{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T07:08:56Z","timestamp":1778310536027,"version":"3.51.4"},"reference-count":23,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2023,2,3]],"date-time":"2023-02-03T00:00:00Z","timestamp":1675382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100001209","name":"Knights Templar Eye Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001209","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The Fund for Ophthalmic Knowledge"},{"name":"P30 Cancer Center Support Grant","award":["P30 CA008748"],"award-info":[{"award-number":["P30 CA008748"]}]},{"name":"Perry's Promise Fund"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Cell free DNA (<jats:underline>cf<\/jats:underline>DNA) and circulating tumor cell free DNA (<jats:underline>ct<\/jats:underline>DNA) from blood (plasma) are increasingly being used in oncology for diagnosis, monitoring response, identifying cancer causing mutations and detecting recurrences. Circulating tumor <jats:italic>RB1<\/jats:italic> DNA (ctDNA) is found in the blood (plasma) of retinoblastoma patients at diagnosis before instituting treatment (na\u00efve). We investigated ctDNA in na\u00efve unilateral patients before enucleation and during enucleation (6 patients\/ 8 mutations with specimens collected 5\u201340 minutes from severing the optic nerve) In our cohort, following transection the optic nerve, ctDNA <jats:italic>RB1<\/jats:italic> VAF was measurably lower than pre-enucleation levels within five minutes, 50% less within 15 minutes and 90% less by 40 minutes.<\/jats:p>","DOI":"10.1371\/journal.pone.0271505","type":"journal-article","created":{"date-parts":[[2023,2,3]],"date-time":"2023-02-03T18:31:01Z","timestamp":1675449061000},"page":"e0271505","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Mutant-RB1 circulating tumor DNA in the blood of unilateral retinoblastoma patients: What happens during enucleation surgery: A pilot study"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0118-6391","authenticated-orcid":true,"given":"David H.","family":"Abramson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana L.","family":"Mandelker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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