{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T23:58:27Z","timestamp":1771631907991,"version":"3.50.1"},"reference-count":81,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T00:00:00Z","timestamp":1686096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100009034","name":"Sea World Research and Rescue Foundation","doi-asserted-by":"publisher","award":["SWR\/1\/2015; SWR\/6\/2016"],"award-info":[{"award-number":["SWR\/1\/2015; SWR\/6\/2016"]}],"id":[{"id":"10.13039\/100009034","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Non-invasively collected faecal samples are an alternative source of DNA to tissue samples, that may be used in genetic studies of wildlife when direct sampling of animals is difficult. Although several faecal DNA extraction methods exist, their efficacy varies between species. Previous attempts to amplify mitochondrial DNA (mtDNA) markers from faeces of wild dugongs (<jats:italic>Dugong dugon<\/jats:italic>) have met with limited success and nuclear markers (microsatellites) have been unsuccessful. This study aimed to establish a tool for sampling both mtDNA and nuclear DNA (nDNA) from dugong faeces by modifying approaches used in studies of other large herbivores. First, a streamlined, cost-effective DNA extraction method that enabled the amplification of both mitochondrial and nuclear markers from large quantities of dugong faeces was developed. Faecal DNA extracted using a new \u2018High Volume- Cetyltrimethyl Ammonium Bromide- Phenol-Chloroform-Isoamyl Alcohol\u2019 (HV-CTAB-PCI) method was found to achieve comparable amplification results to extraction of DNA from dugong skin. As most prevailing practices advocate sampling from the outer surface of a stool to maximise capture of sloughed intestinal cells, this study compared amplification success of mtDNA between the outer and inner layers of faeces, but no difference in amplification was found. Assessment of the impacts of faecal age or degradation on extraction, however, demonstrated that fresher faeces with shorter duration of environmental (seawater) exposure amplified both markers better than eroded scats. Using the HV-CTAB-PCI method, nuclear markers were successfully amplified for the first time from dugong faeces. The successful amplification of single nucleotide polymorphism (SNP) markers represents a proof-of-concept showing that DNA from dugong faeces can potentially be utilised in population genetic studies. This novel DNA extraction protocol offers a new tool that will facilitate genetic studies of dugongs and other large and cryptic marine herbivores in remote locations.<\/jats:p>","DOI":"10.1371\/journal.pone.0278792","type":"journal-article","created":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T17:24:06Z","timestamp":1686158646000},"page":"e0278792","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["A new DNA extraction method (HV-CTAB-PCI) for amplification of nuclear markers from open ocean-retrieved faeces of an herbivorous marine mammal, the dugong"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9964-4038","authenticated-orcid":true,"given":"Vicky","family":"Ooi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7675-4859","authenticated-orcid":true,"given":"Lee","family":"McMichael","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret E.","family":"Hunter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aristide","family":"Takoukam Kamla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janet M.","family":"Lanyon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,6,7]]},"reference":[{"issue":"4","key":"pone.0278792.ref001","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1007\/s10592-021-01384-9","article-title":"Neutral genetic diversity as a useful tool for conservation biology","volume":"22","author":"A Garc\u00eda-Dorado","year":"2021","journal-title":"Conserv Genet"},{"issue":"48","key":"pone.0278792.ref002","doi-asserted-by":"crossref","first-page":"e2104642118","DOI":"10.1073\/pnas.2104642118","article-title":"The crucial role of genome-wide genetic variation in conservation","volume":"118","author":"M Kardos","year":"2021","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"pone.0278792.ref003","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.biocon.2005.05.002","article-title":"Genetics and extinction","volume":"126","author":"R Frankham","year":"2005","journal-title":"Biol Conserv"},{"issue":"4","key":"pone.0278792.ref004","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1111\/brv.12852","article-title":"Global genetic diversity status and trends: towards a suite of Essential Biodiversity Variables (EBVs) for genetic