{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T17:48:55Z","timestamp":1772214535888,"version":"3.50.1"},"reference-count":77,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T00:00:00Z","timestamp":1661731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Global Good Fund I, LLC"},{"name":"UKRI Connecting Capabilities Fund","award":["BSA14"],"award-info":[{"award-number":["BSA14"]}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["218676\/Z\/19\/Z"],"award-info":[{"award-number":["218676\/Z\/19\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Global Good Fund I, LLC"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The evaluation of protein antigens as putative serologic biomarkers of infection has increasingly shifted to high-throughput, multiplex approaches such as the protein microarray. In vitro transcription\/translation (IVTT) systems\u2013a similarly high-throughput protein expression method\u2013are already widely utilised in the production of protein microarrays, though purified recombinant proteins derived from more traditional whole cell based expression systems also play an important role in biomarker characterisation. Here we have performed a side-by-side comparison of antigen-matched protein targets from an IVTT and purified recombinant system, on the same protein microarray. The magnitude and range of antibody responses to purified recombinants was found to be greater than that of IVTT proteins, and responses between targets from different expression systems did not clearly correlate. However, responses between amino acid sequence-matched targets from each expression system were more closely correlated. Despite the lack of a clear correlation between antigen-matched targets produced in each expression system, our data indicate that protein microarrays produced using either method can be used confidently, in a context dependent manner, though care should be taken when comparing data derived from contrasting approaches.<\/jats:p>","DOI":"10.1371\/journal.pone.0273106","type":"journal-article","created":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T13:34:08Z","timestamp":1661780048000},"page":"e0273106","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Plasmodium falciparum serology: A comparison of two protein production methods for analysis of antibody responses by protein microarray"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3027-7380","authenticated-orcid":true,"given":"Tate","family":"Oulton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9741-512X","authenticated-orcid":true,"given":"Joshua","family":"Obiero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel","family":"Rodriguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isaac","family":"Ssewanyana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca A.","family":"Dabbs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8143-2782","authenticated-orcid":true,"given":"Christine M.","family":"Bachman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryan","family":"Greenhouse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4863-075X","authenticated-orcid":true,"given":"Chris","family":"Drakeley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phil L.","family":"Felgner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6647-0166","authenticated-orcid":true,"given":"Will","family":"Stone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2733-0037","authenticated-orcid":true,"given":"Kevin K. A.","family":"Tetteh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2022,8,29]]},"reference":[{"key":"pone.0273106.ref001","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/j.pt.2007.08.023","article-title":"Serology: a robust indicator of malaria transmission intensity?","volume":"23","author":"P Corran","year":"2007","journal-title":"Trends Parasitol"},{"key":"pone.0273106.ref002","doi-asserted-by":"crossref","first-page":"5108","DOI":"10.1073\/pnas.0408725102","article-title":"Estimating medium- and long-term trends in malaria transmission by using serological markers of malaria exposure","volume":"102","author":"CJ Drakeley","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"key":"pone.0273106.ref003","doi-asserted-by":"crossref","first-page":"e6083","DOI":"10.1371\/journal.pone.0006083","article-title":"Rapid assessment of malaria transmission using age-specific sero-conversion rates.","volume":"4","author":"L Stewart","year":"2009","journal-title":"PloS One"},{"key":"pone.0273106.ref004","doi-asserted-by":"crossref","first-page":"392","DOI":"10.3201\/eid1603.090732","article-title":"Serologic markers for detecting malaria in areas of low endemicity, Somalia, 2008","volume":"16","author":"T Bousema","year":"2010","journal-title":"Emerg Infect