{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:01:55Z","timestamp":1787022115940,"version":"build-2736575974"},"reference-count":62,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T00:00:00Z","timestamp":1666137600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"BBSRC Norwich Research Park Doctoral Training Studentship","award":["BB\/M011216\/1"],"award-info":[{"award-number":["BB\/M011216\/1"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Phytases, enzymes that degrade phytate present in feedstuffs, are widely added to the diets of monogastric animals. Many studies have correlated phytase addition with improved animal productivity and a subset of these have sought to correlate animal performance with phytase-mediated generation of inositol phosphates in different parts of the gastro-intestinal tract or with release of inositol or of phosphate, the absorbable products of phytate degradation. Remarkably, the effect of dietary phytase on tissue inositol phosphates has not been studied. The objective of this study was to determine effect of phytase supplementation on liver and kidney\n                    <jats:italic>myo<\/jats:italic>\n                    -inositol and\n                    <jats:italic>myo<\/jats:italic>\n                    -inositol phosphates in broiler chickens. For this, methods were developed to measure inositol phosphates in chicken tissues. The study comprised wheat\/soy-based diets containing one of three levels of phytase (0, 500 and 6,000 FTU\/kg of modified\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>coli<\/jats:italic>\n                    6-phytase). Diets were provided to broilers for 21 D and on day 21 digesta were collected from the gizzard and ileum. Liver and kidney tissue were harvested.\n                    <jats:italic>Myo<\/jats:italic>\n                    -inositol and inositol phosphates were measured in diet, digesta, liver and kidney. Gizzard and ileal content inositol was increased progressively, and total inositol phosphates reduced progressively, by phytase supplementation. The predominant higher inositol phosphates detected in tissues, D-and\/or L-Ins(3,4,5,6)P\n                    <jats:sub>4<\/jats:sub>\n                    and Ins(1,3,4,5,6)P\n                    <jats:sub>5<\/jats:sub>\n                    , differed from those (D-and\/or L-Ins(1,2,3,4)P\n                    <jats:sub>4<\/jats:sub>\n                    , D-and\/or L-Ins(1,2,5,6)P\n                    <jats:sub>4<\/jats:sub>\n                    , Ins(1,2,3,4,6)P\n                    <jats:sub>5<\/jats:sub>\n                    , D-and\/or L-Ins(1,2,3,4,5)P\n                    <jats:sub>5<\/jats:sub>\n                    and D-and\/or L-Ins(1,2,4,5,6)P\n                    <jats:sub>5<\/jats:sub>\n                    ) generated from phytate (InsP\n                    <jats:sub>6<\/jats:sub>\n                    ) degradation by\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>coli<\/jats:italic>\n                    6-phytase or endogenous feed phytase, suggesting tissue inositol phosphates are not the result of direct absorption. Kidney inositol phosphates were reduced progressively by phytase supplementation. These data suggest that tissue inositol phosphate concentrations can be influenced by dietary phytase inclusion rate and that such effects are tissue specific, though the consequences for physiology of such changes have yet to be elucidated.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0275742","type":"journal-article","created":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T13:33:13Z","timestamp":1666186393000},"page":"e0275742","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["Phytase dose-dependent response of kidney inositol phosphate levels in poultry"],"prefix":"10.1371","volume":"17","author":[{"given":"Colleen","family":"Sprigg","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayley","family":"Whitfield","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Burton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawn","family":"Scholey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5308-4290","authenticated-orcid":true,"given":"Michael