{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T08:33:06Z","timestamp":1772785986119,"version":"3.50.1"},"reference-count":71,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2022,8,18]],"date-time":"2022-08-18T00:00:00Z","timestamp":1660780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p><jats:italic>Streptococcus pneumoniae (S<\/jats:italic>. <jats:italic>pneumoniae)<\/jats:italic>, the major etiological agent of community-acquired pneumonia (CAP) contributes significantly to the global burden of infectious diseases which is getting resistant day by day. Nearly 30% of the <jats:italic>S<\/jats:italic>. <jats:italic>pneumoniae<\/jats:italic> genomes encode hypothetical proteins (HPs), and better understandings of these HPs in virulence and pathogenicity plausibly decipher new treatments. Some of the HPs are present across many <jats:italic>Streptococcus<\/jats:italic> species, systematic assessment of these unexplored HPs will disclose prospective drug targets. In this study, through a stringent bioinformatics analysis of the core genome and proteome of <jats:italic>S<\/jats:italic>. <jats:italic>pneumoniae<\/jats:italic> PCS8235, we identified and analyzed 28 HPs that are common in many <jats:italic>Streptococcus<\/jats:italic> species and might have a potential role in the virulence or pathogenesis of the bacteria. Functional annotations of the proteins were conducted based on the physicochemical properties, subcellular localization, virulence prediction, protein-protein interactions, and identification of essential genes, to find potentially druggable proteins among 28 HPs. The majority of the HPs are involved in bacterial transcription and translation. Besides, some of them were homologs of enzymes, binding proteins, transporters, and regulators. Protein-protein interactions revealed HP PCS8235_RS05845 made the highest interactions with other HPs and also has TRP structural motif along with virulent and pathogenic properties indicating it has critical cellular functions and might go under unconventional protein secretions. The second highest interacting protein HP PCS8235_RS02595 interacts with the Regulator of chromosomal segregation (RocS) which participates in chromosome segregation and nucleoid protection in <jats:italic>S<\/jats:italic>. <jats:italic>pneumoniae<\/jats:italic>. In this interacting network, 54% of protein members have virulent properties and 40% contain pathogenic properties. Among them, most of these proteins circulate in the cytoplasmic area and have hydrophilic properties. Finally, molecular docking and dynamics simulation demonstrated that the antimalarial drug Artenimol can act as a drug repurposing candidate against HP PCS8235_RS 04650 of <jats:italic>S<\/jats:italic>. <jats:italic>pneumoniae<\/jats:italic>. Hence, the present study could aid in drugs against <jats:italic>S<\/jats:italic>. <jats:italic>pneumoniae<\/jats:italic>.<\/jats:p>","DOI":"10.1371\/journal.pone.0272945","type":"journal-article","created":{"date-parts":[[2022,8,18]],"date-time":"2022-08-18T17:36:22Z","timestamp":1660844182000},"page":"e0272945","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Exploration of Streptococcus core genome to reveal druggable targets and novel therapeutics against S. pneumoniae"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6263-4960","authenticated-orcid":true,"given":"Zeshan Mahmud","family":"Chowdhury","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arittra","family":"Bhattacharjee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ishtiaque","family":"Ahammad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Uzzal","family":"Hossain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8964-7350","authenticated-orcid":true,"given":"Abdullah All","family":"Jaber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8279-9810","authenticated-orcid":true,"given":"Anisur","family":"Rahman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Preonath Chondrow","family":"Dev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9895-9233","authenticated-orcid":true,"given":"Md.","family":"Salimullah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chaman Ara","family":"Keya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2022,8,18]]},"reference":[{"key":"pone.0272945.ref001","doi-asserted-by":"crossref","first-page":"1302","DOI":"10.1007\/s12325-020-01248-7","article-title":"Burden