{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:42:02Z","timestamp":1792802522360,"version":"4.5.0"},"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2022,6,16]],"date-time":"2022-06-16T00:00:00Z","timestamp":1655337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["Sir Henry Dale Fellowship 098436\/Z\/12\/B"],"award-info":[{"award-number":["Sir Henry Dale Fellowship 098436\/Z\/12\/B"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["Sir Henry Dale Fellowship 221805\/Z\/20\/Z"],"award-info":[{"award-number":["Sir Henry Dale Fellowship 221805\/Z\/20\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"publisher","award":["Clinical Research Training Fellowship FS\/17\/18\/32449"],"award-info":[{"award-number":["Clinical Research Training Fellowship FS\/17\/18\/32449"]}],"id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"publisher","award":["Senior Fellowship FS\/19\/18\/34252"],"award-info":[{"award-number":["Senior Fellowship FS\/19\/18\/34252"]}],"id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Oxford British Heart Foundation Centre of Research Excellence","award":["RE\/18\/3\/34214"],"award-info":[{"award-number":["RE\/18\/3\/34214"]}],"id":[{"id":"https:\/\/ror.org\/02wdwnk04","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Oxford NIHR Biomedical Research Centre","award":["BRC-1215-20008"],"award-info":[{"award-number":["BRC-1215-20008"]}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["Schr\u00f6dinger Fellowship J 4043"],"award-info":[{"award-number":["Schr\u00f6dinger Fellowship J 4043"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006109","name":"Vedeck\u00e1 Grantov\u00e1 Agent\u00fara M\u0160VVa\u0160 SR a SAV","doi-asserted-by":"publisher","award":["2\/0003\/20"],"award-info":[{"award-number":["2\/0003\/20"]}],"id":[{"id":"10.13039\/501100006109","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005357","name":"Agent\u00fara na Podporu V\u00fdskumu a V\u00fdvoja","doi-asserted-by":"publisher","award":["19\u20130032"],"award-info":[{"award-number":["19\u20130032"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018956","name":"NIHR Cambridge Biomedical Research Centre","doi-asserted-by":"publisher","award":["BRC-1215-20014"],"award-info":[{"award-number":["BRC-1215-20014"]}],"id":[{"id":"10.13039\/501100018956","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Phosphorus magnetic resonance spectroscopy (\n                    <jats:sup>31<\/jats:sup>\n                    P-MRS) has previously demonstrated decreased energy reserves in the form of phosphocreatine to adenosine-tri-phosphate ratio (PCr\/ATP) in the hearts of patients with type 2 diabetes (T2DM). Recent\n                    <jats:sup>31<\/jats:sup>\n                    P-MRS techniques using 7T systems, e.g. long mixing time stimulated echo acquisition mode (STEAM), allow deeper insight into cardiac metabolism through assessment of inorganic phosphate (Pi) content and myocardial pH, which play pivotal roles in energy production in the heart. Therefore, we aimed to further explore the cardiac metabolic phenotype in T2DM using STEAM at 7T. Seventeen patients with T2DM and twenty-three healthy controls were recruited and their cardiac PCr\/ATP, Pi\/PCr and pH were assessed at 7T. Diastolic function of all patients with T2DM was assessed using echocardiography to investigate the relationship between diastolic dysfunction and cardiac metabolism. Mirroring the decreased PCr\/ATP (1.70\u00b10.31 vs. 2.07\u00b10.39; p&lt;0.01), the cardiac Pi\/PCr was increased (0.13\u00b10.07 vs. 0.10\u00b10.03; p = 0.02) in T2DM patients in comparison to healthy controls. Myocardial pH was not significantly different between the groups (7.14\u00b10.12 vs. 7.10\u00b10.12; p = 0.31). There was a negative correlation between PCr\/ATP and diastolic function (R\n                    <jats:sup>2<\/jats:sup>\n                    = 0.33; p = 0.02) in T2DM. No correlation was observed between diastolic function and Pi\/PCr and (R\n                    <jats:sup>2<\/jats:sup>\n                    = 0.16; p = 0.21). In addition, we did not observe any correlation between cardiac PCr\/ATP and Pi\/PCr (p = 0.19). Using STEAM\n                    <jats:sup>31<\/jats:sup>\n                    P-MRS at 7T we have for the first time explored Pi\/PCr in the diabetic human heart and found it increased when compared to healthy controls. The lack of correlation between measured PCr\/ATP and Pi\/PCr suggests that independent mechanisms might contribute to these perturbations.