{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T13:57:06Z","timestamp":1791899826473,"version":"4.3.4"},"reference-count":36,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2022,11,17]],"date-time":"2022-11-17T00:00:00Z","timestamp":1668643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background and aim<\/jats:title>\n                    <jats:p>\n                      Multidrug resistant\n                      <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                      is associated with nosocomial infections in both outbreak and non-outbreak situations. The study intends to evaluate the potential of enterobacterial repetitive intergenic consensus- polymerase chain reaction (ERIC-PCR), a genomic based typing and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) proteomic-based typing techniques for clonal relatedness among multidrug resistant\n                      <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                      isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>\n                      Multidrug resistant clinical isolates of\n                      <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                      (\n                      <jats:italic>n<\/jats:italic>\n                      = 137) were collected from March 2019 to February 2020. Identification and protein-based phylogenetic analysis were performed by MALDI-TOF MS. Genomic typing was done by ERIC-PCR and analyzed by an online data analysis service (PyElph). Dice method with unweighted pair group method with arithmetic mean (UPGMA) program was used to compare the ERIC profiles. The samples were also evaluated by PCR for the presence of genes encoding carbapenemases, extended spectrum beta lactamases (ESBLs) and mobile colistin resistance-1 (\n                      <jats:italic>mcr1<\/jats:italic>\n                      ).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Result and conclusion<\/jats:title>\n                    <jats:p>\n                      The study presents ERIC-PCR as more robust and better discriminatory typing tool in comparison to MALDI-TOF for clonal relatedness in multidrug resistant\n                      <jats:italic>K<\/jats:italic>\n                      .\n                      <jats:italic>pneumoniae<\/jats:italic>\n                      clinical isolates. Isolates were typed into 40 ERIC types, and six groups by MALDI-TOF-MS. PCR-based analysis revealed that all the strains harbored two or more ESBL and carbapenemase genes. None of the isolates revealed the presence of the plasmid mediated\n                      <jats:italic>mcr-1<\/jats:italic>\n                      gene for colistin resistance.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0271652","type":"journal-article","created":{"date-parts":[[2022,11,17]],"date-time":"2022-11-17T13:43:26Z","timestamp":1668692606000},"page":"e0271652","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":19,"title":["Evaluation of ERIC-PCR and MALDI-TOF as typing tools for multidrug resistant Klebsiella pneumoniae clinical isolates from a tertiary care center in India"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5995-3426","authenticated-orcid":true,"given":"Jyoti","family":"Kundu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shubhangi","family":"Kansal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivali","family":"Rathore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meenakshi","family":"Kaundal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9051-3007","authenticated-orcid":true,"given":"Archana","family":"Angrup","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manisha","family":"Biswal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamini","family":"Walia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3369-2640","authenticated-orcid":true,"given":"Pallab","family":"Ray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,11,17]]},"reference":[{"issue":"3","key":"pone.0271652.ref001","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1093\/femsre\/fux013","article-title":"Klebsiella pneumoniae: a major worldwide source and shuttle for antibiotic resistance","volume":"41","author":"S Navon-Venezia","journal-title":"FEMS Microbiology Reviews"},{"issue":"3","key":"pone.0271652.ref002","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1128\/AAC.01169-07","article-title":"Clinical and economic impact of common multidrug-resistant gram-negative bacilli","volume":"52","author":"CG Giske","year":"2008","journal-title":"Antimicrob Agents Chemother"},{"issue":"4","key":"pone.0271652.ref003","doi-asserted-by":"crossref","first-page":"eGS4444","DOI":"10.31744\/einstein_journal\/2019GS4444","article-title":"Economic burden of inpatients infected with Klebsiella pneumoniae carbapenemase","volume":"17","author":"WMD Santos","year":"2019","journal-title":"Einstein (Sao Paulo)."},{"issue":"Issue suppl_2","key":"pone.0271652.ref004","doi-asserted-by":"crossref","first-page":"S225","DOI":"10.1093\/cid\/ciy642","article-title":"In-hospital Medical Costs of Infections Caused by Carbapenem-resistant Klebsiella pneumoniae","volume":"67","author":"W Huang","year":"2018","journal-title":"Clinical Infectious Diseases"},{"issue":"8","key":"pone.0271652.ref005","doi-asserted-by":"crossref","first-page":"514","DOI":"10.3390\/antibiotics9080514","article-title":"Clinical and Economic Burden of Carbapenem-Resistant Infection or Colonization Caused by Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii: A Multicenter Study in China","volume":"9","author":"X Zhen","year":"2020","journal-title":"Antibiotics"},{"key":"pone.0271652.ref006","unstructured":"Bank 2017, W. 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