composition","volume":"97","author":"S Hoban","year":"2022","journal-title":"Biol Rev Camb Philos Soc"},{"issue":"1","key":"pone.0278792.ref005","first-page":"2047","article-title":"Tissue sampling methods and standards for vertebrate genomics","volume":"1","author":"PB Wong","year":"2012","journal-title":"Gigascience"},{"issue":"2","key":"pone.0278792.ref006","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1093\/jhered\/esg022","article-title":"Reliable noninvasive genotyping: fantasy or reality?","volume":"94","author":"P Fernando","year":"2003","journal-title":"J Hered"},{"issue":"8","key":"pone.0278792.ref007","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/S0169-5347(99)01637-7","article-title":"Noninvasive genetic sampling: look before you leap","volume":"14","author":"P Taberlet","year":"1999","journal-title":"Trends Ecol Evol"},{"issue":"1","key":"pone.0278792.ref008","doi-asserted-by":"crossref","first-page":"45","DOI":"10.3161\/150811012X654259","article-title":"Empirical assessment of non-invasive population genetics in bats: comparison of DNA quality from faecal and tissue samples","volume":"14","author":"ES Boston","year":"2012","journal-title":"Acta Chiropt"},{"issue":"4","key":"pone.0278792.ref009","doi-asserted-by":"crossref","first-page":"195","DOI":"10.2981\/wlb.00096","article-title":"A simple and effective method for obtaining mammal DNA from faeces","volume":"21","author":"A Ram\u00f3n\u2010Laca","year":"2015","journal-title":"Wildlife 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Zhang","year":"2019","journal-title":"BMC Biotechnol"},{"issue":"6","key":"pone.0278792.ref013","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1177\/24726303211024562","article-title":"Coprocytobiology: A technical review of cytological colorectal cancer screening in fecal samples","volume":"26","author":"L Ryan","year":"2021","journal-title":"SLAS Technol"},{"issue":"7","key":"pone.0278792.ref014","doi-asserted-by":"crossref","first-page":"2006","DOI":"10.1158\/1055-9965.EPI-08-1145","article-title":"Isolation of stool-derived mucus provides a high yield of colonocytes suitable for early detection of colorectal carcinoma","volume":"18","author":"V White","year":"2009","journal-title":"Cancer Epidemiol Biomarkers Prev"},{"issue":"8","key":"pone.0278792.ref015","doi-asserted-by":"crossref","first-page":"1430","DOI":"10.2193\/2008-492","article-title":"An improved field method to obtain DNA for individual identification from wolf scat","volume":"73","author":"LY Rutledge","year":"2009","journal-title":"J Wildl Manage"},{"issue":"3","key":"pone.0278792.ref016","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1093\/czoolo\/60.3.351","article-title":"Evaluating preservation medium for the storage of DNA in African lion Panthera leo faecal samples","volume":"60","author":"T Tende","year":"2014","journal-title":"Curr Zool"},{"issue":"4","key":"pone.0278792.ref017","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1046\/j.1471-8278.2001.00098.x","article-title":"Reliable microsatellite genotyping of dolphin DNA from faeces","volume":"1","author":"KM Parsons","year":"2001","journal-title":"Mol Ecol Notes"},{"issue":"6","key":"pone.0278792.ref018","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1111\/j.1471-8286.2007.01858.x","article-title":"Noninvasive methodology for the sampling and extraction of DNA from free\u2010ranging Atlantic spotted dolphins (Stenella frontalis).","volume":"7","author":"ML Green","year":"2007","journal-title":"Mol Ecol Notes"},{"key":"pone.0278792.ref019","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1007\/s10592-007-9361-8","article-title":"A new 2CTAB\/PCI method improves DNA amplification success from faeces of Mediterranean (Barbary macaques) and tropical (lowland gorillas) primates","volume":"9","author":"D Vallet","year":"2008","journal-title":"Conserv Genet"},{"key":"pone.0278792.ref020","article-title":"Dugong dugon (amended version of 2015 assessment).","author":"H Marsh","journal-title":"The IUCN Red List of Threatened Species 2019:e"},{"issue":"9","key":"pone.0278792.ref021","doi-asserted-by":"crossref","first-page":"e0219350","DOI":"10.1371\/journal.pone.0219350","article-title":"Phylogeography of the dugong (Dugong dugon) based on historical samples identifies vulnerable Indian Ocean populations","volume":"14","author":"S Plon","year":"2019","journal-title":"PLoS One"},{"issue":"1","key":"pone.0278792.ref022","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1071\/WR9780001","article-title":"Behaviour and ecology of the Dugong, Dugong dugon (Sirenia): observations in Shoalwater and Cleveland Bays, Queensland","volume":"5","author":"PK Anderson","year":"1978","journal-title":"Aust Wildl Res"},{"key":"pone.0278792.ref023","author":"H Marsh","year":"2011"},{"issue":"1","key":"pone.0278792.ref024","doi-asserted-by":"crossref","first-page":"6151","DOI":"10.1038\/s41598-022-09992-2","article-title":"Trajectory to local extinction of an isolated dugong population near Okinawa Island, Japan.","volume":"12","author":"H Kayanne","year":"2022","journal-title":"Sci Rep"},{"key":"pone.0278792.ref025","unstructured":"McDonald BJ. Population genetics of dugongs around Australia: implications of gene flow and migration [PhD Thesis]: James Cook University; 2005."