{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T11:17:30Z","timestamp":1788520650202,"version":"build-2803163510"},"reference-count":52,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T00:00:00Z","timestamp":1657238400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007637","name":"Departamento Administrativo de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["1115-777-57608"],"award-info":[{"award-number":["1115-777-57608"]}],"id":[{"id":"10.13039\/100007637","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01-AI095346"],"award-info":[{"award-number":["R01-AI095346"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Multilocus Sequence Typing has become a useful tool for the study of the genetic diversity and population structure of different organisms. In this study, a MLST approach with seven loci (<jats:italic>CP47<\/jats:italic>,<jats:italic>MS5<\/jats:italic>,<jats:italic>MS9<\/jats:italic>,<jats:italic>MSC6-7<\/jats:italic>,<jats:italic>TP14<\/jats:italic>, and<jats:italic>gp60<\/jats:italic>) was used to analyze the genetic diversity of<jats:italic>Cryptosporidium hominis<\/jats:italic>and<jats:italic>Cryptosporidium parvum<\/jats:italic>isolated from 28 Colombian patients. Five<jats:italic>Cryptosporidium<\/jats:italic>species were identified:<jats:italic>C. hominis<\/jats:italic>,<jats:italic>C. parvum<\/jats:italic>,<jats:italic>Cryptosporidium felis<\/jats:italic>,<jats:italic>Cryptosporidium meleagridis<\/jats:italic>, and<jats:italic>Cryptosporidium suis<\/jats:italic>. Unilocus<jats:italic>gp60<\/jats:italic>analysis identified four allelic families for<jats:italic>C. hominis<\/jats:italic>(Ia, Ib, Id, and Ie) and two for<jats:italic>C. parvum<\/jats:italic>(IIa and IIc). There was polymorphic behavior of all markers evaluated for both<jats:italic>C. hominis<\/jats:italic>and<jats:italic>C. parvum<\/jats:italic>, particularly with the<jats:italic>CP47<\/jats:italic>,<jats:italic>MS5<\/jats:italic>, and<jats:italic>gp60<\/jats:italic>markers. Phylogenetic analysis with consensus sequences (CS) of the markers showed a taxonomic agreement with the results obtained with the<jats:italic>18S rRNA<\/jats:italic>and<jats:italic>gp60<\/jats:italic>gene. Additionally, two monophyletic clades that clustered the species<jats:italic>C. hominis<\/jats:italic>and<jats:italic>C. parvum<\/jats:italic>were detected, with a higher number of subclades within the monophyletic groups compared to those with the<jats:italic>gp60<\/jats:italic>gene. Thirteen MLG were identified for<jats:italic>C. hominis<\/jats:italic>and eight for<jats:italic>C. parvum<\/jats:italic>. Haplotypic and nucleotide diversity were detected, but only the latter was affected by the<jats:italic>gp60<\/jats:italic>exclusion from the CS analysis. The gene fixation index showed an evolutionary closeness between the<jats:italic>C. hominis<\/jats:italic>samples and a less evolutionary closeness and greater sequence divergence in the<jats:italic>C. parvum<\/jats:italic>samples. Data obtained in this work support the implementation of MLST analysis in the study of the genetic diversity of<jats:italic>Cryptosporidium<\/jats:italic>, considering the more detailed information that it provides, which may explain some genetic events that with an unilocus approach could not be established. This is the first multilocus analysis of the intra-specific variability of<jats:italic>Cryptosporidium<\/jats:italic>from humans in South America.<\/jats:p>","DOI":"10.1371\/journal.pone.0270995","type":"journal-article","created":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T17:44:28Z","timestamp":1657302268000},"page":"e0270995","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["Multilocus Sequence Typing helps understand the genetic diversity of Cryptosporidium hominis and Cryptosporidium parvum isolated from Colombian patients"],"prefix":"10.1371","volume":"17","author":[{"given":"Johanna","family":"Uran-Velasquez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan F.","family":"Alzate","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana 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