R.","family":"Bedford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6179-9109","authenticated-orcid":true,"given":"Charles A.","family":"Brearley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,10,19]]},"reference":[{"issue":"6","key":"pone.0275742.ref001","doi-asserted-by":"crossref","first-page":"1082","DOI":"10.1590\/0103-8478cr20150801","article-title":"Endogenous losses and true digestibility of phosphorus in rice bran with or without phytase determined with piglets","volume":"46","author":"W Lucca","year":"2016","journal-title":"Ci\u00eancia Rural"},{"issue":"4","key":"pone.0275742.ref002","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1093\/ps\/77.4.557","article-title":"Phytase Activity in the Small Intestinal Brush Border Membrane of the Chicken","volume":"77","author":"DD Maenz","year":"1998","journal-title":"Poult Sci"},{"issue":"1\u20132","key":"pone.0275742.ref003","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.anifeedsci.2006.06.010","article-title":"Microbial phytase in poultry nutrition","volume":"135","author":"PH Selle","year":"2007","journal-title":"Anim Feed Sci Technol"},{"issue":"February","key":"pone.0275742.ref004","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.anifeedsci.2017.01.008","article-title":"A systematic view on the effect of phytase on ileal amino acid digestibility in broilers","volume":"225","author":"AJ Cowieson","year":"2017","journal-title":"Anim Feed Sci Technol"},{"issue":"3","key":"pone.0275742.ref005","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1111\/jpn.12482","article-title":"Exploratory transcriptomic analysis in muscle tissue of broilers fed a phytase-supplemented diet","volume":"101","author":"J Schmeisser","year":"2017","journal-title":"J Anim Physiol Anim Nutr (Berl)"},{"issue":"October","key":"pone.0275742.ref006","first-page":"1","article-title":"Quantum Blue reduces the severity of Woody Breast Myopathy via modulation of oxygen homeostasis-related genes in broiler chickens","volume":"10","author":"E Greene","year":"2019","journal-title":"Front Physiol"},{"issue":"8","key":"pone.0275742.ref007","doi-asserted-by":"crossref","first-page":"2124","DOI":"10.3382\/ps.2013-03140","article-title":"The effect of microbial phytase and myo-inositol on performance and blood biochemistry of broiler chickens fed wheat\/corn-based diets","volume":"92","author":"AJ Cowieson","year":"2013","journal-title":"Poult Sci"},{"issue":"11","key":"pone.0275742.ref008","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1017\/S0007114517003026","article-title":"Time-series responses of swine plasma metabolites to ingestion of diets containing myo-inositol or phytase","volume":"118","author":"AJ Cowieson","year":"2017","journal-title":"Br J Nutr"},{"issue":"1","key":"pone.0275742.ref009","doi-asserted-by":"crossref","first-page":"260","DOI":"10.3382\/ps\/pey341","article-title":"Manipulation of plasma myo-inositol in broiler chickens: effect on growth performance, dietary energy, nutrient availability and hepatic function","volume":"98","author":"V Pirgozliev","year":"2019","journal-title":"Poult Sci"},{"issue":"1","key":"pone.0275742.ref010","doi-asserted-by":"crossref","first-page":"199","DOI":"10.3382\/ps.2012-02651","article-title":"Effect of inositol and phytases on hematological indices and \u03b1-1 acid glycoprotein levels in laying hens fed phosphorus-deficient corn-soybean meal-based diets","volume":"92","author":"K Zy\u0142a","year":"2013","journal-title":"Poult Sci"},{"issue":"4","key":"pone.0275742.ref011","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.3382\/ps\/pex392","article-title":"Effect of phytase on growth performance, phytate degradation and gene expression of myo-inositol transporters in the small intestine, liver and kidney of 21 day old broilers","volume":"97","author":"CL Walk","year":"2018","journal-title":"Poult Sci"},{"issue":"12","key":"pone.0275742.ref012","doi-asserted-by":"crossref","first-page":"6797","DOI":"10.1016\/j.psj.2020.08.064","article-title":"Phytate degradation, myo-inositol release, and utilization of phosphorus and calcium