of Community-Acquired Pneumonia and Unmet Clinical Needs","volume":"37","author":"J Ferreira-Coimbra","year":"2020","journal-title":"Adv Ther"},{"key":"pone.0272945.ref002","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1164\/rccm.201908-1581ST","article-title":"Diagnosis and Treatment of Adults with Community-acquired Pneumonia. An Official Clinical Practice Guideline of the American Thoracic Society and Infectious Diseases Society of America","volume":"200","author":"JP Metlay","year":"2019","journal-title":"Am J Respir Crit Care Med"},{"key":"pone.0272945.ref003","first-page":"010401","article-title":"Epidemiology and etiology of childhood pneumonia in 2010: estimates of incidence, severe morbidity, mortality, underlying risk factors and causative pathogens for 192 countries","volume":"3","author":"I Rudan","year":"2013","journal-title":"J Glob Health"},{"key":"pone.0272945.ref004","doi-asserted-by":"crossref","first-page":"1366","DOI":"10.3389\/fimmu.2018.01366","article-title":"Streptococcus pneumoniae\u2019s Virulence and Host Immunity: Aging, Diagnostics, and Prevention","volume":"9","author":"LRK Brooks","year":"2018","journal-title":"Front Immunol"},{"key":"pone.0272945.ref005","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1016\/S1473-3099(18)30310-4","article-title":"Estimates of the global, regional, and national morbidity, mortality, and aetiologies of lower respiratory infections in 195 countries, 1990\u20132016: a systematic analysis for the Global Burden of Disease Study 2016","volume":"18","author":"GBD 2016 Lower Respiratory Infections Collaborators","year":"2018","journal-title":"Lancet Infect Dis"},{"key":"pone.0272945.ref006","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1086\/591708","article-title":"Predominant Role of Bacterial Pneumonia as a Cause of Death in Pandemic Influenza: Implications for Pandemic Influenza Preparedness","volume":"198","author":"DM Morens","year":"2008","journal-title":"J Infect Dis"},{"key":"pone.0272945.ref007","doi-asserted-by":"crossref","first-page":"e64139","DOI":"10.7554\/eLife.64139","article-title":"Antimicrobial resistance and COVID-19: Intersections and implications","volume":"10","author":"GM Knight","year":"2021","journal-title":"eLife"},{"key":"pone.0272945.ref008","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1136\/thoraxjnl-2013-204282","article-title":"Risk factors for community-acquired pneumonia in adults in Europe: a literature review","volume":"68","author":"A Torres","year":"2013","journal-title":"Thorax"},{"key":"pone.0272945.ref009","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1016\/j.rmed.2012.04.008","article-title":"The natural history of community-acquired pneumonia in COPD patients: a population database analysis","volume":"106","author":"H M\u00fcllerova","year":"2012","journal-title":"Respir Med"},{"key":"pone.0272945.ref010","doi-asserted-by":"crossref","first-page":"12S","DOI":"10.1016\/S0002-9343(99)00103-5","article-title":"Unusual manifestations of invasive pneumococcal infection","volume":"107","author":"SN Taylor","year":"1999","journal-title":"Am J Med"},{"key":"pone.0272945.ref011","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1016\/S0140-6736(20)31550-6","article-title":"Complicated pneumonia in children","volume":"396","author":"FM de Benedictis","year":"2020","journal-title":"Lancet Lond Engl"},{"key":"pone.0272945.ref012","article-title":"Necrotizing pneumonia: a rare complication of pneumonia requiring special consideration","volume":"18","author":"T Yf","year":"2012","journal-title":"Curr Opin Pulm Med"},{"key":"pone.0272945.ref013","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/B978-1-4557-3383-5.00033-6","article-title":"Bacterial Pneumonia and Lung Abscess","author":"A Torres","year":"2016","journal-title":"Murray Nadels Textb Respir Med"},{"key":"pone.0272945.ref014","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1128\/CMR.00018-12","article-title":"Pediatric invasive pneumococcal disease in the United States in the era of pneumococcal conjugate vaccines","volume":"25","author":"TQ Tan","year":"2012","journal-title":"Clin Microbiol Rev"},{"key":"pone.0272945.ref015","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1586\/erv.10.145","article-title":"Global