{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T15:01:11Z","timestamp":1790089271665,"version":"4.0.1"},"reference-count":35,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2022,8,11]],"date-time":"2022-08-11T00:00:00Z","timestamp":1660176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003830","name":"Huvepharma","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003830","id-type":"DOI","asserted-by":"publisher"}]},{"name":"E\u00f6tv\u00f6s Lor\u00e1nd Research Network","award":["SA-27\/2021"],"award-info":[{"award-number":["SA-27\/2021"]}]},{"DOI":"10.13039\/501100018818","name":"National Research, Development and Innovation Office","doi-asserted-by":"publisher","award":["\u00daNKP-21-3"],"award-info":[{"award-number":["\u00daNKP-21-3"]}],"id":[{"id":"10.13039\/501100018818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018818","name":"National Research, Development and Innovation Office","doi-asserted-by":"publisher","award":["KDP-2020"],"award-info":[{"award-number":["KDP-2020"]}],"id":[{"id":"10.13039\/501100018818","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p><jats:italic>Mycoplasma hyorhinis<\/jats:italic> is an emerging swine pathogen bacterium causing polyserositis and polyarthritis in weaners and finishers. The pathogen is distributed world-wide, generating significant economic losses. No commercially available vaccine is available in Europe. Therefore, besides improving the housing conditions for prevention, antimicrobial therapy of the diseased animals is the only option to control the infection. Our aim was to determine the minimal inhibitory concentrations (MIC) of ten antimicrobials potentially used against <jats:italic>M<\/jats:italic>. <jats:italic>hyorhinis<\/jats:italic> infection. The antibiotic susceptibility of 76 <jats:italic>M<\/jats:italic>. <jats:italic>hyorhinis<\/jats:italic> isolates from Belgium, Germany, Hungary, Italy and Poland collected between 2019 and 2021 was determined by broth micro-dilution method and mismatch amplification mutation assay (MAMA). Low concentrations of tiamulin (MIC<jats:sub>90<\/jats:sub> 0.312 \u03bcg\/ml), doxycycline (MIC<jats:sub>90<\/jats:sub> 0.078 \u03bcg\/ml), oxytetracycline (MIC<jats:sub>90<\/jats:sub> 0.25 \u03bcg\/ml), florfenicol (MIC<jats:sub>90<\/jats:sub> 2 \u03bcg\/ml) and moderate concentrations of enrofloxacin (MIC<jats:sub>90<\/jats:sub> 1.25 \u03bcg\/ml) inhibited the growth of the isolates. For the tested macrolides and lincomycin, a bimodal MIC pattern was observed (MIC<jats:sub>90<\/jats:sub> &gt;64 \u03bcg\/ml for lincomycin, tulathromycin, tylosin and tilmicosin and 5 \u03bcg\/ml for tylvalosin). The results of the MAMA assay were in line with the conventional method with three exceptions. Based on our statistical analyses, significant differences in MIC values of tiamulin and doxycycline were observed between certain countries. Our results show various levels of antimicrobial susceptibility among <jats:italic>M<\/jats:italic>. <jats:italic>hyorhinis<\/jats:italic> isolates to the tested antibiotics. The data underline the importance of susceptibility monitoring on pan-European level and provides essential information for proper antibiotic choice in therapy.<\/jats:p>","DOI":"10.1371\/journal.pone.0272903","type":"journal-article","created":{"date-parts":[[2022,8,11]],"date-time":"2022-08-11T17:36:04Z","timestamp":1660239364000},"page":"e0272903","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["Antimicrobial susceptibility profiles of Mycoplasma hyorhinis strains isolated from five European countries between 2019 and 