{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:14:23Z","timestamp":1790709263711,"version":"4.1.0"},"reference-count":28,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2022,9,2]],"date-time":"2022-09-02T00:00:00Z","timestamp":1662076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000030","name":"Centers for Disease Control and Prevention","doi-asserted-by":"publisher","award":["BAA 2017-OADS-01"],"award-info":[{"award-number":["BAA 2017-OADS-01"]}],"id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000030","name":"Centers for Disease Control and Prevention","doi-asserted-by":"publisher","award":["BAA 2017-OADS-01"],"award-info":[{"award-number":["BAA 2017-OADS-01"]}],"id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000030","name":"Centers for Disease Control and Prevention","doi-asserted-by":"publisher","award":["BAA 2017-OADS-01"],"award-info":[{"award-number":["BAA 2017-OADS-01"]}],"id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ohio Department of Natural Resources, Division of Wildlife"},{"name":"Ohio Biodiversity Conservation Partnership"},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN\/04800-2017"],"award-info":[{"award-number":["RGPIN\/04800-2017"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Canada Foundation for Innovation\/John R. Evans Leaders Fund grant","award":["35318"],"award-info":[{"award-number":["35318"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Surface waters, especially those receiving wastewater flows, can disseminate antimicrobial resistant bacteria (ARB), antimicrobial resistance genes (ARG), and antibiotics. In the Scioto River of central Ohio, United States, we evaluated fishes as potential sentinels of ARB and antimicrobial contamination and investigated the influence of antimicrobial exposure on the fish intestinal resistome. Seventy-seven fish were collected from river reaches receiving inputs from two wastewater treatment plants that serve the greater Columbus Metropolitan Area. Fish were screened for the presence of cephalosporin-resistant (CeRO) and carbapenem-resistant (CRO) organisms, epidemic carbapenemase genes, and antibiotic drugs and metabolites using culture methods, droplet digital PCR, and ultra-high performance liquid chromatography tandem mass spectroscopy (UHPLC-MS\/MS). Nearly 21% of fish harbored a CeRO in their resistome, with 19.4% exhibiting bacteria expressing an AmpC genotype encoded by <jats:italic>bla<\/jats:italic><jats:sub>CMY<\/jats:sub>, and 7.7% with bacteria expressing an extended-spectrum \u03b2-lactamase phenotype encoded by <jats:italic>bla<\/jats:italic><jats:sub>CTX-M.<\/jats:sub> <jats:italic>bla<\/jats:italic><jats:sub>KPC<\/jats:sub> and <jats:italic>bla<\/jats:italic><jats:sub>NDM<\/jats:sub> were present in 87.7% (57\/65) and 80.4% (37\/46) of the intestinal samples at an average abundance of 10<jats:sup>4<\/jats:sup> copies. Three antibiotics\u2013lincomycin (19.5%), azithromycin (31.2%) and sulfamethoxazole (3.9%)\u2013were found in hepatic samples at average concentrations between 25\u201331 ng\/g. Fish harboring <jats:italic>bla<\/jats:italic><jats:sub>CTX-M<\/jats:sub> and those exposed to azithromycin were at greater odds of being downstream of a wastewater treatment plant. Fish that bioconcentrated antibiotics in their liver were not at greater odds of harboring CeRO, CRO, or epidemic carbapenemase gene copies in their resistome. Our findings confirm that fishes can be effective bioindicators of surface waters contaminated with ARB, ARG, and antibiotics. Moreover, our findings highlight the varying importance of different mechanisms that facilitate establishment of ARB in aquatic ecosystems.<\/jats:p>","DOI":"10.1371\/journal.pone.0272806","type":"journal-article","created":{"date-parts":[[2022,9,2]],"date-time":"2022-09-02T17:26:10Z","timestamp":1662139570000},"page":"e0272806","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":16,"title":["Fish as sentinels of antimicrobial resistant bacteria, epidemic carbapenemase genes, and antibiotics in surface water"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0050-6154","authenticated-orcid":true,"given":"Gregory A.","family":"Ballash","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anca","family":"Baesu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seungjun","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Molly C.","family":"Mills","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dixie F.","family":"Mollenkopf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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