{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T15:01:52Z","timestamp":1780066912945,"version":"3.54.0"},"reference-count":44,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2022,9,27]],"date-time":"2022-09-27T00:00:00Z","timestamp":1664236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010198","name":"Ministerio de Econom\u00eda, Industria y Competitividad, Gobierno de Espa\u00f1a","doi-asserted-by":"publisher","award":["BIO2017-83472-R"],"award-info":[{"award-number":["BIO2017-83472-R"]}],"id":[{"id":"10.13039\/501100010198","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n","doi-asserted-by":"publisher","award":["SEV-2016-0672"],"award-info":[{"award-number":["SEV-2016-0672"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The spider mite<jats:italic>Tetranychus urticae<\/jats:italic>is an economically important agricultural pest, which feeds on a broad spectrum of plant species. In an RNAseq experiment performed in our laboratory, 4 of the 15 members of the RIN4-like\/NOI family of<jats:italic>Arabidopsis thaliana<\/jats:italic>were significantly overexpressed after<jats:italic>T<\/jats:italic>.<jats:italic>urticae<\/jats:italic>infestation. Two of them (NOI3 and NOI5) are shorter and harbour one NOI domain, which characterises this family, and the other two (NOI10 and NOI11) have two-NOI domains. The only member of this family characterized is RIN4, a two-NOI intrinsically disordered protein anchored to the plasma membrane and involved in plant defence against bacterial pathogens. The function of all other members of the RIN4-like\/NOI<jats:italic>Arabidopsis<\/jats:italic>family and their putative role in herbivore defence remains unknown. We perform a comparative genomic analysis of RIN4-like\/NOI sequences to study the evolutionary features of this protein family and the distribution of its members among species. We show that short one-NOI proteins were more numerous and exhibited lower disorder propensity compared to two-NOI members. NOI10 and NOI11, from the two-NOI group, are included in a clade-specific expansion of Brassicaceae with unique predicted posttranslational modification sites and clear predicted structural differences from RIN4. Our analysis suggests that the members of the RIN4-like\/NOI family upregulated after mite feeding have novel functions different from those assigned to RIN4, likely involving adaptation to stress specialisation.<\/jats:p>","DOI":"10.1371\/journal.pone.0270791","type":"journal-article","created":{"date-parts":[[2022,9,27]],"date-time":"2022-09-27T17:33:58Z","timestamp":1664300038000},"page":"e0270791","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Comparative and evolutionary analysis of Arabidopsis RIN4-like\/NOI proteins induced by herbivory"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1822-6839","authenticated-orcid":true,"given":"Estefania","family":"Contreras","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7826-5872","authenticated-orcid":true,"given":"Manuel","family":"Martinez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,9,27]]},"reference":[{"issue":"2","key":"pone.0270791.ref001","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1111\/jipb.13026","article-title":"Comparative transcriptomics reveals hidden issues in the plant response to arthropod herbivores","volume":"63","author":"ME Santamaria","year":"2021","journal-title":"J Integr Plant Biol"},{"issue":"1","key":"pone.0270791.ref002","doi-asserted-by":"crossref","first-page":"12996","DOI":"10.1038\/s41598-021-92468-6","article-title":"Disentangling transcriptional responses in plant defense against arthropod herbivores","volume":"11","author":"A Garcia","year":"2021","journal-title":"Sci Rep"},{"issue":"7117","key":"pone.0270791.ref003","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1038\/nature05286","article-title":"The plant immune system","volume":"444","author":"JD Jones","year":"2006","journal-title":"Nature"},{"issue":"7852","key":"pone.0270791.ref004","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1038\/s41586-021-03315-7","article-title":"Mutual potentiation of plant immunity by cell-surface and intracellular receptors","volume":"592","author":"BPM Ngou","year":"2021","journal-title":"Nature"},{"key":"pone.0270791.ref005","doi-asserted-by":"crossref","first-page":"102030","DOI":"10.1016\/j.pbi.2021.102030","article-title":"PTI-ETI crosstalk: an integrative view of plant immunity","volume":"62","author":"M Yuan","year":"2021","journal-title":"Curr Opin Plant Biol"},{"issue":"5","key":"pone.0270791.ref006","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1016\/j.cell.2005.03.025","article-title":"Two Pseudomonas syringae type III effectors inhibit RIN4-regulated basal defense in Arabidopsis","volume":"121","author":"MG Kim","year":"2005","journal-title":"Cell"},{"issue":"17","key":"pone.0270791.ref007","doi-asserted-by":"crossref","first-page":"3955","DOI":"10.1111\/febs.12937","article-title":"The intrinsically disordered structural platform of the plant defence hub protein RPM1-interacting protein 4 provides insights into its mode of action in the host-pathogen interface and evolution of the nitrate-induced domain protein family","volume":"281","author":"X Sun","year":"2014","journal-title":"FEBS J"},{"key":"pone.0270791.ref008","doi-asserted-by":"crossref","first-page":"108229","DOI":"10.1016\/j.abb.2019.108229","article-title":"The functional diversity of structural disorder in plant proteins","volume":"680","author":"AA Covarrubias","year":"2020","journal-title":"Arch Biochem Biophys"},{"issue":"13","key":"pone.0270791.ref009","doi-asserted-by":"crossref","first-page":"6912","DOI":"10.1021\/cr400488d","article-title":"Intrinsic disorder in plant proteins and phytopathogenic bacterial effectors","volume":"114","author":"M Mar\u00edn","year":"2014","journal-title":"Chem Rev"},{"issue":"12","key":"pone.0270791.ref010","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.tibs.2012.08.004","article-title":"Intrinsically disordered proteins: a 10-year recap","volume":"37","author":"P. 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