{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T20:28:23Z","timestamp":1793132903437,"version":"4.5.0"},"reference-count":65,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T00:00:00Z","timestamp":1654214400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["Pr\u00eamio CAPES 2015 - 0774\/2017"],"award-info":[{"award-number":["Pr\u00eamio CAPES 2015 - 0774\/2017"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["406402\/2018-3"],"award-info":[{"award-number":["406402\/2018-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ-00524-17"],"award-info":[{"award-number":["APQ-00524-17"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007375","name":"Pr\u00f3-Reitoria de Pesquisa, Universidade Federal de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007375","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    <jats:italic>Staphylococcus pseudintermedius<\/jats:italic>\n                    is a major commensal bacterium of the skin and mucosae of dogs and an opportunistic agent responsible for several clinical infections, such as pyoderma, otitis, and surgical wound infections. The emergence of methicillin-resistant\n                    <jats:italic>S<\/jats:italic>\n                    .\n                    <jats:italic>pseudintermedius<\/jats:italic>\n                    (MRSP) has become a problem of great concern in veterinary and human medicine because it is multidrug resistant (MDR) and can also infect humans. This study aimed to identify the occurrence of\n                    <jats:italic>Staphylococcus<\/jats:italic>\n                    spp. in infected patients and investigate the antimicrobial resistance profiles and molecular structure of MRSP isolates. Samples were obtained from two different veterinary clinics; suggestive colonies were submitted to matrix-assisted laser desorption ionization-time of flight (MALDI-ToF) mass spectrometry and confirmed at the species level by polymerase chain reaction (PCR). Sequencing of the\n                    <jats:italic>16S rRNA<\/jats:italic>\n                    and\n                    <jats:italic>rpoB<\/jats:italic>\n                    genes were used in selected samples that were not identified by MALDI-ToF and by the species-specific PCR. Antimicrobial susceptibility and PCR detection of\n                    <jats:italic>mecA<\/jats:italic>\n                    were performed. MRSP isolates were subjected to multilocus sequence typing. Of all the clinical staphylococci (n = 131), 98 (74.8%) were identified as\n                    <jats:italic>S<\/jats:italic>\n                    .\n                    <jats:italic>pseudintermedius<\/jats:italic>\n                    . Multidrug resistance (resistance to \u22653 classes of antimicrobials) was observed in 63.2% of\n                    <jats:italic>S<\/jats:italic>\n                    .\n                    <jats:italic>pseudintermedius<\/jats:italic>\n                    isolates, and 24.5% of\n                    <jats:italic>S<\/jats:italic>\n                    .\n                    <jats:italic>pseudintermedius<\/jats:italic>\n                    isolates were methicillin-resistant. Half of the MRSP isolates were isolated from surgical site infections. Among the ten sequence types (ST) identified, nine were novel. ST71 was the most prevalent and associated with resistance to fluoroquinolones. Prior antimicrobial therapy, hospitalization, and surgical site infections seemed to be risk factors for MRSP acquisition. The present study showed a high rate of MDR staphylococci in infected dogs. MRSP was isolated from different clinical conditions, mainly surgical site infections. Additionally, this is the first study to extensively investigate the population structure of MRSP in Brazil, which revealed the dispersion of CC71 and nine novel ST. These findings raise concerns for both animal and human health due to the zoonotic potential of this species and limited therapeutic options available for MRSP infections.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0269422","type":"journal-article","created":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T13:42:31Z","timestamp":1654263751000},"page":"e0269422","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":56,"title":["Occurrence and characterization of methicillin-resistant Staphylococcus spp. in diseased dogs in Brazil"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4641-6576","authenticated-orcid":true,"given":"Fl\u00e1via Mello","family":"Viegas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordana Almeida","family":"Santana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brendhal Almeida","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael Gariglio Clark","family":"Xavier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e1udia Teixeira","family":"Bonisson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00falia Lara Sette","family":"C\u00e2mara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rio Cesar","family":"Renn\u00f3","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Luis Reis","family":"Cunha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1022-6842","authenticated-orcid":true,"given":"Henrique C\u00e9sar Pereira","family":"Figueiredo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Carlos Faria","family":"Lobato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0074-2488","authenticated-orcid":true,"given":"Rodrigo Ot\u00e1vio Silveira","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,6,3]]},"reference":[{"issue":"4","key":"pone.0269422.ref001","doi-asserted-by":"crossref","DOI":"10.1111\/j.1365-3164.2012.01046.x","article-title":"Staphylococcus pseudintermedius in the dog: Taxonomy, diagnostics, 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