{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T18:24:07Z","timestamp":1761157447599,"version":"3.40.5"},"reference-count":30,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T00:00:00Z","timestamp":1665964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"crossref","award":["310030_200354"],"award-info":[{"award-number":["310030_200354"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>A 3-months old Chinese shar-pei puppy with ichthyosis was investigated. The dog showed generalized scaling, alopecia and footpad lesions. Histopathological examinations demonstrated a non-epidermolytic hyperkeratosis. The parents of the affected puppy did not show any skin lesions. A trio whole genome sequencing analysis identified a heterozygous <jats:italic>de novo<\/jats:italic> 3 bp deletion in the <jats:italic>KRT1<\/jats:italic> gene in the affected dog. This variant, NM_001003392.1:c.567_569del, is predicted to delete a single asparagine from the conserved coil 1A motif within the rod domain of KRT1, NP_001003392.1:p.(Asn190del). Immunohistochemistry demonstrated normal levels of KRT1 expression in the epidermis and follicular epithelia. This might indicate that the variant possibly interferes with keratin dimerization or another function of KRT1. Missense variants affecting the homologous asparagine residue of the human KRT1 cause epidermolytic hyperkeratosis. Histologically, the investigated Chinese shar-pei showed a non-epidermolytic ichthyosis. The finding of a <jats:italic>de novo<\/jats:italic> variant in an excellent functional candidate gene strongly suggests that KRT1:p.Asn190del caused the ichthyosis phenotype in the affected Chinese shar-pei. To the best of our knowledge, this is the first description of a <jats:italic>KRT1<\/jats:italic>-related non-epidermolytic ichthyosis in domestic animals.<\/jats:p>","DOI":"10.1371\/journal.pone.0275367","type":"journal-article","created":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T17:32:08Z","timestamp":1666027928000},"page":"e0275367","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["A de novo variant in the keratin 1 gene (KRT1) in a Chinese shar-pei dog with severe congenital cornification disorder and non-epidermolytic ichthyosis"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1118-9016","authenticated-orcid":true,"given":"Verena K.","family":"Affolter","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Kiener","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vidhya","family":"Jagannathan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terry","family":"Nagle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0553-4880","authenticated-orcid":true,"given":"Tosso","family":"Leeb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2022,10,17]]},"reference":[{"issue":"1","key":"pone.0275367.ref001","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.cvsm.2012.09.005","article-title":"Canine ichthyosis and related disorders of cornification","volume":"43","author":"EA Mauldin","year":"2013","journal-title":"Vet Clin North Am Small Anim Pract"},{"issue":"1","key":"pone.0275367.ref002","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1111\/j.1365-2133.2005.06735.x","article-title":"Mild recessive epidermolytic hyperkeratosis associated with a novel keratin 10 donor splice-site mutation in a family of Norfolk terrier dogs","volume":"153","author":"KM Credille","year":"2005","journal-title":"Br J Dermatol"},{"issue":"2","key":"pone.0275367.ref003","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1038\/ng.1056","article-title":"PNPLA1 mutations cause autosomal recessive congenital ichthyosis in golden retriever dogs and humans","volume":"44","author":"A Grall","year":"2012","journal-title":"Nat Genet"},{"issue":"6","key":"pone.0275367.ref004","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1016\/j.ajpath.2018.02.008","article-title":"Cellular and Metabolic Basis for the Ichthyotic Phenotype in NIPAL4 (Ichthyin)-Deficient Canines","volume":"188","author":"EA Mauldin","year":"2018","journal-title":"Am J Pathol"},{"issue":"2","key":"pone.0275367.ref005","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1111\/j.1365-2133.2009.09161.x","article-title":"Transglutaminase 1-deficient recessive lamellar ichthyosis associated with a LINE-1 insertion in Jack Russell terrier dogs","volume":"161","author":"KM Credille","year":"2009","journal-title":"Br J Dermatol"},{"issue":"4","key":"pone.0275367.ref006","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1177\/0300985814551425","article-title":"Autosomal Recessive Congenital Ichthyosis in American Bulldogs Is Associated With NIPAL4 (ICHTHYIN) Deficiency","volume":"52","author":"EA Mauldin","year":"2015","journal-title":"Vet Pathol"},{"issue":"1","key":"pone.0275367.ref007","doi-asserted-by":"crossref","first-page":"e0170708","DOI":"10.1371\/journal.pone.0170708","article-title":"A Defect in NIPAL4 Is Associated with Autosomal Recessive Congenital Ichthyosis in American Bulldogs","volume":"12","author":"ML Casal","year":"2017","journal-title":"PLoS One"},{"key":"pone.0275367.ref008","doi-asserted-by":"crossref","unstructured":"Kiener S, Wiener DJ, Hopke K, Diesel AB, Jagannathan V, Maulsin EA, editors. 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