{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T13:20:00Z","timestamp":1773235200008,"version":"3.50.1"},"reference-count":32,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2022,10,3]],"date-time":"2022-10-03T00:00:00Z","timestamp":1664755200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004410","name":"Scientific and Technological Research Council of Turkey","doi-asserted-by":"crossref","award":["118Z765"],"award-info":[{"award-number":["118Z765"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004410","name":"Scientific and Technological Research Council of Turkey","doi-asserted-by":"crossref","award":["216Z006"],"award-info":[{"award-number":["216Z006"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>DEK has a short isoform (DEK isoform-2; DEK2) that lacks amino acid residues between 49\u201382. The full-length DEK (DEK isoform-1; DEK1) is ubiquitously expressed and plays a role in different cellular processes but whether DEK2 is involved in these processes remains elusive. We stably overexpressed DEK2 in human bone marrow stromal cell line HS-27A, in which endogenous DEKs were intact or suppressed via short hairpin RNA (sh-RNA). We have found that contrary to ectopic DEK1, DEK2 locates in the nucleus and nucleolus, causes persistent \u03b3H2AX signal upon doxorubicin treatment, and couldn\u2019t functionally compensate for the loss of DEK1. In addition, DEK2 overexpressing cells were more sensitive to doxorubicin than DEK1-cells. Expressions of <jats:italic>DEK1<\/jats:italic> and <jats:italic>DEK2<\/jats:italic> in cell lines and primary tumors exhibit tissue specificity. <jats:italic>DEK1<\/jats:italic> is upregulated in cancers of the colon, liver, and lung compared to normal tissues while both <jats:italic>DEK1<\/jats:italic> and <jats:italic>DEK2<\/jats:italic> are downregulated in subsets of kidney, prostate, and thyroid carcinomas. Interestingly, only <jats:italic>DEK2<\/jats:italic> was downregulated in a subset of breast tumors suggesting that <jats:italic>DEK2<\/jats:italic> can be modulated differently than <jats:italic>DEK1<\/jats:italic> in specific cancers. In summary, our findings show distinct expression patterns and subcellular location and suggest non-overlapping functions between the two DEK isoforms.<\/jats:p>","DOI":"10.1371\/journal.pone.0275476","type":"journal-article","created":{"date-parts":[[2022,10,3]],"date-time":"2022-10-03T17:38:11Z","timestamp":1664818691000},"page":"e0275476","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["Doxorubicin induces prolonged DNA damage signal in cells overexpressing DEK 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