prevailing and emerging pediatric pneumococcal serotypes","volume":"10","author":"EDG McIntosh","year":"2011","journal-title":"Expert Rev Vaccines"},{"key":"pone.0272945.ref016","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1080\/21645515.2017.1409316","article-title":"Effectiveness and practical uses of 23-valent pneumococcal polysaccharide vaccine in healthy and special populations","volume":"14","author":"Y Wang","year":"2018","journal-title":"Hum Vaccines Immunother"},{"key":"pone.0272945.ref017","doi-asserted-by":"crossref","first-page":"212","DOI":"10.3390\/vaccines9030212","article-title":"Heightened Willingness toward Pneumococcal Vaccination in the Elderly Population in Shenzhen, China: A Cross-Sectional Study during the COVID-19 Pandemic","volume":"9","author":"M Zhang","year":"2021","journal-title":"Vaccines"},{"key":"pone.0272945.ref018","doi-asserted-by":"crossref","first-page":"3413","DOI":"10.1093\/jac\/dkaa350","article-title":"Implications of antibiotics use during the COVID-19 pandemic: present and future","volume":"75","author":"C Miranda","year":"2020","journal-title":"J Antimicrob Chemother"},{"key":"pone.0272945.ref019","doi-asserted-by":"crossref","first-page":"e361","DOI":"10.1097\/MJT.0000000000000551","article-title":"Antimicrobial Resistant Streptococcus pneumoniae: Prevalence, Mechanisms, and Clinical Implications","volume":"24","author":"R Cherazard","year":"2017","journal-title":"Am J Ther"},{"key":"pone.0272945.ref020","first-page":"1916","article-title":"Multi-drug resistance in Streptococcus pneumoniae among children in rural Vietnam more than doubled from 1999 to 2014","volume":"110","author":"M Larsson","year":"2021","journal-title":"Acta Paediatr Oslo Nor 1992"},{"key":"pone.0272945.ref021","first-page":"402","article-title":"Changes in antimicrobial resistance, serotypes and genotypes in Streptococcus pneumoniae over a 30-year period","volume":"16","author":"J Li\u00f1ares","year":"2010","journal-title":"Clin Microbiol Infect Off Publ Eur Soc Clin Microbiol Infect Dis"},{"key":"pone.0272945.ref022","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.csbj.2015.03.007","article-title":"Mechanisms and impact of genetic recombination in the evolution of Streptococcus pneumoniae","volume":"13","author":"C Chaguza","year":"2015","journal-title":"Comput Struct Biotechnol J"},{"key":"pone.0272945.ref023","first-page":"371","article-title":"Natural transformation and genome evolution in Streptococcus pneumoniae","volume":"33","author":"D Straume","year":"2015","journal-title":"Infect Genet Evol J Mol Epidemiol Evol Genet Infect Dis"},{"key":"pone.0272945.ref024","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2019.00094","article-title":"Competence in Streptococcus pneumoniae and Close Commensal Relatives: Mechanisms and Implications","volume":"9","author":"G Salvadori","year":"2019","journal-title":"Front Cell Infect Microbiol"},{"key":"pone.0272945.ref025","doi-asserted-by":"crossref","first-page":"R115","DOI":"10.1186\/gb-2011-12-11-r115","article-title":"Whole genome sequencing of penicillin-resistant Streptococcus pneumoniae reveals mutations in penicillin-binding proteins and in a putative iron permease","volume":"12","author":"F Fani","year":"2011","journal-title":"Genome Biol"},{"key":"pone.0272945.ref026","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2016.00098","article-title":"Macrolide Resistance in Streptococcus pneumoniae","volume":"6","author":"MR Schroeder","year":"2016","journal-title":"Front Cell Infect Microbiol"},{"issue":"Suppl 2","key":"pone.0272945.ref027","doi-asserted-by":"crossref","first-page":"S120","DOI":"10.1086\/428052","article-title":"Mechanisms of resistance to quinolones","volume":"41","author":"GA Jacoby","year":"2005","journal-title":"Clin Infect Dis Off Publ Infect Dis Soc Am"},{"key":"pone.0272945.ref028","first-page":"411","article-title":"Streptococcus pneumoniae: virulence factors and variation","volume":"16","author":"AM Mitchell","year":"2010","journal-title":"Clin Microbiol Infect Off Publ Eur Soc Clin Microbiol Infect Dis"},{"key":"pone.0272945.ref029","article-title":"In Silico Characterization of a Hypothetical Protein from Shigella dysenteriae ATCC 12039 Reveals a Pathogenesis-Related Protein of the Type-VI Secretion System","volume":"15","author":"MdF Rabbi","year":"2021","journal-title":"Bioinforma