},{"key":"pone.0278792.ref026","unstructured":"Tikel D. Using a genetic approach to optimise dugong (Dugong dugon) conservation management [PhD Thesis]: James Cook University; 1997. Available from: http:\/\/researchonline.jcu.edu.au\/28125\/."},{"issue":"1","key":"pone.0278792.ref027","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/0169-5347(89)90006-2","article-title":"Animal mitochondrial DNA as a genetic marker in population and evolutionary biology","volume":"4","author":"RG Harrison","year":"1989","journal-title":"Trends Ecol Evol"},{"key":"pone.0278792.ref028","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1007\/s10592-014-0624-x","article-title":"Fine scale population structure of dugongs (Dugong dugon) implies low gene flow along the southern Queensland coastline","volume":"15","author":"JM Seddon","year":"2014","journal-title":"Conserv Genet"},{"key":"pone.0278792.ref029","article-title":"Cryptic marine barriers to gene flow in a vulnerable coastal species, the dugong (Dugong dugon).","author":"AM McGowan","year":"2023","journal-title":"Mar Mammal Sci."},{"issue":"2","key":"pone.0278792.ref030","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/S0378-1119(99)00219-X","article-title":"The essence of SNPs","volume":"234","author":"AJ Brookes","year":"1999","journal-title":"Gene"},{"issue":"4","key":"pone.0278792.ref031","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.tree.2004.01.009","article-title":"SNPs in ecology, evolution and conservation","volume":"19","author":"PA Morin","year":"2004","journal-title":"Trends Ecol Evol"},{"issue":"3","key":"pone.0278792.ref032","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.fsigen.2011.07.010","article-title":"A new SNP assay for identification of highly degraded human DNA","volume":"6","author":"A Freire-Aradas","year":"2012","journal-title":"Forensic Sci Int Genet"},{"issue":"1","key":"pone.0278792.ref033","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.forsciint.2005.11.024","article-title":"Nuclear and mitochondrial DNA quantification of various forensic materials","volume":"164","author":"H Andr\u00e9asson","year":"2006","journal-title":"Forensic Sci Int"},{"key":"pone.0278792.ref034","unstructured":"Takoukam Kamla A. Genetic diversity, diet, and habitat quality of the African manatee (Trichechus senegalensis) in the downstream of the Sanaga River Watershed, Cameroon [PhD Thesis]: University of Florida; 2019."},{"key":"pone.0278792.ref035","doi-asserted-by":"crossref","first-page":"39","DOI":"10.5597\/lajam00132","article-title":"Diet of the manatees (Trichechus manatus manatus) in Chetumal Bay, Mexico.","volume":"7","author":"DN Castelblanco-Mart\u00ednez","year":"2009","journal-title":"Lat Am J Aquat Mamm"},{"key":"pone.0278792.ref036","unstructured":"Lanyon J. The nutritional ecology of the dugong in tropical North Queensland [PhD Thesis]: James Cook University; 1991."},{"issue":"2","key":"pone.0278792.ref037","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1111\/j.1469-7998.2006.00135.x","article-title":"Mechanical disruption of seagrass in the digestive tract of the dugong","volume":"270","author":"JM Lanyon","year":"2006","journal-title":"J Zool"},{"issue":"1A","key":"pone.0278792.ref038","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/0300-9629(77)90432-7","article-title":"The role of the midgut caecum and large intestine in the digestion of sea grasses by the dugong (Mammalia: Sirenia).","volume":"56","author":"RM Murray","year":"1977","journal-title":"Comp Biochem Physiol A Physiol"},{"issue":"1","key":"pone.0278792.ref039","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/j.1365-2907.1981.tb00243.x","article-title":"Foods and feeding habits of wild and captive Sirenia","volume":"11","author":"RC Best","year":"1981","journal-title":"Mamm Rev"},{"issue":"1","key":"pone.0278792.ref040","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1578\/AM.36.1.2010.46","article-title":"Physiological response of wild dugongs (Dugong dugon) to out-of-water sampling for health assessment","volume":"36","author":"JM Lanyon","year":"2010","journal-title":"Aquat Mamm"},{"issue":"1","key":"pone.0278792.ref041","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1071\/AM02051","article-title":"Establishing a mark-recapture program for dugongs in Moreton Bay, south-east Queensland","volume":"24","author":"JM Lanyon","year":"2002","journal-title":"Aust Mammal."