by two strains of laying hens in five production periods","volume":"99","author":"V Sommerfeld","year":"2020","journal-title":"Poult Sci"},{"issue":"12","key":"pone.0275742.ref013","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1017\/S0007114518000934","article-title":"Phosphorus absorption and gene expression levels of related transporters in the small intestine of broilers","volume":"119","author":"Y Hu","year":"2018","journal-title":"Br J Nutr"},{"issue":"10","key":"pone.0275742.ref014","doi-asserted-by":"crossref","first-page":"4914","DOI":"10.1016\/j.psj.2020.06.030","article-title":"Interactive effect of dietary calcium and phytase on broilers challenged with subclinical necrotic enteritis: part 2. Gut permeability, phytate ester concentrations, jejunal gene expression, and intestinal morphology","volume":"99","author":"HK Zanu","year":"2020","journal-title":"Poult Sci"},{"issue":"June","key":"pone.0275742.ref015","first-page":"1","article-title":"Monitoring phytate hydrolysis using serial blood sampling and feather myo-inositol levels in broilers","volume":"11","author":"E Greene","year":"2020","journal-title":"Front Physiol"},{"issue":"1","key":"pone.0275742.ref016","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-021-84169-x","article-title":"Interrelationship of myo-inositol pathways with systemic metabolic conditions in two strains of high-performance laying hens during their productive life span","volume":"11","author":"F Gonzalez-Uarquin","year":"2021","journal-title":"Sci Rep"},{"issue":"3","key":"pone.0275742.ref017","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1017\/S1751731119002337","article-title":"Dietary phytase and myo-inositol supplementation are associated with distinct plasma metabolome profile in broiler chickens","volume":"14","author":"F Gonzalez-Uarquin","year":"2020","journal-title":"Animal"},{"issue":"August","key":"pone.0275742.ref018","first-page":"1","article-title":"Muscle metabolome profiles in Woody Breast-(un)affected broilers: Effects of Quantum Blue phytase-enriched diet","volume":"7","author":"E Greene","year":"2020","journal-title":"Front Vet Sci"},{"issue":"5","key":"pone.0275742.ref019","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.3382\/ps\/pev065","article-title":"Modulation of small intestinal phosphate transporter by dietary supplements of mineral phosphorus and phytase in broilers","volume":"94","author":"K Huber","year":"2015","journal-title":"Poult Sci"},{"key":"pone.0275742.ref020","article-title":"Effect of phytase supplementation on plasma and organ myo-inositol content and erythrocyte inositol phosphate in chickens","author":"H Whitfield","year":"2022","journal-title":"J Appl Anim Nutr"},{"issue":"5","key":"pone.0275742.ref021","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.3382\/ps\/pev087","article-title":"Interactions between supplemented mineral phosphorus and phytase on phytate hydrolysis and inositol phosphates in the small intestine of broilers","volume":"94","author":"E Zeller","year":"2015","journal-title":"Poult Sci"},{"issue":"7","key":"pone.0275742.ref022","doi-asserted-by":"crossref","first-page":"142","DOI":"10.2527\/jas.2015-9762","article-title":"The effects of phytase on grower pig growth performance and ileal inositol phosphate degradation","volume":"94","author":"S Laird","year":"2016","journal-title":"J Anim Sci"},{"issue":"3","key":"pone.0275742.ref023","doi-asserted-by":"crossref","first-page":"100899","DOI":"10.1016\/j.psj.2020.11.068","article-title":"Inositol and gradient phytase supplementation in broiler diets during a 6-week production period: 2. Effects on phytate degradation and inositol liberation in gizzard and ileal digesta contents","volume":"100","author":"R Kriseldi","year":"2021","journal-title":"Poult Sci"},{"issue":"5","key":"pone.0275742.ref024","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.3382\/ps.2013-03571","article-title":"Influence of superdoses of a novel microbial phytase on growth performance, tibia ash, and gizzard phytate and inositol in young broilers","volume":"93","author":"CL Walk","year":"2014","journal-title":"Poult