{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T19:16:48Z","timestamp":1783711008688,"version":"3.55.0"},"reference-count":50,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T00:00:00Z","timestamp":1659398400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006668","name":"Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT 2016 0870"],"award-info":[{"award-number":["PICT 2016 0870"]}],"id":[{"id":"10.13039\/501100006668","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006668","name":"Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT 2018 1681"],"award-info":[{"award-number":["PICT 2018 1681"]}],"id":[{"id":"10.13039\/501100006668","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Sperm selection for assisted reproduction techniques is generally based on basic parameters, while key aspects of sperm competence and its journey from the deposition site to the fertilization site are overlooked. Consequently, identifying molecular markers in spermatozoa that can efficiently predict the fertility of a semen sample could be of great interest, particularly in cases of idiopathic male infertility. When spermatozoa reach the female reproductive tract, it provides to them the cellular and molecular microenvironment needed to acquire fertilizing ability. In this sense, considering the role that integrin \u03b15\u03b21 of spermatozoa plays in reproduction-related events, we investigated the correlation between the subcellular localization of sperm integrin \u03b15\u03b21 and early embryo development outcome after<jats:italic>in vitro<\/jats:italic>fertilization (IVF) procedures in human. Twenty-four semen samples from normozoospermic men and metaphase II (MII) oocytes from healthy women aged under 38 years, from couples who underwent IVF cycles, were used in this work. Sperm \u03b15\u03b21 localization was evaluated by immunofluorescence assay using an antibody against integrin \u03b15 subunit. Integrin \u03b15\u03b21 was mainly localized in the sperm acrosomal region (45.33\u00b17.89%) or the equatorial segment (30.12\u00b17.43%). The early embryo development rate (data obtained from the Fertility Center) correlated positively with the localization of \u03b15\u03b21 in the acrosomal region (number of usable embryos \/ inseminated oocytes: \u03c1 = 0.75; p&lt;0.01 and number of usable embryos\/total number of two pronuclear zygotes: \u03c1 = 0.80; p&lt;0.01). However, this correlation was not significant when the equatorial segment mark was evaluated. In addition, human sperm released from co-culture with bovine oviductal epithelial cells (BOEC) showed a significant enrichment in the acrosomal localization pattern of \u03b15\u03b21 compared to those sperm that were not co-cultured with BOEC (85.20\u00b15.35% vs 35.00\u00b117.09%, respectively, p&lt;0.05). In conclusion, the evaluation of sperm integrin \u03b15\u03b21 immunolocalization could be a useful tool to select sperm with fertilizing ability from human semen samples before IVF procedures.<\/jats:p>","DOI":"10.1371\/journal.pone.0271729","type":"journal-article","created":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T19:04:40Z","timestamp":1659467080000},"page":"e0271729","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evaluation of sperm integrin \u03b15\u03b21 as a potential marker of fertility in humans"],"prefix":"10.1371","volume":"17","author":[{"given":"Zoilo Jos\u00e9","family":"Vernaz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel Mar\u00eda","family":"Lottero-Leconte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Agust\u00edn Isidro","family":"Alonso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sof\u00eda","family":"Rio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maia Florencia","family":"Morales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila","family":"Arroyo-Salvo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla C.","family":"Valiente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Lovaglio Diez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Eugenia","family":"Bogetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Arenas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gast\u00f3n","family":"Rey-Valzacchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6175-0863","authenticated-orcid":true,"given":"Silvina","family":"Perez-Martinez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,8,2]]},"reference":[{"key":"pone.0271729.ref001","doi-asserted-by":"crossref","first-page":"F93","DOI":"10.1530\/REP-17-0374","article-title":"Intracytoplasmic sperm injection: state of the art in humans","volume":"154","author":"GD Palermo","year":"2017","journal-title":"Reproduction"},{"key":"pone.0271729.ref002","unstructured":"WHO. 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