},{"key":"pone.0278792.ref042","article-title":"QIAamp DNA Extraction Protocol.","author":"V Ooi","year":"2023","journal-title":"protocols.io"},{"key":"pone.0278792.ref043","article-title":"HV-CTAB-PCI DNA Extraction Protocol.","author":"V Ooi","year":"2023","journal-title":"protocols.io"},{"key":"pone.0278792.ref044","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/s12686-020-01187-z","article-title":"Using DNA to distinguish between faeces of Dugong dugon and Chelonia mydas: non-invasive sampling for IUCN-listed marine megafauna","volume":"13","author":"S Tol","year":"2021","journal-title":"Conserv Genet Resour"},{"issue":"3","key":"pone.0278792.ref045","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1111\/j.1471-8286.2007.02041.x","article-title":"A PCR assay for gender assignment in dugong (Dugong dugon) and West Indian manatee (Trichechus manatus).","volume":"8","author":"M McHale","year":"2008","journal-title":"Mol Ecol Resour"},{"issue":"6","key":"pone.0278792.ref046","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/gkp045","article-title":"Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data","volume":"37","author":"JM Ruijter","year":"2009","journal-title":"Nucleic Acids Res"},{"key":"pone.0278792.ref047","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.bdq.2017.04.001","article-title":"Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR).","volume":"12","author":"A Forootan","year":"2017","journal-title":"Biomol Detect Quantif"},{"issue":"7","key":"pone.0278792.ref048","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: molecular evolutionary genetics analysis Version 11","volume":"38","author":"K Tamura","year":"2021","journal-title":"Mol Biol Evol"},{"key":"pone.0278792.ref049","author":"R Core Team","year":"2022"},{"key":"pone.0278792.ref050","author":"H. Wickham","year":"2017"},{"key":"pone.0278792.ref051","article-title":"hrbrthemes: Additional themes, theme components and utilities for \u2019ggplot2\u2019","author":"B. Rudis","year":"2020","journal-title":"R package version 0.8.0 [Internet]"},{"key":"pone.0278792.ref052","article-title":"Rmisc: Ryan miscellaneous. R package version 1.5.1 [Internet].","author":"R. Hope","year":"2022"},{"key":"pone.0278792.ref053","article-title":"ggpmisc: Miscellaneous extensions to \u2019ggplot2\u2019. R package version 0.4.7 [Internet].","author":"P. Aphalo","year":"2022"},{"key":"pone.0278792.ref054","article-title":"ggsignif: R Package for Displaying Significance Brackets for \u2019ggplot2\u2019","author":"C Ahlmann-Eltze","year":"2021","journal-title":"PsyArxiv"},{"key":"pone.0278792.ref055","article-title":"pubr: \u2019ggplot2\u2019 based publication ready plots. R package version 0.4.0 [Internet]","author":"A. Kassambara","year":"2020"},{"key":"pone.0278792.ref056","article-title":"R Core Team. nlme: Linear and nonlinear mixed effects models. R package version 3 [Internet].","author":"J Pinheiro","year":"2022"},{"key":"pone.0278792.ref057","doi-asserted-by":"crossref","DOI":"10.1007\/978-0-387-21706-2","volume-title":"Modern applied statistics with S","author":"W Venables","year":"2002","edition":"4"},{"key":"pone.0278792.ref058","article-title":"brglm2: Bias reduction in generalized linear models","author":"I. Kosmidis","year":"2021","journal-title":"R package version 0.8.2 [Internet]."},{"key":"pone.0278792.ref059","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.bdq.2015.01.005","article-title":"How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments","volume":"3","author":"D Svec","year":"2015","journal-title":"Biomol Detect Quantif"},{"key":"pone.0278792.ref060","doi-asserted-by":"crossref","first-page":"e012","DOI":"10.1621\/nrs.01012","article-title":"Quantitative real-time PCR protocol for analysis of nuclear receptor signaling pathways","volume":"1","author":"AL Bookout","year":"2003","journal-title":"Nucl Recept Signal"},{"issue":"16","key":"pone.0278792.ref061","doi-asserted-by":"crossref","first-page":"3189","DOI":"10.1093\/nar\/24.16.3189","article-title":"Reliable genotyping of samples with very low DNA quantities using PCR","volume":"24","author":"P Taberlet","year":"1996","journal-title":"Nucleic Acids Res"},{"issue":"5","key":"pone.0278792.ref062","doi-asserted-by":"crossref","first-page":"e0176848","DOI":"10.1371\/journal.pone.0176848","article-title":"Low