Dis"},{"key":"pone.0273106.ref005","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1128\/cdli.2.1.30-34.1995","article-title":"Antigenicity and immunogenicity of recombinant glutamate-rich protein of Plasmodium falciparum expressed in Escherichia coli","volume":"2","author":"M Theisen","year":"1995","journal-title":"Clin Diagn Lab Immunol"},{"key":"pone.0273106.ref006","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1046\/j.1365-3024.1997.d01-182.x","article-title":"Cross-reactive cellular immune response to circumsporozoite proteins of Plasmodium vivax and P. falciparum in malaria-exposed individuals","volume":"19","author":"LH Carvalho","year":"1997","journal-title":"Parasite Immunol"},{"key":"pone.0273106.ref007","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1006\/expr.1998.4315","article-title":"Plasmodium falciparum: heterologous synthesis of the transmission-blocking vaccine candidate Pfs48\/45 in recombinant vaccinia virus-infected cells","volume":"90","author":"RL Milek","year":"1998","journal-title":"Exp Parasitol"},{"key":"pone.0273106.ref008","first-page":"973","article-title":"Differential recognition of Plasmodium falciparum merozoite surface protein 2 variants by antibodies from malaria patients in Brazil","volume":"10","author":"KA Kanunfre","year":"2003","journal-title":"Clin Diagn Lab Immunol"},{"key":"pone.0273106.ref009","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1038\/nature01097","article-title":"Genome sequence of the human malaria parasite Plasmodium falciparum","volume":"419","author":"MJ Gardner","year":"2002","journal-title":"Nature"},{"key":"pone.0273106.ref010","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1038\/nature01107","article-title":"A proteomic view of the Plasmodium falciparum life cycle","volume":"419","author":"L Florens","year":"2002","journal-title":"Nature"},{"key":"pone.0273106.ref011","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1586\/erv.10.50","article-title":"Antibody-profiling technologies for studying humoral responses to infectious agents.","volume":"9","author":"PD Burbelo","year":"2010","journal-title":"Expert Rev Vaccines"},{"key":"pone.0273106.ref012","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/j.jprot.2013.10.010","article-title":"Protein arrays as tool for studies at the host-pathogen interface","volume":"94","author":"R Manzano-Rom\u00e1n","year":"2013","journal-title":"J Proteomics"},{"key":"pone.0273106.ref013","first-page":"88","article-title":"Microscopic and Submicroscopic Asymptomatic Plasmodium falciparum Infections in Ghanaian Children and Protection against Febrile Malaria","author":"B Adu","year":"2020","journal-title":"Infect Immun"},{"key":"pone.0273106.ref014","article-title":"Antibody Profiles to P. falciparum Antigens Over Time Characterize Acute and Long-Term Malaria Exposure in an Area of Low and Unstable Transmission","author":"BN Ondigo","year":"2020","journal-title":"Am J Trop Med Hyg"},{"key":"pone.0273106.ref015","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1073\/pnas.0408782102","article-title":"Profiling the humoral immune response to infection by using proteome microarrays: High-throughput vaccine and diagnostic antigen discovery","volume":"102","author":"DH Davies","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"key":"pone.0273106.ref016","doi-asserted-by":"crossref","first-page":"e0004563","DOI":"10.1371\/journal.pntd.0004563","article-title":"Antibody Profiling in Naive and Semi-immune Individuals Experimentally Challenged with Plasmodium vivax Sporozoites.","volume":"10","author":"M Arevalo-Herrera","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"key":"pone.0273106.ref017","doi-asserted-by":"crossref","first-page":"6958","DOI":"10.1073\/pnas.1001323107","article-title":"A prospective analysis of the Ab response to Plasmodium falciparum before and after a malaria season by protein microarray","volume":"107","author":"PD Crompton","year":"2010","journal-title":"Proc Natl Acad Sci U S A"},{"key":"pone.0273106.ref018","doi-asserted-by":"crossref","first-page":"I508","DOI":"10.1093\/bioinformatics\/btm207","article-title":"From protein microarrays to diagnostic antigen discovery: a study of the pathogen Francisella tularensis","volume":"23","author":"S Sundaresh","year":"2007","journal-title":"Bioinformatics"},{"key":"pone.0273106.ref019","first-page":"E4438","article-title":"Novel serologic biomarkers provide accurate estimates of recent Plasmodium falciparum exposure for individuals and communities","volume":"112","author":"DA Helb","year":"2015","journal-title":"Proc Natl Acad Sci"},{"key":"pone.0273106.ref020","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1038\/s41467-017-02646-2","article-title":"Unravelling the immune signature of Plasmodium falciparum