Sci"},{"issue":"2","key":"pone.0275742.ref025","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1016\/j.psj.2019.10.016","article-title":"Phytase dosing affects phytate degradation and Muc2 transporter gene expression in broiler starters","volume":"99","author":"KM Ajuwon","year":"2020","journal-title":"Poult Sci"},{"issue":"4","key":"pone.0275742.ref026","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.3382\/ps\/pex404","article-title":"Interactive effects of phosphorus, calcium, and phytase supplements on products of phytate degradation in the digestive tract of broiler chickens","volume":"97","author":"V Sommerfeld","year":"2018","journal-title":"Poult Sci"},{"issue":"2","key":"pone.0275742.ref027","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1016\/j.psj.2019.10.014","article-title":"Myo-inositol: its metabolism and potential implications for poultry nutrition\u2014a review","volume":"99","author":"F Gonzalez-Uarquin","year":"2020","journal-title":"Poult Sci"},{"issue":"1","key":"pone.0275742.ref028","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1006\/abio.2001.5449","article-title":"A straightforward method for analysis of highly phosphorylated inositols in blood cells by high-performance liquid chromatography","volume":"300","author":"I Casals","year":"2002","journal-title":"Anal Biochem"},{"issue":"2","key":"pone.0275742.ref029","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1042\/bj2540585","article-title":"A novel metal-dye detection system permits picomolar-range h.p.l.c. analysis of inositol polyphosphates from non-radioactively labelled cell or tissue specimens","volume":"254","author":"GW Mayr","year":"1988","journal-title":"Biochem J"},{"issue":"14","key":"pone.0275742.ref030","doi-asserted-by":"crossref","first-page":"2621","DOI":"10.1042\/BCJ20200423","article-title":"An ATP-responsive metabolic cassette comprised of inositol tris\/tetrakisphosphate kinase 1 (ITPK1) and inositol pentakisphosphate 2-kinase (IPK1) buffers diphosphosphoinositol phosphate level","volume":"477","author":"H Whitfield","year":"2020","journal-title":"Biochem J"},{"issue":"11","key":"pone.0275742.ref031","doi-asserted-by":"crossref","first-page":"5972","DOI":"10.1016\/j.psj.2020.08.045","article-title":"Research Note: Jejunum phosphatases and systemic myo-inositol in broiler chickens fed without or with supplemented phytase","volume":"99","author":"F Gonzalez-Uarquin","year":"2020","journal-title":"Poult Sci"},{"issue":"3","key":"pone.0275742.ref032","doi-asserted-by":"crossref","first-page":"150014","DOI":"10.1098\/rsob.150014","article-title":"A novel method for the purification of inositol phosphates from biological samples reveals that no phytate is present in human plasma or urine","volume":"5","author":"MSC Wilson","year":"2015","journal-title":"Open Biol"},{"issue":"2","key":"pone.0275742.ref033","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1042\/BJ20081417","article-title":"Do mammals make all their own inositol hexakisphosphate?","volume":"416","author":"AJ Letcher","year":"2008","journal-title":"Biochem J"},{"issue":"1","key":"pone.0275742.ref034","first-page":"1","article-title":"Analysis of inositol phosphate metabolism by capillary electrophoresis electrospray ionization mass spectrometry","volume":"11","author":"D Qiu","year":"2020","journal-title":"Nat Commun"},{"issue":"7","key":"pone.0275742.ref035","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1111\/j.1476-5381.2010.00872.x","article-title":"Animal research: Reporting in vivo experiments: The ARRIVE guidelines","volume":"160","author":"C Kilkenny","year":"2010","journal-title":"Br J Pharmacol"},{"issue":"1\u20132","key":"pone.0275742.ref036","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1007\/s11104-017-3315-9","article-title":"Simple synthesis of 32P-labelled inositol hexakisphosphates for study of phosphate transformations","volume":"427","author":"H Whitfield","year":"2018","journal-title":"Plant Soil"},{"issue":"1","key":"pone.0275742.ref037","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/0003-2697(88)90374-0","article-title":"Gradient ion chromatography of inositol