{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T07:47:15Z","timestamp":1777276035929,"version":"3.51.4"},"reference-count":44,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2022,10,21]],"date-time":"2022-10-21T00:00:00Z","timestamp":1666310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Thermophilic <jats:italic>Campylobacter<\/jats:italic> species are common cause of animal and human bacterial diseases with growing resistance to antimicrobials. The aim of this study was to determine the prevalence and antimicrobial susceptibility pattern of <jats:italic>Campylobacter<\/jats:italic> species from bovine, knives and personnel in Jimma Town, Ethiopia. Faecal samples and carcasses swabs were collected from cattle systematically selected from the annual plan of Jimma Municipal Abattoir. Personnel hand and knife swabs were collected after slaughtering each selected cattle. A cross-sectional study with systematic sampling method was conducted from October 2019 to September 2020 for the isolation, identification and antimicrobial susceptibility pattern of thermophilic <jats:italic>Campylobacter<\/jats:italic> species. Isolation and identification of <jats:italic>Campylobacter<\/jats:italic> species were performed according to the techniques recommended by the International Organization for Standardization, and <jats:italic>in vitro<\/jats:italic> antibiotic susceptibility testing was screened using the standard agar disc diffusion method as recommended by Clinical and Laboratory Standards Institutions. A total of 684 samples (171 samples from faeces, carcasses, knives and personnel hands, were collected independently). The overall prevalence of thermophilic <jats:italic>Campylobacter<\/jats:italic>species was 5.6% (38\/684). Majority of the isolates were from faecal samples (12.9%, n = 22) followed by carcass swabs(4.1% n = 7), knife swabs(3.5% n = 6) and personnel hand swabs(1.8% n = 3). Isolated and identified species of <jats:italic>C<\/jats:italic>.<jats:italic>jejuni<\/jats:italic>, <jats:italic>C<\/jats:italic>. <jats:italic>coli<\/jats:italic> and <jats:italic>C<\/jats:italic>. <jats:italic>lari<\/jats:italic> accounted for 63.2%, 23.7% and 13.2%, respectively. The isolated <jats:italic>Campylobacter<\/jats:italic> species were found to be resistant to Cephalothin (100%), Ampicillin (60.5%), Cefotaxime (60.5%), Chloramphenicol (47.4%) and Tetracycline (42.1%). On the other hand, the isolates were susceptible to Nalidixic acid (86.8%), Ciprofloxacin (86.8%), Sulphamethazole (84.2%), Ceftriaxone (78.9%), Clindamycin (68.4%) and Cefixime (65.8%). 84.2% of the isolates showed multi-drug resistance for three-to-six drug classes. All the <jats:italic>C<\/jats:italic>. <jats:italic>lari<\/jats:italic> isolates were multidrug resistant. All the three isolated species of <jats:italic>Campylobacter<\/jats:italic> were resistant to Cephalothin, and most were multidrug resistant. Isolation of <jats:italic>Campylobacter<\/jats:italic> species from faecal, carcass, knife and hand swabs revealed possible risk of contamination and exposure to Campylobacter infection of those who consume raw meat. Therefore, enactment of hygienic practices during the slaughtering process, proper handling and cooking of meat and awareness creation on jurisdictional antibiotic usage are required to avoid <jats:italic>Campylobacter<\/jats:italic> infection.<\/jats:p>","DOI":"10.1371\/journal.pone.0276625","type":"journal-article","created":{"date-parts":[[2022,10,21]],"date-time":"2022-10-21T17:26:59Z","timestamp":1666373219000},"page":"e0276625","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":21,"title":["Isolation, identification and antibiotic resistance profile of thermophilic Campylobacter species from Bovine, Knives and personnel at Jimma Town Abattoir, Ethiopia"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3182-9810","authenticated-orcid":true,"given":"Motuma","family":"Debelo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nezif","family":"Mohammed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abebaw","family":"Tiruneh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadele","family":"Tolosa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2022,10,21]]},"reference":[{"key":"pone.0276625.ref001","volume-title":"Sherris Medical Microbiology: Medical Microbiology","author":"KJ Ryan","year":"2014","edition":"6"},{"issue":"0799","key":"pone.0276625.ref002","first-page":"1","article-title":"Zoonotic and Public Health Implications of Campylobacter Species and Squamates (Lizards, Snakes and Amphisbaenians)","volume":"9","author":"NM Masila","year":"2020","journal-title":"Pathogens"},{"key":"pone.0276625.ref003","unstructured":"WHO. 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