{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T07:17:51Z","timestamp":1782631071300,"version":"3.54.5"},"reference-count":40,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T00:00:00Z","timestamp":1665619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Resveratrol may improve organ dysfunction after experimental hemorrhagic or septic shock, and some of these effects appear to be mediated by estrogen receptors. However, the influence of resveratrol on liver function and hepatic microcirculation after hemorrhagic shock is unknown, and a presumed mediation via estrogen receptors has not been investigated in this context.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>Male Sprague-Dawley rats (200-300g, n = 14\/group) underwent hemorrhagic shock for 90 min (MAP 35\u00b15 mmHg) and were resuscitated with shed blood and Ringer\u2019s solution. Animals were treated intravenously with vehicle (1% EtOH), resveratrol (0.2 mg\/kg), the unselective estrogen receptor antagonist ICI 182,780 (0.05 mg\/kg) or resveratrol + ICI 182,780 prior to retransfusion. Sham-operated animals did not undergo hemorrhage but were treated likewise. After 2 hours of reperfusion, liver function was assessed either by plasma disappearance rate of indocyanine green (PDR<jats:sub>ICG<\/jats:sub>) or evaluation of hepatic perfusion and hepatic integrity by intravital microscopy, serum enzyme as well as cytokine levels.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>Compared to vehicle controls, administration of resveratrol significantly improved PDR<jats:sub>ICG<\/jats:sub>, hepatic perfusion index and hepatic integrity after hemorrhagic shock. The co-administration of ICI 182,780 completely abolished the protective effect only with regard to liver function.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusions<\/jats:title>\n<jats:p>This study shows that resveratrol may improve liver function and hepatocellular integrity after hemorrhagic shock in rats; estrogen receptors mediate these effects at least partially.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0275632","type":"journal-article","created":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T17:36:04Z","timestamp":1665682564000},"page":"e0275632","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["Resveratrol therapy improves liver function via estrogen-receptors after hemorrhagic shock in rats"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8875-586X","authenticated-orcid":true,"given":"Alexander","family":"Wolf","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Fink","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jochen","family":"Hinkelbein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thilo","family":"Mertke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Volk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Mathes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,10,13]]},"reference":[{"issue":"9","key":"pone.0275632.ref001","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1002\/lt.22117","article-title":"Liver ischemia\/reperfusion injury: processes in inflammatory networks\u2014a review","volume":"16","author":"M Abu-Amara","year":"2010","journal-title":"Liver Transpl"},{"issue":"1","key":"pone.0275632.ref002","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1097\/shk.0b013e3180644ca3","article-title":"Melatonin pretreatment improves liver function and hepatic perfusion after hemorrhagic shock","volume":"29","author":"AM Mathes","year":"2008","journal-title":"Shock"},{"issue":"10","key":"pone.0275632.ref003","doi-asserted-by":"crossref","first-page":"2863","DOI":"10.1097\/CCM.0b013e318187b863","article-title":"Selective activation of melatonin receptors with ramelteon improves liver function and hepatic perfusion after hemorrhagic shock in rat","volume":"36","author":"AM Mathes","year":"2008","journal-title":"Crit Care Med"},{"issue":"1","key":"pone.0275632.ref004","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1097\/01.CCM.0000292088.33318.F0","article-title":"Melatonin receptors mediate improvements of liver function but not of hepatic perfusion and integrity after hemorrhagic shock in rats","volume":"36","author":"AM Mathes","year":"2008","journal-title":"Crit Care Med"},{"issue":"8","key":"pone.0275632.ref005","article-title":"Antioxidant Activity of Graptopetalum paraguayense E. 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