{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T06:30:32Z","timestamp":1790058632629,"version":"4.0.1"},"reference-count":76,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T00:00:00Z","timestamp":1655942400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Individuals with hyperlipidemia are two times more likely to develop atherosclerotic cardiovascular disease (ASCVD) as opposed to those with controlled serum total cholesterol (TC) levels. Considering the documented adverse events of the current lipid-lowering medications which ultimately affect patient\u2019s compliance, substantial efforts have been made to develop new therapeutic strategies. Probiotics, on the other hand, are reported to have lipid-lowering activity with the added benefit of being generally well-tolerated making it an appealing adjuvant therapy.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>A total of fifty Lactic acid bacteria (LAB) were isolated from raw milk (human and animal) and dairy products. Isolates demonstrating promising <jats:italic>in vitro<\/jats:italic> cholesterol removal capabilities were morphologically and biochemically characterized. Lastly, two bacterial candidates were selected for evaluation of their potential hypolipidemic activity using a laboratory animal model. Statistical differences between the means were analyzed by one-way analysis of variance (ANOVA) followed by Tukey\u2019s post-hoc test. A <jats:italic>p<\/jats:italic>-value &lt; 0.05 was considered statistically significant.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>Most of the isolates demonstrated an <jats:italic>in vitro<\/jats:italic> cholesterol removal activity. The six LAB isolates showing the highest cholesterol removal activity (36.5\u201355.6%) were morphologically and biochemically identified as <jats:italic>Lactobacillus<\/jats:italic>, <jats:italic>Pediococcus<\/jats:italic>, and <jats:italic>Lactococcus<\/jats:italic> species. The results demonstrated two promising antihyperlipidemic candidates, a <jats:italic>Lactococcus lactis<\/jats:italic> ssp. <jats:italic>lactis<\/jats:italic> with an <jats:italic>in vivo<\/jats:italic> significant reduction of serum triglycerides (TG) levels by 34.3%, and a <jats:italic>Pediococcus<\/jats:italic> sp. that was able to significantly reduce both the serum TC and TG levels by 17.3% and 47.0%, respectively, as compared to the diet-induced hyperlipidemic animal group.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>This study further supports the growing evidence regarding the antihyperlipidemic activity among probiotics, presenting them as a promising therapeutic approach for the management of hyperlipidemia.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0269953","type":"journal-article","created":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T17:42:42Z","timestamp":1656006162000},"page":"e0269953","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":17,"title":["Hypolipidemic activity of lactic acid bacteria: Adjunct therapy for potential probiotics"],"prefix":"10.1371","volume":"17","author":[{"given":"Shima Mahmoud","family":"Ali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma E.","family":"Salem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0828-1420","authenticated-orcid":true,"given":"Mohammad M.","family":"Aboulwafa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riham M.","family":"Shawky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,6,23]]},"reference":[{"issue":"10258","key":"pone.0269953.ref001","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1016\/S0140-6736(20)30925-9","article-title":"Global burden of 369 diseases and injuries in 204 countries and territories, 1990\u20132019: a systematic analysis for the Global Burden of Disease Study 2019","volume":"396","author":"C Abbafati","year":"2020","journal-title":"The Lancet [Internet]."},{"issue":"4","key":"pone.0269953.ref002","first-page":"e38","article-title":"Heart Disease and Stroke Statistics\u20142016 Update","volume":"133","author":"D Mozaffarian","year":"2016","journal-title":"Circulation [Internet]."},{"key":"pone.0269953.ref003","first-page":"1","article-title":"Nutrition and skeletal muscle","author":"S Walrand","year":"2018","journal-title":"Nutrition and Skeletal Muscle"},{"key":"pone.0269953.ref004","doi-asserted-by":"crossref","first-page":"6171","DOI":"10.1016\/B0-12-227055-X\/01292-X","article-title":"Whisky, Whiskey, and Bourbon.","author":"JR Piggott","year":"2003","journal-title":"Encyclopedia of Food Sciences and Nutrition"},{"issue":"19","key":"pone.0269953.ref005","doi-asserted-by":"crossref","first-page":"2486","DOI":"10.1001\/jama.285.19.2486","article-title":"Executive Summary of The Third Report of The National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, And Treatment of High Blood Cholesterol In Adults (Adult Treatment Panel III).","volume":"285","author":"JI Cleeman","year":"2001","journal-title":"JAMA [Internet]."},{"issue":"5","key":"pone.0269953.ref006","first-page":"1663","article-title":"G, S B, T M. 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