Biol Insights"},{"key":"pone.0272945.ref030","doi-asserted-by":"crossref","first-page":"e02734","DOI":"10.1016\/j.heliyon.2019.e02734","article-title":"In silico characterization of hypothetical proteins from Orientia tsutsugamushi str. Karp uncovers virulence genes","volume":"5","author":"N Imam","year":"2019","journal-title":"Heliyon"},{"key":"pone.0272945.ref031","doi-asserted-by":"crossref","first-page":"e7245","DOI":"10.1371\/journal.pone.0007245","article-title":"Bioinformatics and Structural Characterization of a Hypothetical Protein from Streptococcus mutans: Implication of Antibiotic Resistance","volume":"4","author":"J Nan","year":"2009","journal-title":"PLOS ONE"},{"key":"pone.0272945.ref032","doi-asserted-by":"crossref","first-page":"1740","DOI":"10.1093\/bioinformatics\/btw041","article-title":"BioCircos.js: an interactive Circos JavaScript library for biological data visualization on web applications","volume":"32","author":"Y Cui","year":"2016","journal-title":"Bioinforma Oxf Engl"},{"key":"pone.0272945.ref033","doi-asserted-by":"crossref","first-page":"W185","DOI":"10.1093\/nar\/gkab341","article-title":"EDGAR3.0: comparative genomics and phylogenomics on a scalable infrastructure","volume":"49","author":"MA Dieckmann","year":"2021","journal-title":"Nucleic Acids Res"},{"key":"pone.0272945.ref034","doi-asserted-by":"crossref","first-page":"1794","DOI":"10.1038\/s41598-018-20211-9","article-title":"GO FEAT: a rapid web-based functional annotation tool for genomic and transcriptomic data","volume":"8","author":"FA Araujo","year":"2018","journal-title":"Sci Rep"},{"key":"pone.0272945.ref035","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1385\/1-59259-890-0:571","volume-title":"The Proteomics Protocols Handbook","author":"E Gasteiger","year":"2005"},{"key":"pone.0272945.ref036","doi-asserted-by":"crossref","first-page":"1402","DOI":"10.1110\/ps.03479604","article-title":"Predicting subcellular localization of proteins for Gram-negative bacteria by support vector machines based on n-peptide compositions","volume":"13","author":"C-S Yu","year":"2004","journal-title":"Protein Sci Publ Protein Soc"},{"key":"pone.0272945.ref037","unstructured":"PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes\u2014PMC. [cited 10 May 2022]. https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC2887053\/"},{"key":"pone.0272945.ref038","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.ygeno.2013.04.011","article-title":"Putative drug and vaccine target protein identification using comparative genomic analysis of KEGG annotated metabolic pathways of Mycoplasma hyopneumoniae","volume":"102","author":"D Damte","year":"2013","journal-title":"Genomics"},{"key":"pone.0272945.ref039","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/1471-2180-5-58","article-title":"Non-classical protein secretion in bacteria","volume":"5","author":"JD Bendtsen","year":"2005","journal-title":"BMC Microbiol"},{"key":"pone.0272945.ref040","doi-asserted-by":"crossref","first-page":"19448","DOI":"10.1038\/s41598-019-55351-z","article-title":"OutCyte: a novel tool for predicting unconventional protein secretion","volume":"9","author":"L Zhao","year":"2019","journal-title":"Sci Rep"},{"key":"pone.0272945.ref041","doi-asserted-by":"crossref","first-page":"D271","DOI":"10.1093\/nar\/gkh024","article-title":"DEG: a database of essential genes","volume":"32","author":"R Zhang","year":"2004","journal-title":"Nucleic Acids Res"},{"key":"pone.0272945.ref042","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1186\/1471-2105-9-62","article-title":"VirulentPred: a SVM based prediction method for virulent proteins in bacterial pathogens","volume":"9","author":"A Garg","year":"2008","journal-title":"BMC Bioinformatics"},{"key":"pone.0272945.ref043","doi-asserted-by":"crossref","first-page":"e93907","DOI":"10.1371\/journal.pone.0093907","article-title":"MP3: A Software Tool for the Prediction of Pathogenic Proteins in Genomic and Metagenomic Data","volume":"9","author":"A Gupta","year":"2014","journal-title":"PLoS ONE"},{"key":"pone.0272945.ref044","doi-asserted-by":"crossref","first-page":"D561","DOI":"10.1093\/nar\/gkq973","article-title":"The STRING database in 2011: functional interaction networks of proteins, globally integrated and scored","volume":"39","author":"D