183\/13993003.00164-2019","article-title":"Global Strategy for the Diagnosis, Management, and Prevention of Chronic Obstructive Lung Disease: the GOLD science committee report 2019","volume":"53","author":"D Singh","year":"2019","journal-title":"Eur Respir J"},{"issue":"2","key":"pone.0269087.ref002","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S2213-2600(15)00004-1","article-title":"Burden of asthma and chronic obstructive pulmonary disease and access to essential medicines in low-income and middle-income countries","volume":"3","author":"D Beran","year":"2015","journal-title":"Lancet Respir Med"},{"issue":"1","key":"pone.0269087.ref003","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1164\/rccm.200901-0047OC","article-title":"The natural history of chronic airflow obstruction revisited: an analysis of the Framingham offspring cohort","volume":"180","author":"R Kohansal","year":"2009","journal-title":"Am J Respir Crit Care Med"},{"issue":"7","key":"pone.0269087.ref004","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1164\/rccm.201211-2004OC","article-title":"Associations of ambient air pollution with chronic obstructive pulmonary disease hospitalization and mortality","volume":"187","author":"WQ Gan","year":"2013","journal-title":"Am J Respir Crit Care Med"},{"key":"pone.0269087.ref005","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.2147\/COPD.S134161","article-title":"Genetic polymorphism and chronic obstructive pulmonary disease.","volume":"12","author":"C Yuan","year":"2017","journal-title":"Int J Chron Obstruct Pulmon Dis"},{"issue":"10","key":"pone.0269087.ref006","doi-asserted-by":"crossref","first-page":"e0223952","DOI":"10.1371\/journal.pone.0223952","article-title":"The respiratory virome and exacerbations in patients with chronic obstructive pulmonary disease","volume":"14","author":"AL van Rijn","year":"2019","journal-title":"PLoS One"},{"issue":"03","key":"pone.0269087.ref007","first-page":"305","article-title":"Advances in the role of inflammatory signaling pathways in chronic obstructive pulmonary disease","volume":"49","author":"W Cong","year":"2018","journal-title":"Journal of Shanxi Medical University"},{"issue":"5","key":"pone.0269087.ref008","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1164\/rccm.201701-0218PP","article-title":"Global Strategy for the Diagnosis, Management, and Prevention of Chronic Obstructive Lung Disease 2017 Report. 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