{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T05:22:47Z","timestamp":1780550567212,"version":"3.54.1"},"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2022,8,25]],"date-time":"2022-08-25T00:00:00Z","timestamp":1661385600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Antimicrobial peptides may be alternatives to traditional antibiotics with reduced bacterial resistance. The antimicrobial peptide GL13K was derived from the salivary protein BPIFA2. This study determined the relative activity of the L-and D-enantiomers of GL13K to wild-type and drug-resistant strains of three gram-negative species and against<jats:italic>Pseudomonas aeruginosa<\/jats:italic>biofilms. DGL13K displayed in vitro activity against extended-spectrum beta-lactamase (ESBL)-producing and<jats:italic>Klebsiella pneumoniae<\/jats:italic>carbapenemase (KPC)-producing<jats:italic>Klebsiella pneumoniae<\/jats:italic>(MICs 16\u201332 \u03bcg\/ml), MDR and XDR<jats:italic>P<\/jats:italic>.<jats:italic>aeruginosa<\/jats:italic>, and XDR<jats:italic>Acinetobacter baumannii<\/jats:italic>carrying metallo-beta-lactamases (MICs 8\u201332 \u03bcg\/ml).<jats:italic>P<\/jats:italic>.<jats:italic>aeruginosa<\/jats:italic>showed low inherent resistance to DGL13K and the increased metabolic activity and growth caused by sub-MIC concentrations of GL13K peptides did not result in acquired bacterial resistance. Daily treatment for approximately two weeks did not increase the MIC of DGL13K or cause cross-resistance between LGL13K and DGL13K. These data suggest that DGL13K is a promising antimicrobial peptide candidate for further development.<\/jats:p>","DOI":"10.1371\/journal.pone.0273504","type":"journal-article","created":{"date-parts":[[2022,8,25]],"date-time":"2022-08-25T17:36:58Z","timestamp":1661449018000},"page":"e0273504","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":30,"title":["The antimicrobial peptide DGL13K is active against drug-resistant gram-negative bacteria and sub-inhibitory concentrations stimulate bacterial growth without causing resistance"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7855-4949","authenticated-orcid":true,"given":"Sven-Ulrik","family":"Gorr","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hunter V.","family":"Brigman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jadyn C.","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth B.","family":"Hirsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,8,25]]},"reference":[{"key":"pone.0273504.ref001","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1586\/14787210.5.6.951","article-title":"Alternative mechanisms of action of cationic antimicrobial peptides on bacteria","volume":"5","author":"JD Hale","year":"2007","journal-title":"Expert Rev Anti Infect Ther"},{"key":"pone.0273504.ref002","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1038\/s41564-018-0164-0","article-title":"Antibiotic-resistant bacteria show widespread collateral sensitivity to antimicrobial peptides","volume":"3","author":"V Lazar","year":"2018","journal-title":"Nat Microbiol"},{"key":"pone.0273504.ref003","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1111\/exd.12929","article-title":"Antimicrobial peptides and wound healing: biological and therapeutic considerations","volume":"25","author":"ML Mangoni","year":"2016","journal-title":"Exp Dermatol"},{"key":"pone.0273504.ref004","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1038\/415389a","article-title":"Antimicrobial peptides of multicellular organisms","volume":"415","author":"M. 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