transmission-reducing immunity","volume":"9","author":"WJR Stone","year":"2018","journal-title":"Nat Commun"},{"key":"pone.0273106.ref021","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2018.03300","article-title":"Antibody Responses to Antigenic Targets of Recent Exposure Are Associated With Low-Density Parasitemia in Controlled Human Plasmodium falciparum Infections.","volume":"9","author":"LL van den Hoogen","year":"2019","journal-title":"Front Microbiol"},{"key":"pone.0273106.ref022","article-title":"Antibody Response to a Nucleocapsid Epitope as a Marker for COVID-19 Disease Severity.","author":"S Sen","year":"2020","journal-title":"BioRxiv Prepr Serv Biol"},{"key":"pone.0273106.ref023","article-title":"Analysis of Serologic Cross-Reactivity Between Common Human Coronaviruses and SARS-CoV-2 Using Coronavirus Antigen Microarray.","author":"S Khan","year":"2020","journal-title":"BioRxiv Prepr Serv Biol."},{"key":"pone.0273106.ref024","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1038\/s41541-019-0131-7","article-title":"Interrogation of a live-attenuated enterotoxigenic Escherichia coli vaccine highlights features unique to wild-type infection","volume":"4","author":"S Chakraborty","year":"2019","journal-title":"NPJ Vaccines"},{"key":"pone.0273106.ref025","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.cbpa.2013.02.014","article-title":"Cell-free translation of peptides and proteins: from high throughput screening to clinical production","volume":"17","author":"CJ Murray","year":"2013","journal-title":"Curr Opin Chem Biol"},{"key":"pone.0273106.ref026","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.synbio.2019.10.003","article-title":"Methods to reduce variability in E. Coli-based cell-free protein expression experiments.","volume":"4","author":"JL Dopp","year":"2019","journal-title":"Synth Syst Biotechnol"},{"key":"pone.0273106.ref027","doi-asserted-by":"crossref","first-page":"102703","DOI":"10.1016\/j.copbio.2022.102703","article-title":"Systems biology-based analysis of cell-free systems","volume":"75","author":"H Sridharan","year":"2022","journal-title":"Curr Opin Biotechnol"},{"key":"pone.0273106.ref028","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1038\/s41576-019-0186-3","article-title":"Cell-free gene expression: an expanded repertoire of applications","volume":"21","author":"AD Silverman","year":"2020","journal-title":"Nat Rev Genet"},{"key":"pone.0273106.ref029","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/978-1-0716-1562-1_10","article-title":"Protein Arrays for the Identification of Seroreactive Protein Markers for Infectious Diseases.","volume":"2344","author":"A Venkatesh","year":"2021","journal-title":"Methods Mol Biol Clifton NJ"},{"key":"pone.0273106.ref030","doi-asserted-by":"crossref","first-page":"e00061","DOI":"10.1128\/mSphereDirect.00061-19","article-title":"Distinct Antibody Signatures Associated with Different Malaria Transmission Intensities in Zambia and Zimbabwe.","volume":"4","author":"T Kobayashi","year":"2019","journal-title":"mSphere"},{"key":"pone.0273106.ref031","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1038\/s41591-020-0841-4","article-title":"Development and validation of serological markers for detecting recent Plasmodium vivax infection","volume":"26","author":"RJ Longley","year":"2020","journal-title":"Nat Med"},{"key":"pone.0273106.ref032","doi-asserted-by":"crossref","first-page":"2669","DOI":"10.3389\/fimmu.2019.02669","article-title":"The N-Terminal Region of Plasmodium falciparum MSP10 Is a Target of Protective Antibodies in Malaria and Is Important for PfGAMA\/PfMSP10 Interaction.","volume":"10","author":"H Nagaoka","year":"2019","journal-title":"Front Immunol"},{"key":"pone.0273106.ref033","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1038\/s41467-019-08528-z","article-title":"Targets of complement-fixing antibodies in protective immunity against malaria in children","volume":"10","author":"L Reiling","year":"2019","journal-title":"Nat Commun"},{"key":"pone.0273106.ref034","doi-asserted-by":"crossref","first-page":"605367","DOI":"10.3389\/fcimb.2020.605367","article-title":"Identification of a Novel RAMA\/RON3 Rhoptry Protein Complex in Plasmodium falciparum Merozoites","volume":"10","author":"D Ito","year":"2020","journal-title":"Front Cell Infect Microbiol"},{"key":"pone.0273106.ref035","doi-asserted-by":"crossref","first-page":"2866","DOI":"10.3389\/fimmu.2018.02866","article-title":"KILchip v1.0: A Novel Plasmodium falciparum Merozoite Protein Microarray to Facilitate Malaria Vaccine Candidate Prioritization","volume":"9","author":"G Kamuyu","year":"2018","journal-title":"Front Immunol"},{"key":"pone.0273106.ref036","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/gkm047","article-title":"A