Szklarczyk","year":"2011","journal-title":"Nucleic Acids Res"},{"key":"pone.0272945.ref045","doi-asserted-by":"crossref","first-page":"D901","DOI":"10.1093\/nar\/gkm958","article-title":"DrugBank: a knowledgebase for drugs, drug actions and drug targets","volume":"36","author":"DS Wishart","year":"2008","journal-title":"Nucleic Acids Res"},{"key":"pone.0272945.ref046","doi-asserted-by":"crossref","first-page":"W526","DOI":"10.1093\/nar\/gkh468","article-title":"Protein structure prediction and analysis using the Robetta server","volume":"32","author":"DE Kim","year":"2004","journal-title":"Nucleic Acids Res"},{"key":"pone.0272945.ref047","doi-asserted-by":"crossref","first-page":"3381","DOI":"10.1093\/nar\/gkg520","article-title":"SWISS-MODEL: An automated protein homology-modeling server","volume":"31","author":"T Schwede","year":"2003","journal-title":"Nucleic Acids Res"},{"key":"pone.0272945.ref048","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1515\/bmc.2010.022","article-title":"A fresh look at the Ramachandran plot and the occurrence of standard structures in proteins","volume":"1","author":"SA Hollingsworth","year":"2010","journal-title":"Biomol Concepts"},{"key":"pone.0272945.ref049","unstructured":"Small-Molecule Library Screening by Docking with PyRx | Springer Nature Experiments. [cited 10 May 2022]. https:\/\/experiments.springernature.com\/articles\/10.1007\/978-1-4939-2269-7_19"},{"key":"pone.0272945.ref050","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.1093\/bioinformatics\/btv011","article-title":"Using isoelectric point to determine the pH for initial protein crystallization trials","volume":"31","author":"J Kirkwood","year":"2015","journal-title":"Bioinformatics"},{"key":"pone.0272945.ref051","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/0003-2697(89)90602-7","article-title":"Calculation of protein extinction coefficients from amino acid sequence data","volume":"182","author":"SC Gill","year":"1989","journal-title":"Anal Biochem"},{"key":"pone.0272945.ref052","doi-asserted-by":"crossref","first-page":"339","DOI":"10.2174\/0929866526666190228144219","article-title":"Applicability of Instability Index for In vitro Protein Stability Prediction","volume":"26","author":"DG Gamage","year":"2019","journal-title":"Protein Pept Lett"},{"key":"pone.0272945.ref053","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1016\/j.jgeb.2018.05.003","article-title":"In silico studies on bacterial xylanase enzyme: Structural and functional insight","volume":"16","author":"B Dutta","year":"2018","journal-title":"J Genet Eng Biotechnol"},{"key":"pone.0272945.ref054","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1128\/MMBR.00016-10","article-title":"Origins and Evolution of Antibiotic Resistance","volume":"74","author":"J Davies","year":"2010","journal-title":"Microbiol Mol Biol Rev MMBR"},{"key":"pone.0272945.ref055","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s12088-016-0625-1","article-title":"Bacterial Virulence Factors: Secreted for Survival","volume":"57","author":"AK Sharma","year":"2017","journal-title":"Indian J Microbiol"},{"key":"pone.0272945.ref056","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1038\/nrd2897","article-title":"Subcellular targeting strategies for drug design and delivery","volume":"9","author":"L Rajendran","year":"2010","journal-title":"Nat Rev Drug Discov"},{"key":"pone.0272945.ref057","doi-asserted-by":"crossref","first-page":"e0262956","DOI":"10.1371\/journal.pone.0262956","article-title":"Bacterial profile, antimicrobial susceptibility patterns, and associated factors of community-acquired pneumonia among adult patients in Gondar, Northwest Ethiopia: A cross-sectional study","volume":"17","author":"M Assefa","year":"2022","journal-title":"PLOS ONE"},{"key":"pone.0272945.ref058","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s42003-020-01290-9","article-title":"Bacterial genome-wide association study of hyper-virulent pneumococcal serotype 1 identifies genetic variation associated with neurotropism","volume":"3","author":"C Chaguza","year":"2020","journal-title":"Commun Biol"},{"key":"pone.0272945.ref059","first-page":"334","article-title":"Mechanisms of genome evolution of Streptococcus","volume":"33","author":"CP Andam","year":"2015","journal-title":"Infect Genet Evol J Mol Epidemiol Evol Genet Infect