2021"],"prefix":"10.1371","volume":"17","author":[{"given":"Ulrich","family":"Klein","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorottya","family":"F\u00f6ldi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolett","family":"Belecz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"Hrivn\u00e1k","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zolt\u00e1n","family":"Somogyi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"Gastaldelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianna","family":"Merenda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3258-172X","authenticated-orcid":true,"given":"Salvatore","family":"Catania","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0270-2914","authenticated-orcid":true,"given":"Arkadiusz","family":"Dors","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ute","family":"Siesenop","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Vyt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zsuzsa","family":"Kreizinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wouter","family":"Depondt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5698-5713","authenticated-orcid":true,"given":"Mikl\u00f3s","family":"Gyuranecz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,8,11]]},"reference":[{"key":"pone.0272903.ref001","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1136\/vr.104064","article-title":"Infection dynamics of Mycoplasma hyorhinis in three commercial pig populations","volume":"181","author":"MJ Clavijo","year":"2017","journal-title":"Veterinary Record"},{"key":"pone.0272903.ref002","doi-asserted-by":"crossref","first-page":"e0223653","DOI":"10.1371\/journal.pone.0223653","article-title":"Molecular epidemiology of Mycoplasma hyorhinis porcine field isolates in the United States","volume":"14","author":"MJ Clavijo","year":"2019","journal-title":"PLoS ONE"},{"key":"pone.0272903.ref003","doi-asserted-by":"crossref","first-page":"e0209975","DOI":"10.1371\/journal.pone.0209975","article-title":"Mycoplasma hyorhinis and Mycoplasma hyosynoviae dual detection patterns in dams and piglets","volume":"14","author":"LR Roos","year":"2019","journal-title":"PLoS ONE"},{"key":"pone.0272903.ref004","doi-asserted-by":"crossref","unstructured":"Pieters MG, Maes D. Mycoplasmosis. In: Zimmerman JJ, Karriker LA, Ramirez A, Schwartz KJ, Stevenson GW, Zhang J, editors. Diseases of Swine. Wiley; 2019. pp. 863\u201383.","DOI":"10.1002\/9781119350927.ch56"},{"key":"pone.0272903.ref005","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1093\/jac\/40.5.622","article-title":"Antibiotic susceptibilities of mycoplasmas and treatment of mycoplasmal infections","volume":"40","author":"D. Taylor-Robinson","year":"1997","journal-title":"Journal of Antimicrobial Chemotherapy"},{"key":"pone.0272903.ref006","doi-asserted-by":"crossref","first-page":"105474","DOI":"10.1016\/j.tvjl.2020.105474","article-title":"Antimicrobial treatment of Mycoplasma hyopneumoniae infections","volume":"259\u2013260","author":"D Maes","year":"2020","journal-title":"The Veterinary Journal"},{"key":"pone.0272903.ref007","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1292\/jvms.67.795","article-title":"Macrolides and Lincomycin Susceptibility of Mycoplasma hyorhinis and Variable Mutation of Domain II and V in 23S Ribosomal RNA","volume":"67","author":"H Kobayashi","year":"2005","journal-title":"J Vet Med Sci"},{"key":"pone.0272903.ref008","doi-asserted-by":"crossref","first-page":"1030","DOI":"10.1128\/AAC.40.4.1030","article-title":"Macrolide susceptibility of Mycoplasma hyorhinis isolated from piglets","volume":"40","author":"H Kobayashi","year":"1996","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0272903.ref009","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.vetmic.2018.11.027","article-title":"Antibiotic susceptibility profiles of Mycoplasma hyorhinis strains isolated from swine in Hungary","volume":"228","author":"K Bek\u0151","year":"2019","journal-title":"Veterinary Microbiology"},{"key":"pone.0272903.ref010","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1556\/004.2021.00026","article-title":"Development of a molecular biological assay for the detection of markers related to decreased susceptibility to macrolides and lincomycin in Mycoplasma hyorhinis","volume":"69","author":"D F\u00f6ldi","year":"2021","journal-title":"Acta Vet Hung"},{"key":"pone.0272903.ref011","first-page":"405","article-title":"Establishment