{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:22:24Z","timestamp":1791404544272,"version":"4.3.3"},"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2023,1,17]],"date-time":"2023-01-17T00:00:00Z","timestamp":1673913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005734","name":"Connecticut Space Grant Consortium","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100005734","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100005734","name":"Connecticut Space Grant Consortium","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100005734","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100005734","name":"Connecticut Space Grant Consortium","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100005734","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100005734","name":"Connecticut Space Grant Consortium","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100005734","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Connecticut State University - American Association of University Professors"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Scorpions represent an ancient lineage of arachnids that have radiated across the globe and are incredibly resilient\u2014since some thrive in harsh environments and can exist on minimal and intermittent feedings. Given the emerging importance of microbiomes to an organism\u2019s health, it is intriguing to suggest that the long-term success of the scorpion bauplan may be linked to the microbiome. Little is known about scorpion microbiomes, and what is known, concentrates on the gut. The microbiome is not limited to the gut, rather it can be found within tissues, fluids and on external surfaces. We tested whether the scorpion telson, the venom-producing organ, of two species, <jats:italic>Smeringurus mesaensis<\/jats:italic> and <jats:italic>Hadrurus arizonensis<\/jats:italic>, contain bacteria. We isolated telson DNA from each species, amplified bacterial 16S rRNA genes, and identified the collection of bacteria present within each scorpion species. Our results show for the first time that telsons of non-buthid scorpion species do indeed contain bacteria. Interestingly, each scorpion species has a phylogenetically unique telson microbiome including Mollicutes symbionts. This study may change how we view scorpion biology and their venoms.<\/jats:p>","DOI":"10.1371\/journal.pone.0277303","type":"journal-article","created":{"date-parts":[[2023,1,18]],"date-time":"2023-01-18T03:59:28Z","timestamp":1674014368000},"page":"e0277303","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["A first molecular characterization of the scorpion telson microbiota of Hadrurus arizonensis and Smeringurus mesaensis"],"prefix":"10.1371","volume":"18","author":[{"given":"Christopher","family":"Shimwell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren","family":"Atkinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew 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Microbiol."},{"key":"pone.0277303.ref007","doi-asserted-by":"crossref","first-page":"575372","DOI":"10.3389\/fmicb.2020.575372","article-title":"To the land and beyond: Crab microbiomes as a paradigm for the evolution of terrestrialization","volume":"11","author":"S Cannicci","year":"2020","journal-title":"Front Microbiol"},{"key":"pone.0277303.ref008","first-page":"183","article-title":"The first record for Wolbachia in a scorpion: The parthenogenetic yellow scorpion Tityus serrulatus (Scorpiones, Buthidae).","volume":"14","author":"L Suesdek-Rocha","year":"2006","journal-title":"Revista Iberica de Arachnologia"},{"issue":"85","key":"pone.0277303.ref009","first-page":"19802","article-title":"Molecular diagnosis of Wolbachia endosymbiont from Iranian scorpion Hemiscorpius lepturus using polymerase chain reaction (PCR) amplification of 16 S rDNA gene.","volume":"10","author":"M Baradaran","year":"2011","journal-title":"Afr J 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adaptation to venom is common in snakes and spiders.","author":"E Esmaeilishirazifard","year":"2018","journal-title":"bioRxiv"},{"issue":"3","key":"pone.0277303.ref023","doi-asserted-by":"crossref","first-page":"e0240821","DOI":"10.1128\/spectrum.02408-21","article-title":"Bacterial adaptation to venom in snakes and arachnida.","volume":"10","author":"E Esmaeilishirazifard","year":"2022","journal-title":"Microbiol Spectr"},{"issue":"2","key":"pone.0277303.ref024","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1071\/IS19033","article-title":"Another one bites the dust: Taxonomic sampling of a key genus in phylogenomic datasets reveals more non-monophyletic groups in traditional scorpion classification.","volume":"34","author":"CE Santib\u00e1\u00f1ez-L\u00f3pez","year":"2020","journal-title":"Invertebrate Systematics."