{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T18:19:57Z","timestamp":1793038797686,"version":"4.5.0"},"reference-count":52,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2022,8,25]],"date-time":"2022-08-25T00:00:00Z","timestamp":1661385600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    The sympathetic nervous system is highly involved in the regulation of gastrointestinal functions such as luminal alkalinisation and fluid absorption. However, the exact mechanisms are not clear. This study aimed to delineate how \u03b1\n                    <jats:sub>2<\/jats:sub>\n                    -adrenergic receptor stimulation reduces duodenal luminal alkalinisation and induces net fluid absorption. This was tested by perfusing the duodenum of anesthetized rats with isotonic solutions devoid of Cl\n                    <jats:sup>-<\/jats:sup>\n                    and\/or Na\n                    <jats:sup>+<\/jats:sup>\n                    , in the absence and presence of the \u03b1\n                    <jats:sub>2<\/jats:sub>\n                    -adrenoceptor agonist clonidine. The clonidine was also studied in rats treated with dimethylamiloride (a Na\n                    <jats:sup>+<\/jats:sup>\n                    \/H\n                    <jats:sup>+<\/jats:sup>\n                    exchange inhibitor), vasoactive intestinal peptide, and the nicotinic receptor antagonist hexamethonium. Clonidine reduced luminal alkalinisation and induced net fluid absorption. The Cl\n                    <jats:sup>-<\/jats:sup>\n                    -free solution decreased luminal alkalinisation and abolished net fluid absorption, but did not prevent clonidine from doing so. Both the Na\n                    <jats:sup>+<\/jats:sup>\n                    -free solution and luminal dimethylamiloride increased luminal alkalinisation and abolished net fluid absorption, effects counteracted by clonidine. The NaCl-free solution (D-mannitol) did not affect luminal alkalinisation, but reduced net fluid absorption. Clonidine reduced luminal alkalinisation and induced net fluid absorption in rats perfused luminally with mannitol. However, clonidine did not affect the vasoactive intestinal peptide-induced increase in luminal alkalinisation or fluid secretion. Pre-treatment with hexamethonium abolished the effects of clonidine on luminal alkalinisation and net fluid flux. In summary, our\n                    <jats:italic>in vivo<\/jats:italic>\n                    experiments showed that clonidine-induced reduction in luminal alkalinisation and induction of net fluid absorption was unrelated to luminal Na\n                    <jats:sup>+<\/jats:sup>\n                    and Cl\n                    <jats:sup>-<\/jats:sup>\n                    , or to apical Na\n                    <jats:sup>+<\/jats:sup>\n                    \/H\n                    <jats:sup>+<\/jats:sup>\n                    or Cl\n                    <jats:sup>-<\/jats:sup>\n                    \/HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>-<\/jats:sup>\n                    exchangers. Instead, clonidine seems to exert its effects via suppression of nicotinic receptor-activated acetylcholine secretomotor neurons.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0273208","type":"journal-article","created":{"date-parts":[[2022,8,25]],"date-time":"2022-08-25T13:47:43Z","timestamp":1661435263000},"page":"e0273208","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Effects of \u03b12-adrenoceptor stimulation on luminal alkalinisation and net fluid flux in rat duodenum"],"prefix":"10.1371","volume":"17","author":[{"given":"Olof","family":"Nylander","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Sj\u00f6blom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Sedin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5586-2906","authenticated-orcid":true,"given":"David","family":"Dahlgren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,8,25]]},"reference":[{"key":"pone.0273208.ref001","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/j.1365-2982.2009.01381.x","article-title":"The participation of the sympathetic innervation of the gastrointestinal tract in disease states.","volume":"22","author":"A Lomax","year":"2010","journal-title":"Neurogastroenterology & Motility"},{"key":"pone.0273208.ref002","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1056\/NEJM199604253341707","article-title":"The enteric nervous system","volume":"334","author":"RK Goyal","year":"1996","journal-title":"New England Journal of Medicine"},{"key":"pone.0273208.ref003","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1177\/1756283X15625586","article-title":"What about clonidine for diarrhoea? 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