Dis"},{"key":"pone.0272945.ref060","doi-asserted-by":"crossref","first-page":"8186","DOI":"10.1128\/JB.00690-07","article-title":"Comparative genomic analyses of seventeen Streptococcus pneumoniae strains: Insights into the pneumococcal supragenome","volume":"189","author":"NL Hiller","year":"2007","journal-title":"J Bacteriol"},{"key":"pone.0272945.ref061","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1007\/s10096-013-1993-7","article-title":"The virulence of Streptococcus mutans and the ability to form biofilms","volume":"33","author":"W Krzy\u015bciak","year":"2014","journal-title":"Eur J Clin Microbiol Infect Dis Off Publ Eur Soc Clin Microbiol"},{"key":"pone.0272945.ref062","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1006\/jmbi.1994.1711","article-title":"Computer analysis of bacterial haloacid dehalogenases defines a large superfamily of hydrolases with diverse specificity. Application of an iterative approach to database search","volume":"244","author":"EV Koonin","year":"1994","journal-title":"J Mol Biol"},{"key":"pone.0272945.ref063","doi-asserted-by":"crossref","first-page":"18678","DOI":"10.1074\/jbc.M115.657916","article-title":"Functional Diversity of Haloacid Dehalogenase Superfamily Phosphatases from Saccharomyces cerevisiae: BIOCHEMICAL, STRUCTURAL, AND EVOLUTIONARY INSIGHTS","volume":"290","author":"E Kuznetsova","year":"2015","journal-title":"J Biol Chem"},{"key":"pone.0272945.ref064","doi-asserted-by":"crossref","first-page":"5729","DOI":"10.1128\/JB.00425-08","article-title":"RadC, a Misleading Name?","volume":"190","author":"L Attaiech","year":"2008","journal-title":"J Bacteriol"},{"key":"pone.0272945.ref065","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1111\/mmi.12186","article-title":"A complex of YlbF, YmcA and YaaT regulates sporulation, competence and biofilm formation by accelerating the phosphorylation of Spo0A","volume":"88","author":"VJ Carabetta","year":"2013","journal-title":"Mol Microbiol"},{"key":"pone.0272945.ref066","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2017.02331","article-title":"A Comprehensive Overview of Online Resources to Identify and Predict Bacterial Essential Genes","volume":"8","author":"C Peng","year":"2017","journal-title":"Front Microbiol"},{"key":"pone.0272945.ref067","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1128\/IAI.01035-12","article-title":"Tetratricopeptide Repeat Motifs in the World of Bacterial Pathogens: Role in Virulence Mechanisms","volume":"81","author":"L Cerveny","year":"2013","journal-title":"Infect Immun"},{"key":"pone.0272945.ref068","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1111\/mmi.12331","article-title":"In vivo organization of the FtsZ-ring by ZapA and ZapB revealed by quantitative super-resolution microscopy","volume":"89","author":"J Buss","year":"2013","journal-title":"Mol Microbiol"},{"key":"pone.0272945.ref069","doi-asserted-by":"crossref","first-page":"2544","DOI":"10.1101\/gad.1014102","article-title":"A widely conserved bacterial cell division protein that promotes assembly of the tubulin-like protein FtsZ","volume":"16","author":"FJ Gueiros-Filho","year":"2002","journal-title":"Genes Dev"},{"key":"pone.0272945.ref070","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.1038\/s41564-019-0472-z","article-title":"RocS drives chromosome segregation and nucleoid protection in Streptococcus pneumoniae","volume":"4","author":"C Mercy","year":"2019","journal-title":"Nat Microbiol"},{"key":"pone.0272945.ref071","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.micpath.2019.03.024","article-title":"Computational screening of potential drug targets for pathogens causing bacterial pneumonia","volume":"130","author":"S Nayak","year":"2019","journal-title":"Microb Pathog"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0272945","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,18]],"date-time":"2022-08-18T17:37:20Z","timestamp":1660844240000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0272945"}},"subtitle":[],"editor":[{"given":"Jamunarani","family":"Vadivelu","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2022,8,18]]},"references-count":71,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,8,18]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0272945","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,18]]}}}