{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T09:47:02Z","timestamp":1784368022670,"version":"3.55.0"},"reference-count":36,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T00:00:00Z","timestamp":1668038400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    In mice, CD9 expression on the egg is required for efficient sperm-egg fusion and no effects on ovulation or male fertility are observed in CD9 null animals. Here we show that\n                    <jats:italic>cd9b<\/jats:italic>\n                    knockout zebrafish also appear to have fertility defects. In contrast to mice, fewer eggs were laid by\n                    <jats:italic>cd9b<\/jats:italic>\n                    knockout zebrafish pairs and, of the eggs laid, a lower percentage were fertilised. These effects could not be linked to primordial germ cell numbers or migration as these were not altered in the\n                    <jats:italic>cd9b<\/jats:italic>\n                    mutants. The decrease in egg numbers could be rescued by exchanging either\n                    <jats:italic>cd9b<\/jats:italic>\n                    knockout partner, male or female, for a wildtype partner. However, the fertilisation defect was only rescued by crossing a\n                    <jats:italic>cd9b<\/jats:italic>\n                    knockout female with a wildtype male. To exclude effects of mating behaviour we analysed clutch size and fertilisation using\n                    <jats:italic>in vitro<\/jats:italic>\n                    fertilisation techniques. Number of eggs and fertilisation rates were significantly reduced in the\n                    <jats:italic>cd9b<\/jats:italic>\n                    mutants suggesting the fertility defects are not solely due to courtship behaviours. Our results indicate that CD9 plays a more complex role in fish fertility than in mammals, with effects in both males and females.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0277274","type":"journal-article","created":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T13:31:56Z","timestamp":1668087116000},"page":"e0277274","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Tetraspanin Cd9b plays a role in fertility in zebrafish"],"prefix":"10.1371","volume":"17","author":[{"given":"Sarah","family":"Greaves","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8004-5908","authenticated-orcid":true,"given":"Katherine S.","family":"Marsay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4637-3059","authenticated-orcid":true,"given":"Peter N.","family":"Monk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry","family":"Roehl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2098-9747","authenticated-orcid":true,"given":"Lynda J.","family":"Partridge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,11,10]]},"reference":[{"issue":"3","key":"pone.0277274.ref001","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1042\/BST20160284","article-title":"Molecular interactions shaping the tetraspanin web","volume":"45","author":"SJ van Deventer","year":"2017","journal-title":"Biochem Soc Trans"},{"issue":"April","key":"pone.0277274.ref002","first-page":"1","article-title":"Tetraspanin CD9: A Key Regulator of Cell Adhesion in the Immune System","volume":"9","author":"R Reyes","year":"2018","journal-title":"Front Immunol"},{"key":"pone.0277274.ref003","first-page":"279","volume":"8408","author":"J Jankovi\u010dov\u00e1","year":"2015","journal-title":"Role of Tetraspanin CD9 Molecule in Fertilization of Mammals"},{"issue":"19","key":"pone.0277274.ref004","doi-asserted-by":"crossref","first-page":"3732","DOI":"10.1242\/dev.111534","article-title":"Binding of sperm protein Izumo1 and its egg receptor Juno drives Cd9 accumulation in the intercellular contact area prior to fusion during mammalian fertilization","volume":"141","author":"M Chalbi","year":"2014","journal-title":"Development"},{"key":"pone.0277274.ref005","article-title":"Egg CD9 protein tides correlated with sperm oscillations tune the gamete fusion ability in mammal. 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