{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T02:55:01Z","timestamp":1780455301780,"version":"3.54.1"},"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The effects of congenital Zika syndrome (CZS) on the tooth development of infected children are not well known. The aim of this study was to analyze the association of CZS with dental alterations in children with microcephaly seen at a referral hospital in Rio Grande do Norte, Brazil. The chronology and sequence of tooth eruption and the presence of dental alterations were evaluated by a single calibrated examiner (kappa &gt; 0.80) in 62 children aged 7 to 35 months with microcephaly associated with CZS and other congenital infections. Medical data of the mother and child were collected from the records and the parents responded to a socioeconomic questionnaire. Descriptive analysis and Fisher\u2019s exact test were used (5% significance level). The mean age of the children was 26.4 months (SD = 7.52). The mean weight and head circumference at birth were 2,593 g (SD = 0.60) and 29.6 cm (SD = 2.48), respectively. Microcephaly was associated with congenital Zika virus infection in 79% of cases and with other congenital infections in 21%. No significant association was found between CZS and alterations in the chronology (p = 1.00) or sequence of tooth eruption (p = 0.16) or changes in enamel development (p = 1.00). In conclusion, children with microcephaly exhibit a delay and alterations in the sequence of tooth eruption of primary teeth, as well as developmental defects of enamel, which are not associated with Zika virus infection.<\/jats:p>","DOI":"10.1371\/journal.pone.0276931","type":"journal-article","created":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T17:23:26Z","timestamp":1667323406000},"page":"e0276931","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["Association of congenital Zika syndrome with dental alterations in children with microcephaly"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6856-226X","authenticated-orcid":true,"given":"Patr\u00edcia N\u00f3brega","family":"Gomes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz Aguiar","family":"do Amaral","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelita Duarte","family":"Azevedo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haline Cunha","family":"de Medeiros Maia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nivia Maria Rodrigues","family":"Arrais","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenio Costa","family":"de Lima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,11,1]]},"reference":[{"issue":"5","key":"pone.0276931.ref001","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/0035-9203(52)90042-4","article-title":"Zika virus isolations and serological specificity","volume":"46","author":"GW Dick","year":"1952","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"24","key":"pone.0276931.ref002","doi-asserted-by":"crossref","first-page":"2536","DOI":"10.1056\/NEJMoa0805715","article-title":"Zika virus outbrealk on Yao Islands, Federated States os Micronesia","volume":"360","author":"MR Duffy","year":"2009","journal-title":"N Engl J Med"},{"issue":"1","key":"pone.0276931.ref003","first-page":"151","article-title":"Complications associated with Zika Virus Infection: a systematic review study","volume":"24","author":"U Faluyi","year":"2016","journal-title":"Am Sci Res J Eng Technol Sci"},{"issue":"5","key":"pone.0276931.ref004","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1097\/QCO.0000000000000301","article-title":"Zika virus infection: epidemiology, clinical manifestations and diagnosis.","volume":"29","author":"GA Calvet","year":"2016","journal-title":"Curr Opin Infect Dis"},{"issue":"1","key":"pone.0276931.ref005","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1093\/bmb\/ldw023","article-title":"Zika virus: a review for clinicians","volume":"119","author":"Y Mo","year":"2016","journal-title":"Br Med Bull"},{"key":"pone.0276931.ref006","unstructured":"BRASIL. Minist\u00e9rio da Sa\u00fade. Secretaria de Vigil\u00e2ncia em Sa\u00fade. V\u00edrus Zika no Brasil: a resposta do SUS. Bras\u00edlia: Minist\u00e9rio da Sa\u00fade; 2017. Dispon\u00edvel em: http:\/\/bvsms.saude.gov.br\/bvs\/publicacoes\/virus_zika_brasil_resposta_sus.pdf. Acesso em 14 mar. 2018."},{"key":"pone.0276931.ref007","volume-title":"Epidemia do v\u00edrus Zika e microcefalia no Brasil: emerg\u00eancia, evolu\u00e7\u00e3o e enfrentamento.","author":"LP Garcia","year":"2018"},{"issue":"1","key":"pone.0276931.ref008","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1002\/uog.15831","article-title":"Zika virus intrauterine infection causes fetal brain abnormality and microcephaly: tip of the iceberg? Physician Alert.","volume":"47","author":"Oliveira Mas","year":"2016","journal-title":"Ultrasound Obstet Gynecol."