phosphates","volume":"175","author":"BQ Phillippy","year":"1988","journal-title":"Anal Biochem"},{"issue":"5","key":"pone.0275742.ref038","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1080\/00071668.2018.1496403","article-title":"Exogenous phytase and xylanase exhibit opposing effects on real-time gizzard pH in broiler chickens","volume":"59","author":"SA Lee","year":"2018","journal-title":"Br Poult Sci"},{"issue":"4","key":"pone.0275742.ref039","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1111\/1751-7915.13733","article-title":"Improved sensitivity, accuracy and prediction provided by a high-performance liquid chromatography screen for the isolation of phytase-harbouring organisms from environmental samples","volume":"14","author":"GD Rix","year":"2021","journal-title":"Microb Biotechnol"},{"issue":"3","key":"pone.0275742.ref040","doi-asserted-by":"crossref","first-page":"920","DOI":"10.3382\/ps\/pex390","article-title":"Influence of phytase or myo-inositol supplements on performance and phytate degradation products in the crop, ileum, and blood of broiler chickens","volume":"97","author":"V Sommerfeld","year":"2018","journal-title":"Poult Sci"},{"issue":"3","key":"pone.0275742.ref041","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1042\/bj2800631","article-title":"Masses of inositol phosphates in resting and tetanically stimulated vertebrate skeletal muscles","volume":"280","author":"GW Mayr","year":"1991","journal-title":"Biochem J"},{"key":"pone.0275742.ref042","first-page":"435","article-title":"Product-precursor relationships amongst inositol polyphosphates","volume":"265","author":"LR Stephens","year":"1990","journal-title":"Biochem J"},{"issue":"6","key":"pone.0275742.ref043","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1146\/annurev.nu.06.070186.003023","article-title":"Metabolism and function of phospholipids","author":"BJ Holub","year":"1986","journal-title":"Annu Rev Nutr"},{"issue":"2","key":"pone.0275742.ref044","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/0003-9861(67)90357-8","article-title":"Metabolism of myo-lnositol in animals","volume":"118","author":"CF Howard","year":"1967","journal-title":"Arch Biochem Biophys"},{"issue":"2","key":"pone.0275742.ref045","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.semnephrol.2012.12.018","article-title":"Management of Natural and Added Dietary Phosphorus Burden in Kidney Disease HHS Public Access Author manuscript","volume":"33","author":"A Cupisti","year":"2013","journal-title":"Semin Nephrol"},{"issue":"4","key":"pone.0275742.ref046","doi-asserted-by":"crossref","first-page":"620","DOI":"10.4103\/2230-8210.113752","article-title":"Renal phosphate handling","volume":"17","author":"N Prasad","year":"2013","journal-title":"Physiology. Indian J Endocrinol Metab"},{"key":"pone.0275742.ref047","first-page":"126","article-title":"Evidence for FGF23 involvement in a bone-kidney axis regulating bone mineralization and systemic phosphate and vitamin D homeostasis","volume":"728","author":"A Martin","year":"2012","journal-title":"Adv Exp Med Biol"},{"issue":"4","key":"pone.0275742.ref048","doi-asserted-by":"crossref","first-page":"2317","DOI":"10.1152\/physrev.00022.2017","article-title":"Phosphate as a signaling molecule and its sensing mechanism","volume":"98","author":"T Michigami","year":"2018","journal-title":"Physiol Rev"},{"issue":"3 57\u20133","key":"pone.0275742.ref049","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1152\/ajprenal.00097.2004","article-title":"Internalization of renal type IIc Na-Pi cotransporter in response to a high-phosphate diet","volume":"288","author":"H Segawa","year":"2005","journal-title":"Am J Physiol\u2014Ren Physiol"},{"issue":"10","key":"pone.0275742.ref050","doi-asserted-by":"crossref","first-page":"4896","DOI":"10.3382\/ps\/pez239","article-title":"Effect of anti-fibroblast growth factor 23 antibody on phosphate and calcium metabolism in adenine gavaged laying hens","volume":"98","author":"Z Ren","year":"2019","journal-title":"Poult Sci"},{"key":"pone.0275742.ref051","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/0003-2697(81)90344-4","article-title":"Quantitation