versatile ligation-independent cloning method suitable for high-throughput expression screening applications","volume":"35","author":"NS Berrow","year":"2007","journal-title":"Nucleic Acids Res"},{"key":"pone.0273106.ref037","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1107\/S0907444906029775","article-title":"Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE","volume":"62","author":"PM Alzari","year":"2006","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"pone.0273106.ref038","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/978-1-4939-9624-7_2","article-title":"Overview of a High-Throughput Pipeline for Streamlining the Production of Recombinant Proteins.","volume":"2025","author":"JE Nettleship","year":"2019","journal-title":"Methods Mol Biol Clifton NJ"},{"key":"pone.0273106.ref039","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1038\/nbt1029","article-title":"Recombinant protein folding and misfolding in Escherichia coli","volume":"22","author":"F Baneyx","year":"2004","journal-title":"Nat Biotechnol"},{"key":"pone.0273106.ref040","article-title":"Optimization of Methods for the Production and Refolding of Biologically Active Disulfide Bond-Rich Antibody Fragments in Microbial Hosts.","volume":"9","author":"B Ban","year":"2020","journal-title":"Antibodies Basel Switz."},{"key":"pone.0273106.ref041","doi-asserted-by":"crossref","DOI":"10.1002\/0471140864.ps0524s61","article-title":"Strategies to Optimize Protein Expression in E. coli.","volume":"61","author":"DM Francis","year":"2010","journal-title":"Curr Protoc Protein Sci"},{"key":"pone.0273106.ref042","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.molbiopara.2006.10.011","article-title":"Genome-scale protein expression and structural biology of Plasmodium falciparum and related Apicomplexan organisms","volume":"151","author":"M Vedadi","year":"2007","journal-title":"Mol Biochem Parasitol"},{"key":"pone.0273106.ref043","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.molbiopara.2006.03.011","article-title":"Heterologous expression of proteins from Plasmodium falciparum: results from 1000 genes","volume":"148","author":"C Mehlin","year":"2006","journal-title":"Mol Biochem Parasitol"},{"key":"pone.0273106.ref044","doi-asserted-by":"crossref","first-page":"903","DOI":"10.4269\/ajtmh.14-0312","article-title":"Malaria transmission, infection, and disease at three sites with varied transmission intensity in Uganda: implications for malaria control","volume":"92","author":"MR Kamya","year":"2015","journal-title":"Am J Trop Med Hyg"},{"key":"pone.0273106.ref045","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/0166-6851(94)90144-9","article-title":"Expression of the 19-kilodalton carboxy-terminal fragment of the Plasmodium falciparum merozoite surface protein-1 in Escherichia coli as a correctly folded protein","volume":"64","author":"PA Burghaus","year":"1994","journal-title":"Mol Biochem Parasitol"},{"key":"pone.0273106.ref046","doi-asserted-by":"crossref","first-page":"1833","DOI":"10.1128\/IAI.71.4.1833-1842.2003","article-title":"Repeat sequences in block 2 of Plasmodium falciparum merozoite surface protein 1 are targets of antibodies associated with protection from malaria","volume":"71","author":"SD Polley","year":"2003","journal-title":"Infect Immun"},{"key":"pone.0273106.ref047","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1091\/mbc.e02-04-0240","article-title":"etramps, a new Plasmodium falciparum gene family coding for developmentally regulated and highly charged membrane proteins located at the parasite-host cell interface","volume":"14","author":"T Spielmann","year":"2003","journal-title":"Mol Biol Cell"},{"key":"pone.0273106.ref048","doi-asserted-by":"crossref","first-page":"e50","DOI":"10.1086\/656413","article-title":"Association between naturally acquired antibodies to erythrocyte-binding antigens of Plasmodium falciparum and protection from malaria and high-density parasitemia","volume":"51","author":"JS Richards","year":"2010","journal-title":"Clin Infect Dis Off Publ Infect Dis Soc Am"},{"key":"pone.0273106.ref049","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1016\/S0264-410X(00)00245-0","article-title":"Differences in epitope recognition, isotype and titer of antisera to Plasmodium falciparum merozoite surface protein 4 raised by different modes of DNA or protein immunization","volume":"19","author":"L Wang","year":"2000","journal-title":"Vaccine"},{"key":"pone.0273106.ref050","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S0166-6851(01)00458-3","article-title":"The Plasmodium vivax homologues of merozoite surface proteins 4 and 5 from Plasmodium falciparum are expressed at different locations in the merozoite","volume":"120","author":"CG