{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T19:32:04Z","timestamp":1783452724335,"version":"3.55.0"},"reference-count":30,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Taiwan-Australian National University Scholarship"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    is an emerging nosocomial, opportunistic pathogen with growing clinical significance globally.\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>baumannii<\/jats:italic>\n                    has an exceptional ability to rapidly develop drug resistance. It is frequently responsible for ventilator-associated pneumonia in clinical settings and inflammation resulting in severe sepsis. The inflammatory response is mediated by host pattern-recognition receptors and the inflammasomes. Inflammasome activation triggers inflammatory responses, including the secretion of the pro-inflammatory cytokines IL-1\u03b2 and IL-18, the recruitment of innate immune effectors against\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>baumannii<\/jats:italic>\n                    infection, and the induction programmed cell death by pyroptosis. An important knowledge gap is how variation among clinical isolates affects the host\u2019s innate response and activation of the inflammasome during\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>baumannii<\/jats:italic>\n                    infection. In this study, we compared nine\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>baumannii<\/jats:italic>\n                    strains, including clinical locally-acquired isolates, in their ability to induce activation of the inflammasome and programmed cell death in primary macrophages, epithelial lung cell line and mice. We found a variation in survival outcomes of mice and bacterial dissemination in organs among three commercially available\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>baumannii<\/jats:italic>\n                    strains, likely due to the differences in virulence between strains. Interestingly, we found variability among\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>baumannii<\/jats:italic>\n                    strains in activation of the NLRP3 inflammasome, non-canonical Caspase-11 pathway, plasmatic secretion of the pro-inflammatory cytokine IL-1\u03b2 and programmed cell death. Our study highlights the importance of utilising multiple bacterial strains and clinical isolates with different virulence to investigate the innate immune response to\n                    <jats:italic>A<\/jats:italic>\n                    .\n                    <jats:italic>baumannii<\/jats:italic>\n                    infection.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0277019","type":"journal-article","created":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T13:27:02Z","timestamp":1667309222000},"page":"e0277019","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Differential activation of NLRP3 inflammasome by Acinetobacter baumannii strains"],"prefix":"10.1371","volume":"17","author":[{"given":"Fei-Ju","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lora","family":"Starrs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anukriti","family":"Mathur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hikari","family":"Ishii","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si Ming","family":"Man","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7434-926X","authenticated-orcid":true,"given":"Gaetan","family":"Burgio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,11,1]]},"reference":[{"issue":"9","key":"pone.0277019.ref001","article-title":"Tug of war between Acinetobacter baumannii and host immune responses","volume":"76","author":"F.J. 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