and application of a real-time, duplex PCR method for simultaneous detection of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis","volume":"25","author":"Y Wu","year":"2019","journal-title":"Kafkas Univ Vet Fak Derg"},{"key":"pone.0272903.ref012","first-page":"12","article-title":"Development and optimization of a cell-associated challenge model for Mycoplasma hyorhinis in 7-week-old cesarean-derived, colostrum-deprived pigs","volume":"82","author":"B Martinson","year":"2018","journal-title":"The Canadian Journal of Veterinary Research"},{"key":"pone.0272903.ref013","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1007\/s11259-005-1866-3","article-title":"The Occurrence of Mycoplasmas in the Lungs of Swine in Gran Canaria (Spain)","volume":"29","author":"P Assun\u00e7a\u00f5","year":"2005","journal-title":"Vet Res Commun"},{"key":"pone.0272903.ref014","doi-asserted-by":"crossref","first-page":"804","DOI":"10.2307\/1592417","article-title":"Avian Mycoplasma Identification Using Polymerase Chain Reaction Amplicon and Restriction Fragment Length Polymorphism Analysis","volume":"39","author":"LH Lauerman","year":"1995","journal-title":"Avian Diseases"},{"key":"pone.0272903.ref015","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1051\/vetres:2000100","article-title":"Guidelines and recommendations for antimicrobial minimum inhibitory concentration (MIC) testing against veterinary mycoplasma species","volume":"31","author":"PCT Hannan","year":"2000","journal-title":"Vet Res"},{"key":"pone.0272903.ref016","volume-title":"R: A Language and Environment for Statistical Computing","author":"R Core Team","year":"2021"},{"issue":"8","key":"pone.0272903.ref017","doi-asserted-by":"crossref","DOI":"10.18637\/jss.v028.i08","article-title":"Implementing a Class of Permutation Tests: The coin Package","volume":"28","author":"T Hothorn","year":"2008","journal-title":"J Stat Soft"},{"key":"pone.0272903.ref018","unstructured":"Christensen RHB. ordinal\u2014Regression Models for Ordinal Data. 2019. https:\/\/CRAN.R-project.org\/package\/ordinal"},{"key":"pone.0272903.ref019","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1128\/AAC.35.2.228","article-title":"Comparison of methods for in vitro testing of susceptibility of porcine Mycoplasma species to antimicrobial agents","volume":"35","author":"EA Ter Laak","year":"1991","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0272903.ref020","doi-asserted-by":"crossref","first-page":"2037","DOI":"10.1128\/AAC.41.9.2037","article-title":"Comparative susceptibilities of various animal-pathogenic mycoplasmas to fluoroquinolones","volume":"41","author":"PC Hannan","year":"1997","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0272903.ref021","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1093\/jac\/dku347","article-title":"Assigning defined daily doses animal: a European multi-country experience for antimicrobial products authorized for usage in pigs","volume":"70","author":"M Postma","year":"2015","journal-title":"Journal of Antimicrobial Chemotherapy"},{"key":"pone.0272903.ref022","doi-asserted-by":"crossref","DOI":"10.1128\/microbiolspec.ARBA-0030-2018","article-title":"Antimicrobial Resistance in Mycoplasma spp","volume":"6","author":"AV Gautier-Bouchardon","year":"2018","journal-title":"Microbiol Spectrum"},{"key":"pone.0272903.ref023","doi-asserted-by":"crossref","first-page":"1716","DOI":"10.1292\/jvms.17-0279","article-title":"Biofilm formation and determination of minimum biofilm eradication concentration of antibiotics in Mycoplasma hyopneumoniae","volume":"79","author":"DD Tassew","year":"2017","journal-title":"The Journal of Veterinary Medical Science"},{"key":"pone.0272903.ref024","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1046\/j.1365-2885.1998.00131.x","article-title":"Bioavailability of spiramycin and lincomycin after oral administration to fed and fasted pigs","volume":"21","author":"P Nielsen","year":"1998","journal-title":"Journal of