Black","year":"2002","journal-title":"Mol Biochem Parasitol"},{"key":"pone.0273106.ref051","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0166-6851(01)00336-X","article-title":"The 22 kDa component of the protein complex on the surface of Plasmodium falciparum merozoites is derived from a larger precursor, merozoite surface protein 7","volume":"117","author":"JA Pachebat","year":"2001","journal-title":"Mol Biochem Parasitol"},{"key":"pone.0273106.ref052","doi-asserted-by":"crossref","first-page":"1869","DOI":"10.1128\/EC.00218-09","article-title":"Novel putative glycosylphosphatidylinositol-anchored micronemal antigen of Plasmodium falciparum that binds to erythrocytes","volume":"8","author":"L Hinds","year":"2009","journal-title":"Eukaryot Cell"},{"key":"pone.0273106.ref053","doi-asserted-by":"crossref","first-page":"7431","DOI":"10.1074\/jbc.M610562200","article-title":"Fine mapping of an epitope recognized by an invasion-inhibitory monoclonal antibody on the malaria vaccine candidate apical membrane antigen 1","volume":"282","author":"CR Collins","year":"2007","journal-title":"J Biol Chem"},{"key":"pone.0273106.ref054","doi-asserted-by":"crossref","first-page":"e47","DOI":"10.1093\/nar\/gkv007","article-title":"limma powers differential expression analyses for RNA-sequencing and microarray studies","volume":"43","author":"ME Ritchie","year":"2015","journal-title":"Nucleic Acids Res"},{"key":"pone.0273106.ref055","doi-asserted-by":"crossref","first-page":"e1000273","DOI":"10.1371\/journal.ppat.1000273","article-title":"An inhibitory antibody blocks interactions between components of the malarial invasion machinery.","volume":"5","author":"CR Collins","year":"2009","journal-title":"PLoS Pathog"},{"key":"pone.0273106.ref056","doi-asserted-by":"crossref","first-page":"795","DOI":"10.4049\/jimmunol.1300778","article-title":"Identification and Prioritization of Merozoite Antigens as Targets of Protective Human Immunity to Plasmodium falciparum Malaria for Vaccine and Biomarker Development","volume":"191","author":"JS Richards","year":"2013","journal-title":"J Immunol"},{"key":"pone.0273106.ref057","doi-asserted-by":"crossref","first-page":"3952","DOI":"10.1038\/s41598-020-60551-z","article-title":"Microarray analyses reveal strain-specific antibody responses to Plasmodium falciparum apical membrane antigen 1 variants following natural infection and vaccination.","volume":"10","author":"JA Bailey","year":"2020","journal-title":"Sci Rep"},{"key":"pone.0273106.ref058","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/j.chom.2019.10.012","article-title":"Protective Immunity against Severe Malaria in Children Is Associated with a Limited Repertoire of Antibodies to Conserved PfEMP1 Variants.","volume":"26","author":"SK Tessema","year":"2019","journal-title":"Cell Host Microbe"},{"key":"pone.0273106.ref059","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1074\/mcp.RA118.001256","article-title":"Immune Signature Against Plasmodium falciparum Antigens Predicts Clinical Immunity in Distinct Malaria Endemic Communities","volume":"19","author":"C Proietti","year":"2020","journal-title":"Mol Cell Proteomics MCP"},{"key":"pone.0273106.ref060","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1186\/s12936-019-2905-9","article-title":"Serologic responses to the PfEMP1 DBL-CIDR head structure may be a better indicator of malaria exposure than those to the DBL-\u03b1 tag","volume":"18","author":"EM Stucke","year":"2019","journal-title":"Malar J"},{"key":"pone.0273106.ref061","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1186\/s12936-019-2771-5","article-title":"Hospital-derived antibody profiles of malaria patients in Southwest India.","volume":"18","author":"A Venkatesh","year":"2019","journal-title":"Malar J"},{"key":"pone.0273106.ref062","article-title":"Epitope-specific antibody responses to a Plasmodium falciparum subunit vaccine target in a malaria-endemic population","author":"DJ Friedman-Klabanoff","year":"2020","journal-title":"J Infect Dis"},{"key":"pone.0273106.ref063","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1186\/s12916-020-01785-6","article-title":"Sero-epidemiological evaluation of malaria transmission in The Gambia before and after mass drug administration.","volume":"18","author":"L Wu","year":"2020","journal-title":"BMC Med"},{"key":"pone.0273106.ref064","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1186\/s12936-020-03434-y","article-title":"Malaria serology data from the Guiana shield: first insight in IgG antibody responses to Plasmodium falciparum, Plasmodium vivax and Plasmodium malariae antigens in Suriname.","volume":"19","author":"MY Labadie-Bracho","year":"2020","journal-title":"Malar