concentration DNA extraction and recovery using a silica solid phase","volume":"12","author":"C Katevatis","year":"2017","journal-title":"PLoS One"},{"issue":"4","key":"pone.0278792.ref063","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.mito.2009.03.003","article-title":"DNA extraction procedures meaningfully influence qPCR-based mtDNA copy number determination","volume":"9","author":"W Guo","year":"2009","journal-title":"Mitochondrion"},{"issue":"4","key":"pone.0278792.ref064","doi-asserted-by":"crossref","first-page":"pdb prot093450","DOI":"10.1101\/pdb.prot093450","article-title":"Isolation of high-molecular-weight DNA using organic solvents","volume":"2017","author":"MR Green","year":"2017","journal-title":"Cold Spring Harb Protoc"},{"issue":"1","key":"pone.0278792.ref065","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1111\/j.1755-0998.2009.02670.x","article-title":"Impacts of sampling location within a faeces on DNA quality in two carnivore species","volume":"10","author":"J Stenglein","year":"2010","journal-title":"Mol Ecol Resour"},{"issue":"1","key":"pone.0278792.ref066","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0046-8177(00)80198-7","article-title":"Morphometric analysis of the \u201cmucocellular layer\u201d overlying colorectal cancer and normal mucosa: relevance to exfoliation and stool screening","volume":"31","author":"DA Ahlquist","year":"2000","journal-title":"Hum Pathol"},{"issue":"5","key":"pone.0278792.ref067","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1071\/WR03096","article-title":"Effect of sample age and season of collection on the reliability of microsatellite genotyping of faecal DNA","volume":"31","author":"MP Piggott","year":"2004","journal-title":"Wildl Res"},{"issue":"6","key":"pone.0278792.ref068","doi-asserted-by":"crossref","first-page":"1776","DOI":"10.1053\/gast.1996.v110.pm8964403","article-title":"Rapid onset of apoptosis in vitro follows disruption of beta 1-integrin\/matrix interactions in human colonic crypt cells","volume":"110","author":"J Strater","year":"1996","journal-title":"Gastroenterology"},{"key":"pone.0278792.ref069","doi-asserted-by":"crossref","first-page":"a016394","DOI":"10.1101\/cshperspect.a016394","article-title":"DNA degradation and its defects","volume":"6","author":"K Kawane","year":"2014","journal-title":"Cold Spring Harb Perspect Biol"},{"issue":"5","key":"pone.0278792.ref070","doi-asserted-by":"crossref","first-page":"2736","DOI":"10.1016\/S0006-3495(99)77107-9","article-title":"Competitive binding of Mg2+, Ca2+, Na+, and K+ ions to DNA in oriented DNA fibers: experimental and Monte Carlo simulation results","volume":"77","author":"N Korolev","year":"1999","journal-title":"Biophys J"},{"issue":"7","key":"pone.0278792.ref071","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1021\/ar900197y","article-title":"A stability concept for metal ion coordination to single-stranded nucleic acids and affinities of individual sites","volume":"43","author":"RK Sigel","year":"2010","journal-title":"Acc Chem Res"},{"issue":"10","key":"pone.0278792.ref072","doi-asserted-by":"crossref","first-page":"5726","DOI":"10.1093\/nar\/gkac445","article-title":"Twisting DNA by salt","volume":"50","author":"S Cruz-Leon","year":"2022","journal-title":"Nucleic Acids Res"},{"issue":"10","key":"pone.0278792.ref073","doi-asserted-by":"crossref","first-page":"e110753","DOI":"10.1371\/journal.pone.0110753","article-title":"Post mortem DNA degradation of human tissue experimentally mummified in salt","volume":"9","author":"N Shved","year":"2014","journal-title":"PLoS 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Matsushita","year":"2005","journal-title":"Gastroenterology"},{"key":"pone.0278792.ref077","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1186\/1471-2164-14-439","article-title":"Fish scales and SNP chips: SNP genotyping and allele frequency estimation in individual and pooled DNA from historical samples of Atlantic salmon (Salmo salar).","volume":"14","author":"SE Johnston","year":"2013","journal-title":"BMC Genomics"},{"issue":"6","key":"pone.0278792.ref078","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1111\/1755-0998.13208","article-title":"More affordable and effective noninvasive single nucleotide polymorphism genotyping using high-throughput amplicon sequencing","volume":"20","author":"CE Eriksson","year":"2020","journal-title":"Mol Ecol Resour"},{"issue":"10","key":"pone.0278792.ref079","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1046\/j.1365-294x.1998.00449.x","article-title":"Empirical evaluation of preservation methods for faecal 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