{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T17:25:30Z","timestamp":1791739530817,"version":"4.3.4"},"reference-count":110,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T00:00:00Z","timestamp":1663718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fondos del Sistema FEES\/CONARE","award":["02-2020"],"award-info":[{"award-number":["02-2020"]}]},{"name":"Fondos FIDA, Universidad Nacional","award":["SIA 0047-17 to C. G-V"],"award-info":[{"award-number":["SIA 0047-17 to C. 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The two-component regulatory system (TCS) BvrR\/BvrS of\n                    <jats:italic>B<\/jats:italic>\n                    .\n                    <jats:italic>abortus<\/jats:italic>\n                    is conserved in members of the\n                    <jats:italic>Alphaproteobacteria<\/jats:italic>\n                    class. It is related to the expression of genes required for host interaction and intracellular survival. Here we report that\n                    <jats:italic>bvrR<\/jats:italic>\n                    and\n                    <jats:italic>bvrS<\/jats:italic>\n                    are part of an operon composed of 16 genes encoding functions related to nitrogen metabolism, DNA repair and recombination, cell cycle arrest, and stress response. Synteny of this genomic region within close\n                    <jats:italic>Alphaproteobacteria<\/jats:italic>\n                    members suggests a conserved role in coordinating the expression of carbon and nitrogen metabolic pathways. In addition, we performed a ChIP-Seq analysis after exposure of bacteria to conditions that mimic the intracellular environment. Genes encoding enzymes at metabolic crossroads of the pentose phosphate shunt, gluconeogenesis, cell envelope homeostasis, nucleotide synthesis, cell division, and virulence are BvrR\/BvrS direct targets. A 14 bp DNA BvrR binding motif was found and investigated in selected gene targets such as\n                    <jats:italic>virB1<\/jats:italic>\n                    ,\n                    <jats:italic>bvrR<\/jats:italic>\n                    ,\n                    <jats:italic>pckA<\/jats:italic>\n                    ,\n                    <jats:italic>omp25<\/jats:italic>\n                    , and\n                    <jats:italic>tamA<\/jats:italic>\n                    . Understanding gene expression regulation is essential to elucidate how\n                    <jats:italic>Brucella<\/jats:italic>\n                    orchestrates a physiological response leading to a furtive pathogenic strategy.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0274397","type":"journal-article","created":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T13:32:39Z","timestamp":1663767159000},"page":"e0274397","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":20,"title":["The regulon of Brucella abortus two-component system BvrR\/BvrS reveals the coordination of metabolic pathways required for intracellular life"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0990-149X","authenticated-orcid":true,"given":"Olga","family":"Rivas-Solano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathilde","family":"Van der Henst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Castillo-Zeled\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela","family":"Su\u00e1rez-Esquivel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5129-4879","authenticated-orcid":true,"given":"Lohendy","family":"Mu\u00f1oz-Vargas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2481-1057","authenticated-orcid":true,"given":"Zeuz","family":"Capitan-Barrios","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas R.","family":"Thomson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esteban","family":"Chaves-Olarte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8771-3959","authenticated-orcid":true,"given":"Edgardo","family":"Moreno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"De Bolle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1036-920X","authenticated-orcid":true,"given":"Caterina","family":"Guzm\u00e1n-Verri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,9,21]]},"reference":[{"key":"pone.0274397.ref001","doi-asserted-by":"crossref","first-page":"3569","DOI":"10.1128\/jb.172.7.3569-3576.1990","article-title":"Brucella abortus 16S rRNA and lipid A reveal a phylogenetic relationship with members of the alpha-2 subdivision of the class Proteobacteria","volume":"172","author":"E Moreno","year":"1990","journal-title":"J Bacteriol"},{"key":"pone.0274397.ref002","volume-title":"The Nature of Brucellosis","author":"WW 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