J"},{"key":"pone.0273106.ref065","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1186\/s12916-020-01724-5","article-title":"Antibody responses to a suite of novel serological markers for malaria surveillance demonstrate strong correlation with clinical and parasitological infection across seasons and transmission settings in The Gambia.","volume":"18","author":"L Wu","year":"2020","journal-title":"BMC Med"},{"key":"pone.0273106.ref066","article-title":"School-based sero-surveys to assess the validity of using routine health facility data to target malaria interventions in the Central Highlands of Madagascar","author":"L Steinhardt","year":"2020","journal-title":"J Infect Dis"},{"key":"pone.0273106.ref067","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1186\/s12936-020-03199-4","article-title":"Screening for malaria antigen and anti-malarial IgG antibody in forcibly-displaced Myanmar nationals: Cox\u2019s Bazar district, Bangladesh, 2018.","volume":"19","author":"A Lu","year":"2020","journal-title":"Malar J"},{"key":"pone.0273106.ref068","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/s12916-019-1482-7","article-title":"Using health facility-based serological surveillance to predict receptive areas at risk of malaria outbreaks in elimination areas.","volume":"18","author":"H Surendra","year":"2020","journal-title":"BMC Med"},{"key":"pone.0273106.ref069","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/s12936-020-3111-5","article-title":"A panel of recombinant proteins from human-infective Plasmodium species for serological surveillance","volume":"19","author":"N M\u00fcller-Sienerth","year":"2020","journal-title":"Malar J"},{"key":"pone.0273106.ref070","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1186\/s12936-019-2874-z","article-title":"Multiplex serology demonstrate cumulative prevalence and spatial distribution of malaria in Ethiopia.","volume":"18","author":"A Assefa","year":"2019","journal-title":"Malar J"},{"key":"pone.0273106.ref071","doi-asserted-by":"crossref","first-page":"e135","DOI":"10.1093\/nar\/gkl462","article-title":"Troubleshooting coupled in vitro transcription\u2013translation system derived from Escherichia coli cells: synthesis of high-yield fully active proteins","volume":"34","author":"MB Iskakova","year":"2006","journal-title":"Nucleic Acids Res"},{"key":"pone.0273106.ref072","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.biochi.2013.11.025","article-title":"Synthesis of 2.3 mg\/ml of protein with an all Escherichia coli cell-free transcription\u2013translation system","volume":"99","author":"F Caschera","year":"2014","journal-title":"Biochimie"},{"key":"pone.0273106.ref073","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1021\/acssynbio.6b00250","article-title":"Cell-Free Translation Is More Variable than Transcription.","volume":"6","author":"F Chizzolini","year":"2017","journal-title":"ACS Synth Biol"},{"key":"pone.0273106.ref074","doi-asserted-by":"crossref","first-page":"46086","DOI":"10.1038\/srep46086","article-title":"Immunoscreening of Plasmodium falciparum proteins expressed in a wheat germ cell-free system reveals a novel malaria vaccine candidate","volume":"7","author":"M Morita","year":"2017","journal-title":"Sci Rep"},{"key":"pone.0273106.ref075","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1016\/j.vaccine.2017.01.001","article-title":"Antibody profiles to wheat germ cell-free system synthesized Plasmodium falciparum proteins correlate with protection from symptomatic malaria in Uganda","volume":"35","author":"BN Kanoi","year":"2017","journal-title":"Vaccine"},{"key":"pone.0273106.ref076","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.jsb.2010.02.006","article-title":"Recent advances in the production of proteins in insect and mammalian cells for structural biology","volume":"172","author":"JE Nettleship","year":"2010","journal-title":"J Struct Biol"},{"key":"pone.0273106.ref077","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.pep.2017.03.016","article-title":"Streamlining workflow and automation to accelerate laboratory scale protein production","volume":"133","author":"J Konczal","year":"2017","journal-title":"Protein Expr Purif"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0273106","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T13:35:38Z","timestamp":1661780138000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0273106"}},"subtitle":[],"editor":[{"given":"Takafumi","family":"Tsuboi","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2022,8,29]]},"references-count":77,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,8,29]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0273106","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.12.15.422854","asserted-by":"object"}]},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,29]]}}}