{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T13:49:02Z","timestamp":1762868942745,"version":"3.37.3"},"reference-count":24,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2022,10,4]],"date-time":"2022-10-04T00:00:00Z","timestamp":1664841600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["2018-01023"],"award-info":[{"award-number":["2018-01023"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["2018-01025"],"award-info":[{"award-number":["2018-01025"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Objectives<\/jats:title>\n<jats:p>Carbapenemase-producing <jats:italic>Enterobacterales<\/jats:italic> (CPE) are high priority targets of global antimicrobial surveillance. Herein, we determined the colonization rate of CPE on admission to intensive care units in Vientiane, Lao PDR in August-September 2019.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>Data regarding clinical conditions, infection control, and antibiotic usage were collected during admission. Rectal swab samples (n = 137) collected during admission were inoculated to selective chromogenic agars, followed by confirmatory tests for extended-spectrum beta-lactamases and carbapenemases. All CPE isolates were sequenced on Illumina (HiSeq2500), reads assembled using SPAdes 3.13, and the draft genomes used to query a database (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/www.genomicepidemiology.org\/\" xlink:type=\"simple\">https:\/\/www.genomicepidemiology.org<\/jats:ext-link>) for resistome, plasmid replicons, and sequence types (ST). Optical DNA mapping (ODM) was used to characterize plasmids and to determine location of resistance genes. Minimum spanning tree was generated using the Bacterial Isolate Genome Sequence database (BIGSdb) and annotated using iTOL.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Result<\/jats:title>\n<jats:p>From 47 <jats:italic>Enterobacterales<\/jats:italic> isolated on selective agars, <jats:italic>K<\/jats:italic>. <jats:italic>pneumoniae<\/jats:italic> (25\/47) and <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> (12\/47) were the most prevalent species, followed by <jats:italic>K aerogenes<\/jats:italic> (2\/47), <jats:italic>K<\/jats:italic>. <jats:italic>variicola<\/jats:italic> (1\/47), and <jats:italic>K<\/jats:italic>. <jats:italic>oxytoca<\/jats:italic> (1\/47). The overall prevalence of ESBLs was 51.0%; <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> 83.3% (10\/12) and <jats:italic>Klebsiella<\/jats:italic> spp. 41.3% (12\/29). Twenty percent of the <jats:italic>K<\/jats:italic>. <jats:italic>pneumoniae<\/jats:italic> (5\/25) isolates were carbapenem-resistant, and 4\/5 contained the <jats:italic>bla<\/jats:italic><jats:sub>NDM-1<\/jats:sub> gene. All <jats:italic>bla<\/jats:italic><jats:sub>NDM-1<\/jats:sub> isolates belonged to ST147 and were indistinguishable with cgMLST. ODM showed that the <jats:italic>bla<\/jats:italic><jats:sub>NDM-1<\/jats:sub> gene was located on identical plasmids in all isolates.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>The prevalence of ESBL-producing <jats:italic>Enterobacterales<\/jats:italic> was high, while carbapenemases were less common. However, the detection of clonal dissemination of <jats:italic>bla<\/jats:italic><jats:sub>NDM-1<\/jats:sub>-producing <jats:italic>K<\/jats:italic>. <jats:italic>pneumoniae<\/jats:italic> isolates in one of the intensive care units calls for vigilance. Stringent infection prevention and antimicrobial stewardship strategies are highly important measures.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0274419","type":"journal-article","created":{"date-parts":[[2022,10,4]],"date-time":"2022-10-04T17:25:51Z","timestamp":1664904351000},"page":"e0274419","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["Fecal carriage and clonal dissemination of blaNDM-1 carrying Klebsiella pneumoniae sequence type 147 at an intensive care unit in Lao PDR"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8295-2106","authenticated-orcid":true,"given":"Tsegaye","family":"Sewunet","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4661-242X","authenticated-orcid":true,"given":"Sriram","family":"K. K.","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ha Hoang","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noikaseumsy","family":"Sithivong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ngoc