{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T22:49:21Z","timestamp":1768690161915,"version":"3.49.0"},"reference-count":45,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2022,7,21]],"date-time":"2022-07-21T00:00:00Z","timestamp":1658361600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"name":"USDA-ARS, National Animal Disease Center"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The objective was to determine differences in microRNAs (miRNAs) counts in several tissues of calves challenged with<jats:italic>Mycoplasma bovis<\/jats:italic>(<jats:italic>M<\/jats:italic>.<jats:italic>bovis<\/jats:italic>) or with<jats:italic>M<\/jats:italic>.<jats:italic>bovis<\/jats:italic>and bovine viral diarrhea virus (BVDV). Eight calves approximately 2 months of age were randomly assigned to three groups: Control (CT; n = 2),<jats:italic>M<\/jats:italic>.<jats:italic>bovis<\/jats:italic>(MB; n = 3), and Coinfection (CO; n = 3). On day 0, calves in CO were intranasally challenged with BVDV and calves in MB with<jats:italic>M<\/jats:italic>.<jats:italic>bovis<\/jats:italic>. On day 6, CO calves were challenged with<jats:italic>M<\/jats:italic>.<jats:italic>bovis<\/jats:italic>. Calves were euthanized 17 days post-challenge and serum (SER), white blood cells (WBC), liver (LIV), mesenteric (MLN) and tracheal-bronchial (TBLN) lymph nodes, spleen (SPL), and thymus (THY), were collected at necropsy. MiRNAs were extracted from each tissue from each calf. Significant (P&lt; 0.01) differences in miRNAs expression were observed in SER, LIV, MLN, TBLN, SPL, and THY. There were no significant (P&gt; 0.05) miRNAs in WBC. In SER, the CO group had levels of miR-1343-3p significantly higher than the CT and MB groups (P = 0.0071). In LIV and SPL, the CO group had the lowest counts for all significant miRNAs compared to CT and MB. In TBLN, the CT group had the highest counts of miRNAs, compared to MB and CO, in 14 of the 21 significant miRNAs. In THY, the CO group had the highest counts, in 4 of the 6 significant miRNAs compared to CT and MB. BVDV was associated with reduction of miRNAs in LIV, SPL, MLN, and TBLN, and<jats:italic>M<\/jats:italic>.<jats:italic>bovis<\/jats:italic>reduced counts of miRNAs in only TBLN. Measuring circulating miRNAs to assess disease condition or to develop intervention strategies to minimize respiratory diseases in cattle caused by BVDV or<jats:italic>M<\/jats:italic>.<jats:italic>bovis<\/jats:italic>will be of limited use unless an alternative approach is developed to use them as indicators of disease.<\/jats:p>","DOI":"10.1371\/journal.pone.0271581","type":"journal-article","created":{"date-parts":[[2022,7,21]],"date-time":"2022-07-21T17:59:45Z","timestamp":1658426385000},"page":"e0271581","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["MicroRNA profiles for different tissues from calves challenged with Mycoplasma bovis or challenged with Mycoplasma bovis and bovine viral diarrhea virus"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3773-4035","authenticated-orcid":true,"given":"Eduardo","family":"Casas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shollie M.","family":"Falkenberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rohana P.","family":"Dassanayake","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen B.","family":"Register","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John D.","family":"Neill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2022,7,21]]},"reference":[{"issue":"3","key":"pone.0271581.ref001","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/S0749-0720(15)30302-9","article-title":"Economic impact associated with respiratory disease in beef cattle.","volume":"13","author":"D. 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