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0269957","type":"journal-article","created":{"date-parts":[[2022,6,16]],"date-time":"2022-06-16T13:34:57Z","timestamp":1655386497000},"page":"e0269957","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":16,"title":["Increased cardiac Pi\/PCr in the diabetic heart observed using phosphorus magnetic resonance spectroscopy at 7T"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2567-3642","authenticated-orcid":true,"given":"Ladislav","family":"Valkovi\u010d","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Apps","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane","family":"Ellis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Neubauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damian J.","family":"Tyler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albrecht Ingo","family":"Schmid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver J.","family":"Rider","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher T.","family":"Rodgers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,6,16]]},"reference":[{"issue":"1","key":"pone.0269957.ref001","doi-asserted-by":"crossref","first-page":"5","DOI":"10.2337\/diacare.24.1.5","article-title":"Diastolic dysfunction in normotensive men with well-controlled type 2 diabetes: importance of maneuvers in echocardiographic screening for preclinical diabetic cardiomyopathy","volume":"24","author":"P Poirier","year":"2001","journal-title":"Diabetes Care"},{"issue":"6","key":"pone.0269957.ref002","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1016\/0002-9149(72)90595-4","article-title":"New type of cardiomyopathy associated with diabetic glomerulosclerosis","volume":"30","author":"S Rubler","year":"1972","journal-title":"Am J Cardiol"},{"issue":"4","key":"pone.0269957.ref003","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.jacc.2009.12.003","article-title":"The development of heart failure in patients with diabetes mellitus and pre-clinical diastolic dysfunction a population-based study","volume":"55","author":"AM From","year":"2010","journal-title":"J Am Coll Cardiol"},{"issue":"46","key":"pone.0269957.ref004","doi-asserted-by":"crossref","first-page":"3461","DOI":"10.1093\/eurheartj\/ehv442","article-title":"Cardiac energetics, oxygenation, and perfusion during increased workload in patients with type 2 diabetes mellitus","volume":"37","author":"E Levelt","year":"2016","journal-title":"European heart journal"},{"issue":"9","key":"pone.0269957.ref005","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1038\/s41569-020-0339-2","article-title":"Mechanisms of diabetic cardiomyopathy and potential therapeutic strategies: preclinical and clinical evidence","volume":"17","author":"Y Tan","year":"2020","journal-title":"Nat Rev Cardiol"},{"issue":"4","key":"pone.0269957.ref006","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1016\/j.yjmcc.2011.01.007","article-title":"Metabolism, hypoxia and the diabetic heart","volume":"50","author":"LC Heather","year":"2011","journal-title":"J Mol Cell Cardiol"},{"issue":"4","key":"pone.0269957.ref007","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1093\/cvr\/cvx018","article-title":"Metabolic Remodeling in Diabetic Cardiomyopathy","volume":"113","author":"CR Chong","year":"2017","journal-title":"Cardiovasc Res"},{"issue":"6","key":"pone.0269957.ref008","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1161\/CIRCRESAHA.119.316260","article-title":"Noninvasive In Vivo Assessment of Cardiac Metabolism in the Healthy and Diabetic Human Heart Using Hyperpolarized (13)C MRI","volume":"126","author":"OJ Rider","year":"2020","journal-title":"Circ Res"},{"issue":"7285","key":"pone.0269957.ref009","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1016\/S0140-6736(63)91500-9","article-title":"The glucose fatty-acid cycle. 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