{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T03:29:39Z","timestamp":1790306979767,"version":"4.1.0"},"reference-count":64,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T00:00:00Z","timestamp":1668470400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"the Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["Research Project for Practical Applications of Regenerative Medicine"],"award-info":[{"award-number":["Research Project for Practical Applications of Regenerative Medicine"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"name":"RegeNephro Co., Ltd"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Cell therapies using human induced pluripotent stem cell (hiPSC)-derived nephron progenitor cells (NPCs) are expected to ameliorate acute kidney injury (AKI). However, using hiPSC-derived NPCs clinically is a challenge because hiPSCs themselves are tumorigenic. <jats:italic>LIN28A<\/jats:italic>, <jats:italic>ESRG<\/jats:italic>, <jats:italic>CNMD<\/jats:italic> and <jats:italic>SFRP2<\/jats:italic> transcripts have been used as a marker of residual hiPSCs for a variety of cell types undergoing clinical trials. In this study, by reanalyzing public databases, we found a baseline expression of <jats:italic>LIN28A<\/jats:italic>, <jats:italic>ESRG<\/jats:italic>, <jats:italic>CNMD<\/jats:italic> and <jats:italic>SFRP2<\/jats:italic> in hiPSC-derived NPCs and several other cell types, suggesting <jats:italic>LIN28A<\/jats:italic>, <jats:italic>ESRG<\/jats:italic>, <jats:italic>CNMD<\/jats:italic> and <jats:italic>SFRP2<\/jats:italic> are not always reliable markers for iPSC detection. As an alternative, we discovered a lncRNA marker gene, <jats:italic>MIR302CHG<\/jats:italic>, among many known and unknown iPSC markers, as highly differentially expressed between hiPSCs and NPCs, by RNA sequencing and quantitative RT-PCR (qRT-PCR) analyses. Using <jats:italic>MIR302CHG<\/jats:italic> as an hiPSC marker, we constructed two assay methods, a combination of magnetic bead-based enrichment and qRT-PCR and digital droplet PCR alone, to detect a small number of residual hiPSCs in NPC populations. The use of these <jats:italic>in vitro<\/jats:italic> assays could contribute to patient safety in treatments using hiPSC-derived cells.<\/jats:p>","DOI":"10.1371\/journal.pone.0275600","type":"journal-article","created":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T18:26:25Z","timestamp":1668536785000},"page":"e0275600","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":13,"title":["In vitro methods to ensure absence of residual undifferentiated human induced pluripotent stem cells intermingled in induced nephron progenitor 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