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Studies have shown that insecticide resistance affects vector competence (VC) of some mosquito species. This study evaluates the effect of resistance to lambda-cyhalothrin and <jats:italic>kdr<\/jats:italic> V1016I mutation genotypes on the VC of <jats:italic>Ae<\/jats:italic>. <jats:italic>aegypti<\/jats:italic> strains for DENV-2, ZIKV, and CHIKV. Three <jats:italic>Ae<\/jats:italic>. <jats:italic>aegypti<\/jats:italic> strains with gradual lambda-cyhalothrin resistance (susceptible, resistant, and highly resistant) were infected with DENV-2, ZIKV, and CHIKV. Individual mosquitoes were tested to detect virus infection in the abdomen and head-salivary glands, using RT-PCR, and genotypes for V1016I mutations using allele-specific PCR. Recorded VC variables were midgut infection rate (MIR), dissemination rate (DIR), and dissemination efficiency (DIE). Lambda-cyhalothrin resistance affects differentially VC variables for ZIKV, DENV-2, and CHIKV. For ZIKV, an apparent gradual increase in DIR and DIE with the increase in insecticide resistance was observed. For DENV-2 the MIR and DIE were higher in insecticide resistant strains. For CHIKV, only MIR could be evaluated, this variable was higher in insecticide resistance strains. The presence of <jats:italic>kdr<\/jats:italic> V1016I mutation on mosquito resistant strains did not affect VC variables for three study viruses.<\/jats:p>","DOI":"10.1371\/journal.pone.0276493","type":"journal-article","created":{"date-parts":[[2022,10,25]],"date-time":"2022-10-25T17:31:35Z","timestamp":1666719095000},"page":"e0276493","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Vector competence of lambda-cyhalothrin resistant Aedes aegypti strains for dengue-2, Zika and chikungunya viruses in Colombia"],"prefix":"10.1371","volume":"17","author":[{"given":"Idalba M.","family":"Serrato","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Moreno-Aguilera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola A.","family":"Caicedo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yenifer","family":"Orobio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0119-5025","authenticated-orcid":true,"given":"Clara B.","family":"Ocampo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald","family":"Maestre-Serrano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dioselina","family":"Pel\u00e1ez-Carvajal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5153-8383","authenticated-orcid":true,"given":"Martha L.","family":"Ahumada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,10,25]]},"reference":[{"key":"pone.0276493.ref001","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.pt.2017.12.004","article-title":"How Do Virus\u2013Mosquito Interactions Lead to Viral Emergence?","volume":"34","author":"C R\u00fcckert","year":"2018","journal-title":"Trends Parasitol"},{"key":"pone.0276493.ref002","doi-asserted-by":"crossref","first-page":"e08347","DOI":"10.7554\/eLife.08347","article-title":"The global distribution of the arbovirus vectors Aedes aegypti and Ae. 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