{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T11:00:02Z","timestamp":1793012402723,"version":"4.5.0"},"reference-count":31,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2023,1,20]],"date-time":"2023-01-20T00:00:00Z","timestamp":1674172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PUE 22920160100101CO"],"award-info":[{"award-number":["PUE 22920160100101CO"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Recent preclinical studies have demonstrated that bone marrow (BM)-derived Muse cells have a homing mechanism to reach damaged cardiac tissue while also being able to reduce myocardial infarct size and improve cardiac function; however, the potential of BM-Muse cells to foster new blood-vessel formation has not been fully assessed. Up to date, adipose tissue (AT)-derived Muse cells remain to be studied in acute myocardial infarction (AMI). The aim of the present study was to analyze <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> the neovascularization capacity of AT-Muse cells while exploring their biodistribution and differentiation potential in a translational ovine model of AMI.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods and results<\/jats:title>\n<jats:p>AT-Muse cells were successfully isolated from ovine adipose tissue. In adult sheep, one or more diagonal branches of the left anterior descending coronary artery were permanently ligated for thirty minutes. Sheep were randomized in two groups and treated with intramyocardial injections: Vehicle (PBS, n = 4) and AT-Muse (2x10<jats:sup>7<\/jats:sup> AT-Muse cells labeled with PKH26 Red Fluorescent Dye, n = 4). Molecular characterization showed higher expression of angiogenic genes (<jats:italic>VEGF<\/jats:italic>, <jats:italic>PGF<\/jats:italic> and <jats:italic>ANG)<\/jats:italic> and increased number of tube formation in AT-Muse cells group compared to Adipose-derived mesenchymal stromal cells (ASCs) group. At 7 days post-IAM, the AT-Muse group showed significantly more arterioles and capillaries than the Vehicle group. Co-localization of PKH26+ cells with desmin, sarcomeric actin and troponin T implied the differentiation of Muse cells to a cardiac fate; moreover, PKH26+ cells also co-localized with a lectin marker, suggesting a possible differentiation to a vascular lineage.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>Intramyocardially administered AT-Muse cells displayed a significant neovascularization activity and survival capacity in an ovine model of AMI.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0277442","type":"journal-article","created":{"date-parts":[[2023,1,20]],"date-time":"2023-01-20T18:57:57Z","timestamp":1674241077000},"page":"e0277442","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Promoting early neovascularization by allotransplanted adipose-derived Muse cells in an ovine model of acute myocardial infarction"],"prefix":"10.1371","volume":"18","author":[{"given":"Martha G.","family":"Castillo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom\u00e1s M.","family":"Peralta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Locatelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Candela","family":"Velazquez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yamila","family":"Herrero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto J.","family":"Crottogini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda D.","family":"Olea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5960-4333","authenticated-orcid":true,"given":"Luis A.","family":"Cuniberti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,1,20]]},"reference":[{"issue":"25","key":"pone.0277442.ref001","doi-asserted-by":"crossref","first-page":"2982","DOI":"10.1016\/j.jacc.2020.11.010","article-title":"Global Burden of Cardiovascular Diseases and Risk Factors, 1990\u20132019","volume":"76","author":"G Roth","year":"2020","journal-title":"Journal of the American College of Cardiology"},{"issue":"10","key":"pone.0277442.ref002","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1038\/s41569-018-0036-6","article-title":"Therapeutic approaches for cardiac regeneration and repair","volume":"15","author":"H Hashimoto","year":"2018","journal-title":"Nature Reviews Cardiology"},{"issue":"8","key":"pone.0277442.ref003","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1161\/CIRCRESAHA.116.303614","article-title":"Use of Mesenchymal Stem Cells for Therapy of Cardiac Disease","volume":"116","author":"V Karantalis","year":"2015","journal-title":"Circulation Research"},{"issue":"7","key":"pone.0277442.ref004","doi-asserted-by":"crossref","first-page":"1610","DOI":"10.1016\/j.ymthe.2018.05.009","article-title":"Mesenchymal Stem Cell-Based Therapy for Cardiovascular Disease: Progress and Challenges","volume":"26","author":"L Bagno","year":"2018","journal-title":"Molecular Therapy"},{"issue":"3","key":"pone.0277442.ref005","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1152\/physrev.00019.2015","article-title":"Rebuilding the Damaged Heart: Mesenchymal Stem Cells, Cell-Based Therapy, and Engineered Heart Tissue","volume":"96","author":"S Golpanian","year":"2016","journal-title":"Physiological Reviews"},{"key":"pone.0277442.ref006","first-page":"100118","article-title":"Dare to dream? 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