of vitamin D and its metabolites and their plasma concentrations in five species of animals","volume":"116","author":"RL Horst","year":"1981","journal-title":"Anal Biochem"},{"issue":"10","key":"pone.0275742.ref052","doi-asserted-by":"crossref","first-page":"1930","DOI":"10.1093\/jn\/110.10.1930","article-title":"Absorption and excretion of cholecalciferol and of 25-hydroxycholecalciferol and metabolites in birds","volume":"110","author":"A Bar","year":"1980","journal-title":"J Nutr"},{"issue":"8","key":"pone.0275742.ref053","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.3382\/ps.2011-02129","article-title":"Vitamin D-1\u03b1-hydroxylase and vitamin D-24-hydroxylase mRNA studies in chickens","volume":"91","author":"R Shanmugasundaram","year":"2012","journal-title":"Poult Sci"},{"issue":"5","key":"pone.0275742.ref054","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/cdn\/nzab018","article-title":"Implications of vitamin D research in chickens can advance human nutrition and perspectives for the future","volume":"5","author":"MF Warren","year":"2021","journal-title":"Curr Dev Nutr"},{"issue":"June","key":"pone.0275742.ref055","first-page":"1","article-title":"Efficacy of 1-\u03b1-hydroxycholecalciferol supplementation in young broiler feed suggests reducing calcium levels at grower phase","volume":"7","author":"MF Warren","year":"2020","journal-title":"Front Vet Sc"},{"issue":"1","key":"pone.0275742.ref056","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1093\/ps\/76.1.37","article-title":"Utilization of phytate phosphorus and calcium as influenced by microbial phytase, cholecalciferol, and the calcium:total phosphorus ratio in broiler diets","volume":"76","author":"H Qian","year":"1997","journal-title":"Poult Sci"},{"issue":"3","key":"pone.0275742.ref057","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1080\/00071669008417290","article-title":"Effects of dietary calcium, available phosphorus and vitamin d on growth rate, food utilisation, plasma and bone constituents and calcium and phosphorus retention of commercial broiler strains","volume":"31","author":"TM Shafey","year":"1990","journal-title":"Br Poult Sci"},{"issue":"2","key":"pone.0275742.ref058","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1079\/BJN19910029","article-title":"The effects of dietary levels of inorganic phosphorus, calcium and cholecalciferol on the digestibility of phytate-P by the chick","volume":"66","author":"A Mohammed","year":"1991","journal-title":"Br J Nutr"},{"issue":"2","key":"pone.0275742.ref059","doi-asserted-by":"crossref","first-page":"288","DOI":"10.3382\/ps.0730288","article-title":"Quantitative requirement for cholecalciferol in the absence of ultraviolet light","volume":"73","author":"HM Edwards","year":"1994","journal-title":"Poult Sci"},{"issue":"1","key":"pone.0275742.ref060","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1079\/BJN19580007","article-title":"The effect of high and low levels of dietary inorganic phosphate on the pre-laying storage of calcium and phosphorus and on the composition of the medullary and cortical bone in pullets","volume":"12","author":"TG Taylor","year":"1958","journal-title":"Br J Nutr"},{"key":"pone.0275742.ref061","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1038\/172504a0","article-title":"Avian Medullary Bone","volume":"172","author":"TG Taylor","year":"1953","journal-title":"Nature"},{"key":"pone.0275742.ref062","first-page":"1","article-title":"Kinase controls circulating phosphate in mammals","author":"Y Moritoh","year":"2021","journal-title":"Nat Commun"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0275742","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T13:34:38Z","timestamp":1666186478000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0275742"}},"subtitle":[],"editor":[{"given":"Juan J.","family":"Loor","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,10,19]]},"references-count":62,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2022,10,19]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0275742","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,19]]}}}