{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T12:47:40Z","timestamp":1766407660394,"version":"3.37.3"},"reference-count":98,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T00:00:00Z","timestamp":1665619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"crossref","award":["Innovation Project grant [69864]"],"award-info":[{"award-number":["Innovation Project grant [69864]"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004156","name":"Mahidol University","doi-asserted-by":"publisher","award":["a New Discovery and Frontier Research Grant FY2022"],"award-info":[{"award-number":["a New Discovery and Frontier Research Grant FY2022"]}],"id":[{"id":"10.13039\/501100004156","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004156","name":"Mahidol University","doi-asserted-by":"publisher","award":["Postdoctoral fellowships"],"award-info":[{"award-number":["Postdoctoral fellowships"]}],"id":[{"id":"10.13039\/501100004156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Schistosomiasis is a neglected tropical disease caused by an infection of the parasitic flatworms schistosomes. <jats:italic>Schistosoma mekongi<\/jats:italic> is a restricted <jats:italic>Schistosoma<\/jats:italic> species found near the Mekong River, mainly in southern Laos and northern Cambodia. Because there is no vaccine or effective early diagnosis available for <jats:italic>S<\/jats:italic>. <jats:italic>mekongi<\/jats:italic>, additional biomarkers are required. In this study, serum biomarkers associated with <jats:italic>S<\/jats:italic>. <jats:italic>mekongi-<\/jats:italic>infected mice were identified at 14-, 28-, 42-, and 56-days post-infection. Circulating proteins and antigens of <jats:italic>S<\/jats:italic>. <jats:italic>mekongi<\/jats:italic> in mouse sera were analyzed using mass spectrometry-based proteomics. <jats:bold>Serine<\/jats:bold> protease inhibitors and macrophage erythroblast attacher were down-regulated in mouse sera at all infection timepoints. In addition, 54 circulating proteins and 55 antigens of <jats:italic>S<\/jats:italic>. <jats:italic>mekongi<\/jats:italic> were identified. Notable circulating proteins included kyphoscoliosis peptidase and putative tuberin, and antigens were detected at all four infection timepoints, particularly in the early stages (12 days). The putative tuberin sequence of <jats:italic>S<\/jats:italic>. <jats:italic>mekongi<\/jats:italic> was highly similar to homologs found in other members of the genus Schistosoma and less similar to human and murine sequences. Our study provided the identity of promising diagnostic biomarkers that could be applicable in early schistosomiasis diagnosis and vaccine development.<\/jats:p>","DOI":"10.1371\/journal.pone.0275992","type":"journal-article","created":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T17:42:31Z","timestamp":1665682951000},"page":"e0275992","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera"],"prefix":"10.1371","volume":"17","author":[{"given":"Naphatsamon","family":"Uthailak","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Poom","family":"Adisakwattana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tipparat","family":"Thiangtrongjit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanin","family":"Limpanont","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phiraphol","family":"Chusongsang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yupa","family":"Chusongsang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanthi","family":"Tanasarnprasert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1154-6485","authenticated-orcid":true,"given":"Onrapak","family":"Reamtong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2022,10,13]]},"reference":[{"issue":"9541","key":"pone.0275992.ref001","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1016\/S0140-6736(06)69440-3","article-title":"Human schistosomiasis.","volume":"368","author":"B Gryseels","year":"2006","journal-title":"Lancet"},{"issue":"7","key":"pone.0275992.ref002","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/S1473-3099(06)70521-7","article-title":"Schistosomiasis and water resources development: systematic review, meta-analysis, and estimates of people at risk.","volume":"6","author":"P Steinmann","year":"2006","journal-title":"Lancet Infect. 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