Thi Bich","family":"Hoang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanphanom","family":"Sychareun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kokasia","family":"Nengmongvang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mattias","family":"Larsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0046-6348","authenticated-orcid":true,"given":"Linus","family":"Olson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fredrik","family":"Westerlund","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian 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Chemother"},{"key":"pone.0274419.ref004","first-page":"1","article-title":"Global dissemination of carbapenemase-producing Klebsiella pneumoniae: Epidemiology, genetic context, treatment options, and detection methods","volume":"7","author":"CR Lee","year":"2016","journal-title":"Front Microbiol"},{"key":"pone.0274419.ref005","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1007\/s00284-018-1475-3","article-title":"Tracking Multidrug-Resistant Klebsiella pneumoniae from an Italian Hospital: Molecular Epidemiology and Surveillance by PFGE, RAPD and PCR-Based Resistance Genes Prevalence","volume":"75","author":"G Ripabelli","year":"2018","journal-title":"Curr Microbiol"},{"key":"pone.0274419.ref006","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-022-30637-5","article-title":"Whole genome sequencing reveals hidden transmission of carbapenemase-producing Enterobacterales","volume":"13","author":"K Marimuthu","year":"2022","journal-title":"Nat Commun"},{"key":"pone.0274419.ref007","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.eimc.2016.10.004","article-title":"Molecular epidemiology of carbapenemase-producing Enterobacteriaceae infection\/colonisation in a hospital in Madrid","volume":"36","author":"P Bra\u00f1as","year":"2018","journal-title":"Enferm Infecc Microbiol Clin"},{"key":"pone.0274419.ref008","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1080\/21505594.2016.1222343","article-title":"The global epidemiology of carbapenemase-producing Enterobacteriaceae","volume":"8","author":"D van Duin","year":"2017","journal-title":"Virulence"},{"key":"pone.0274419.ref009","first-page":"1","volume":"17","author":"T Tada","year":"2017","journal-title":"Dissemination of Carbapenem-resistant Klebsiella pneumoniae clinical isolates with various combinations of Carbapenemases 16S rRNA Methylases (RmtB and RmtC) in Vietnam"},{"key":"pone.0274419.ref010","doi-asserted-by":"crossref","first-page":"100531","DOI":"10.1016\/j.lanwpc.2022.100531","article-title":"Articles Evaluation of trends in hospital antimicrobial use in the Lao PDR using repeated point-prevalence surveys- evidence to improve treatment guideline use","volume":"27","author":"V Chansamouth","year":"2022","journal-title":"Lancet Reg Heal\u2014West Pacific"},{"key":"pone.0274419.ref011","doi-asserted-by":"crossref","first-page":"2804","DOI":"10.1093\/jac\/dkaa258","article-title":"Optical maps of plasmids as a proxy for clonal spread of MDR bacteria: A case study of an outbreak in a rural Ethiopian hospital","volume":"75","author":"YL Lin","year":"2020","journal-title":"J Antimicrob Chemother"},{"key":"pone.0274419.ref012","article-title":"High diversity of blaNDM-1-encoding plasmids in Klebsiella pneumoniae isolated from neonates in a Vietnamese hospital","volume":"106496","author":"KK Sriram","year":"2021","journal-title":"Int J Antimicrob Agents"},{"key":"pone.0274419.ref013","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1016\/j.bbrc.2011.11.128","article-title":"A single-step competitive binding assay for mapping of single DNA molecules","volume":"417","author":"LK Nyberg","year":"2012","journal-title":"Biochem Biophys Res Commun"},{"key":"pone.0274419.ref014","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1021\/ma5022067","article-title":"Nanoconfined Circular and Linear DNA: Equilibrium Conformations and Unfolding Kinetics","volume":"48","author":"M Alizadehheidari","year":"2015","journal-title":"Macromolecules"},{"key":"pone.0274419.ref015","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1039\/b912918a","article-title":"DNA in nanochannels-directly visualizing genomic information","volume":"39","author":"F Persson","year":"2010","journal-title":"Chem Soc 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