},{"issue":"2","key":"pone.0277303.ref025","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1128\/AEM.64.2.795-799.1998","article-title":"Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA","volume":"64","author":"JR Marchesi","year":"1998","journal-title":"Appl Environ Microbiol"},{"issue":"3","key":"pone.0277303.ref026","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"SF Altschul","year":"1990","journal-title":"J Mol Biol"},{"issue":"2","key":"pone.0277303.ref027","doi-asserted-by":"crossref","DOI":"10.1128\/mSphereDirect.00073-17","article-title":"OptiClust, an improved method for assigning amplicon-based sequence data to operational taxonomic units.","volume":"2","author":"SL Westcott","year":"2017","journal-title":"mSphere"},{"issue":"1","key":"pone.0277303.ref028","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1093\/nar\/gkg039","article-title":"The Ribosomal Database Project (RDP-II): Previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy.","volume":"31","author":"JR Cole","year":"2003","journal-title":"Nucleic Acids Res"},{"issue":"Database issue","key":"pone.0277303.ref029","first-page":"D590","article-title":"The SILVA ribosomal RNA gene database project: Improved data processing and web-based tools","volume":"41","author":"C Quast","year":"2013","journal-title":"Nucleic Acids Res"},{"key":"pone.0277303.ref030","volume-title":"Structural RNA homology search and alignment using covariance models","author":"Washington University in St. Louis","year":"2009"},{"issue":"1","key":"pone.0277303.ref031","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1093\/molbev\/msu300","article-title":"IQ-TREE: A fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies","volume":"32","author":"LT Nguyen","year":"2015","journal-title":"Mol Biol 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Biosci"},{"issue":"3","key":"pone.0277303.ref036","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1099\/00207713-47-3-763","article-title":"Spiroplasma platyhelix sp. nov., a new mollicute with unusual morphology and genome size from the dragonfly Pachydiplax longipennis","volume":"47","author":"DL Williamson","year":"1997","journal-title":"Int J Syst Bacteriol"},{"key":"pone.0277303.ref037","first-page":"132","article-title":"A new male-killing parasitism: Spiroplasma bacteria infect the ladybird beetle Anisosticta novemdecimpunctata (Coleoptera: Coccinellidae).","author":"MC Tinsley","year":"2006","journal-title":"Parasitology"},{"issue":"1","key":"pone.0277303.ref038","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1099\/00207713-45-1-23","article-title":"Spiroplasma ixodetis sp. nov., a new species from Ixodes pacificus ticks collected in Oregon","volume":"45","author":"JG Tully","year":"1995","journal-title":"Int J Syst Bacteriol"},{"key":"pone.0277303.ref039","doi-asserted-by":"crossref","first-page":"13699","DOI":"10.1038\/ncomms13699","article-title":"Amphibian gut microbiota shifts differentially in community structure but converges on habitat-specific predicted functions","volume":"7","author":"MC Bletz","year":"2016","journal-title":"Nat Commun"},{"issue":"17","key":"pone.0277303.ref040","doi-asserted-by":"crossref","first-page":"1630","DOI":"10.1080\/14786410903003780","article-title":"Bacterial production of tetrodotoxin in the pufferfish Arothron hispidus.","volume":"23","author":"S Campbell","year":"2009","journal-title":"Nat Prod Res"},{"issue":"6833","key":"pone.0277303.ref041","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1038\/35075148","article-title":"Protein function. Chaperonin turned insect toxin","volume":"411","author":"N Yoshida","year":"2001","journal-title":"Nature"},{"issue":"1","key":"pone.0277303.ref042","article-title":"The enigmatic genome of an obligate ancient Spiroplasma symbiont in a hadal holothurian","volume":"84","author":"LS He","year":"2018","journal-title":"Appl Environ Microbiol"},{"key":"pone.0277303.ref043","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.toxicon.2014.06.006","article-title":"Antimicrobial peptides from scorpion venoms","volume":"88","author":"PL Harrison","year":"2014","journal-title":"Toxicon"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0277303","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,18]],"date-time":"2023-01-18T04:00:12Z","timestamp":1674014412000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0277303"}},"subtitle":[],"editor":[{"given":"Michael Scott","family":"Brewer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,1,17]]},"references-count":43,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,1,17]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0277303","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,17]]}}}