},{"issue":"10030","key":"pone.0276931.ref009","doi-asserted-by":"crossref","first-page":"1811","DOI":"10.1016\/S0140-6736(16)30253-7","article-title":"Positive IgM for Zika virus in the cerebrospinal fluid of 30 neonates with microcephaly in Brazil","volume":"387","author":"MT Cordeiro","year":"2016","journal-title":"Lancet"},{"key":"pone.0276931.ref010","volume-title":"Minist\u00e9rio da Sa\u00fade. Secretaria de Vigil\u00e2ncia em Sa\u00fade. Protocolo de Vigil\u00e2ncia e resposta \u00e0 ocorr\u00eancia de microcefalia e\/ou altera\u00e7\u00f5es do Sistema Nervoso Central (SNC). 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Secretaria de Vigil\u00e2ncia em Sa\u00fade. Secretaria de Aten\u00e7\u00e3o \u00e0 Sa\u00fade. Orienta\u00e7\u00f5es integradas de vigil\u00e2ncia e aten\u00e7\u00e3o \u00e0 sa\u00fade no \u00e2mbito da Emerg\u00eancia de Sa\u00fade P\u00fablica de Import\u00e2ncia Nacional: procedimentos para o monitoramento das altera\u00e7\u00f5es no crescimento e desenvolvimento a partir da gesta\u00e7\u00e3o at\u00e9 a primeira inf\u00e2ncia, relacionadas \u00e0 infec\u00e7\u00e3o pelo v\u00edrus Zika e outras etiologias infeciosas dentro da capacidade operacional do SUS","author":"BRASIL","year":"2017"},{"issue":"10","key":"pone.0276931.ref015","doi-asserted-by":"crossref","first-page":"e0223408","DOI":"10.1371\/journal.pone.0223408","article-title":"Congenital Zika Syndrome in a Brazil- Paraguay-Bolivia border region: clinical features of cases diagnosed between 2015 and 2018.","volume":"14","author":"FA Venancio","year":"2019","journal-title":"PLoS ONE."},{"issue":"12","key":"pone.0276931.ref016","doi-asserted-by":"crossref","first-page":"e0242367","DOI":"10.1371\/journal.pone.0242367","article-title":"Congenital Zika Syndrome: a systematic review.","volume":"15","author":"DA Freitas","year":"2020","journal-title":"PLoS ONE."},{"key":"pone.0276931.ref017","first-page":"70","volume-title":"Ten Cate\u2019s Oral Histology","author":"A. 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Commission on Oral Health Research and Epidemiology.","volume":"42","author":"Report of on FDI Working Group","year":"1992","journal-title":"Int Dent J"},{"issue":"3","key":"pone.0276931.ref022","doi-asserted-by":"crossref","first-page":"345","DOI":"10.4103\/0976-237X.214553","article-title":"Challenges of dental care for children with microcephaly carrying Zika congenital syndrome.","volume":"8","author":"AL Cavalcanti","year":"2017","journal-title":"Contemp Clin Dent"},{"key":"pone.0276931.ref023","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1111\/ipd.12402","article-title":"Alterations in the primary teeth of children with microcephaly in Northeast Brazil: a comparative study.","volume":"28","author":"RMP Siqueira","year":"2018","journal-title":"Int J Paediatr Dent.,"},{"issue":"1","key":"pone.0276931.ref024","first-page":"e3982","article-title":"Chronology of the first deciduous tooth eruption in Brazilian children with microcephaly associated with zika virus: a 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D\u2019Agostino","year":"2020","journal-title":"Spec Care Dentist."},{"key":"pone.0276931.ref028","doi-asserted-by":"crossref","first-page":"104598","DOI":"10.1016\/j.archoralbio.2019.104598","article-title":"Dental development in children born to Zikv -infected mothers: a case-based study","volume":"110","author":"Arnaud MA Silva MCPMD","year":"2020","journal-title":"Arch Oral Biol"},{"issue":"4","key":"pone.0276931.ref029","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1590\/S1678-77572006000400003","article-title":"The influence of gestational age and birth weight of the newborn on tooth eruption.","volume":"14","author":"SRP Ramos","year":"2006","journal-title":"J Appl Oral Sci"},{"issue":"1","key":"pone.0276931.ref030","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1111\/j.1740-8709.2008.00156.x","article-title":"Relationship between socio-demographic and anthropometric variables and number of erupted primary teeth in suburban Nigerian 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dental caries in deciduous and permanent dentition in Indaiatuba, S\u00e3o Paulo, Brazil.","volume":"23","author":"RMS Hoffmann","year":"2007","journal-title":"Cad Saude Publica"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0276931","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T17:23:59Z","timestamp":1667323439000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0276931"}},"subtitle":[],"editor":[{"given":"J. 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