Veterinary Pharmacology and Therapeutics"},{"key":"pone.0272903.ref025","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1046\/j.1365-2885.2001.00342.x","article-title":"Disposition of oxytetracycline in pigs after i.m. administration of two long-acting formulations","volume":"24","author":"G El Korchi","year":"2001","journal-title":"J Vet Pharmacol Ther"},{"key":"pone.0272903.ref026","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1111\/j.1365-2885.2005.00700.x","article-title":"PK and PK\/PD of doxycycline in drinking water after therapeutic use in pigs","volume":"28","author":"C Prats","year":"2005","journal-title":"J Vet Pharmacol Ther"},{"key":"pone.0272903.ref027","doi-asserted-by":"crossref","unstructured":"Gigu\u00e8re S. Lincosamides, Pleuromutilins, and Streptogramins. In: Gigu\u00e8re S, Prescott JF, Dowling PM, editors. Antimicrobial Therapy in Veterinary Medicine. Wiley; 2013. pp. 199\u2013210.","DOI":"10.1002\/9781118675014.ch12"},{"key":"pone.0272903.ref028","doi-asserted-by":"crossref","first-page":"1823","DOI":"10.1080\/19440049.2015.1078915","article-title":"Pharmacokinetics of tulathromycin in edible tissues of healthy and experimentally infected pigs with Actinobacillus pleuropneumoniae","volume":"32","author":"T Bladek","year":"2015","journal-title":"Food Additives & Contaminants: Part A"},{"key":"pone.0272903.ref029","doi-asserted-by":"crossref","first-page":"e000079","DOI":"10.1136\/vetreco-2014-000079","article-title":"Use of tylvalosin in the control of porcine enzootic pneumonia","volume":"2","author":"FJ Pallar\u00e9s","year":"2015","journal-title":"Vet rec open"},{"key":"pone.0272903.ref030","first-page":"36","article-title":"Pharmacokinetic and Pharmacodynamic Integration and Modeling of Enrofloxacin in Swine for Escherichia coli","volume":"7","author":"J Wang","year":"2016","journal-title":"Front Microbiol"},{"key":"pone.0272903.ref031","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1186\/s12917-018-1645-3","article-title":"Pharmacokinetic-pharmacodynamic modeling of tylosin against Streptococcus suis in pigs","volume":"14","author":"L Huang","year":"2018","journal-title":"BMC Vet Res"},{"key":"pone.0272903.ref032","doi-asserted-by":"crossref","first-page":"2","DOI":"10.3389\/fphar.2018.00002","article-title":"PK-PD Integration Modeling and Cutoff Value of Florfenicol against Streptococcus suis in Pigs","volume":"9","author":"Z Lei","year":"2018","journal-title":"Front Pharmacol"},{"key":"pone.0272903.ref033","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1080\/00480169.2019.1633434","article-title":"Comparison of pharmacokinetics of tilmicosin in healthy pigs and pigs experimentally infected with Actinobacillus pleuropneumoniae","volume":"67","author":"J Xiong","year":"2019","journal-title":"New Zealand Veterinary Journal"},{"key":"pone.0272903.ref034","unstructured":"Klein U, Gyuranecz M, Catania S, Claerhout L, Depondt W. PK\/PD and clinical relationships of Vetmulin\u00ae Injection (tiamulin base) administered to pigs for the treatment of Mycoplasmal arthritis. Poster: ESPHM Congress; 11\u201313 May 2022; Budapest."},{"key":"pone.0272903.ref035","doi-asserted-by":"crossref","first-page":"109","DOI":"10.3390\/pharmaceutics14010109","article-title":"Synovial and Systemic Pharmacokinetics of Florfenicol and PK\/PD Integration against Streptococcus suis in Pigs","volume":"14","author":"Z Somogyi","year":"2022","journal-title":"Pharmaceutics"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0272903","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,11]],"date-time":"2022-08-11T17:36:36Z","timestamp":1660239396000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0272903"}},"subtitle":[],"editor":[{"given":"Chih-Horng","family":"Kuo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,8,11]]},"references-count":35,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,8,11]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0272903","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,11]]}}}