{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T05:29:17Z","timestamp":1769837357235,"version":"3.49.0"},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2022,11,23]],"date-time":"2022-11-23T00:00:00Z","timestamp":1669161600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Consistently deciding its current extent and chance elements of tuberculosis (TB) in all levels of clinical settings contributes to the anticipation and control exertion of the disease. In Ethiopia, updated information is still needed at every healthcare level and in different risk groups to monitor the national program\u2019s performance, which aims to attain the 2035 goal. Hence, this study aimed to generate additional evidence data on the magnitude of <jats:italic>Mycobacterium tuberculosis<\/jats:italic> using the Gene Xpert assay among TB-suspected patients at Mizan-Tepi university teaching hospital, southwest Ethiopia.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>A cross-sectional descriptive study was conducted from June to September 30, 2021. The required socio-demographic and other risk factor data were collected from a total of 422 suspected TB patients using a structured questionnaire. Approximately 392 pulmonary and 30 extra-pulmonary samples were collected and examined using the Gene Xpert-MTB\/RIF assay. The statistical package for social sciences (SPSS) version 25 software was used to analyze the data.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>In this study, <jats:italic>Mycobacterium tuberculosis<\/jats:italic> was detected in 12.5% (49\/392) of pulmonary samples and 13.3% (4\/30) of extra-pulmonary samples, giving an overall TB positivity of 12.6% (53\/422). Rifampicin-resistant <jats:italic>M<\/jats:italic>. <jats:italic>tuberculosis<\/jats:italic> was detected in 3\/53 (5.7%). Male sex (AOR: 2.54; 95% CI: 1.210, 5.354), previous contact (AOR: 4.25; 95% CI: 1.790, 10.092), smoking cigarette (AOR: 4.708; 95% CI: 1.004, 22.081), being HIV-positive (AOR: 4.27; 95% CI: 1.606, 11.344), and malnutrition (AOR: 3.55; 95% CI: 1.175, 10.747) were all significantly associated with <jats:italic>M<\/jats:italic>. <jats:italic>tuberculosis<\/jats:italic> detection using the GeneXpert MTB\/RIF assay.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>The overall frequency of <jats:italic>M<\/jats:italic>. <jats:italic>tuberculosis<\/jats:italic> in this study was still significant in different risk groups, despite the proposed strategies, which aimed to reduce TB prevalence to as low as 10 per 100,000 populations by 2035. Early case detection with better diagnostic tools and public health measures are important prevention and control strategies to meet the proposed target and reduce the burden of TB in the country.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0277536","type":"journal-article","created":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T14:37:01Z","timestamp":1669300621000},"page":"e0277536","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Detection of Mycobacterium tuberculosis using Gene Xpert-MTB\/RIF assay among tuberculosis suspected patients at Mizan-Tepi university teaching hospital, southwest Ethiopia: An institution based cross-sectional study"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2700-8216","authenticated-orcid":true,"given":"Mengistu","family":"Abayneh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murtii","family":"Teressa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2022,11,23]]},"reference":[{"key":"pone.0277536.ref001","volume-title":"Global tuberculosis report 2021","author":"World Health Organization","year":"2021"},{"key":"pone.0277536.ref002","unstructured":"Parija, Subhash Chandra (2012) Textbook of Microbiology and Immunology Second Edition. 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