{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T06:06:18Z","timestamp":1792649178179,"version":"4.5.0"},"reference-count":52,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2022,12,15]],"date-time":"2022-12-15T00:00:00Z","timestamp":1671062400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Macrophages play a crucial role in inflammation, a defense mechanism of the innate immune system. Metabolic function powered by glucose transporter isoform 1 (Glut1) is necessary for macrophage activity during inflammation. The present study investigated the roles of cystathionine-\u03b3-lyase (CSE) and its byproduct, hydrogen sulfide (H\n                    <jats:sub>2<\/jats:sub>\n                    S), in macrophage glucose metabolism to explore the mechanism by which H\n                    <jats:sub>2<\/jats:sub>\n                    S acts as an inflammatory regulator in lipopolysaccharide- (LPS) induced macrophages. Our results demonstrated that LPS-treated macrophages increased Glut1 expression. LPS-induced Glut1 expression is regulated via nuclear factor (NF)-\u03baB activation and is associated with phosphatidylinositol-3-kinase PI3k activation. Small interfering (si) RNA-mediated silencing of CSE decreased the LPS-induced NF-\u03baB activation and Glut1 expression, suggesting a role for H\n                    <jats:sub>2<\/jats:sub>\n                    S in metabolic function in macrophages during pro-inflammatory response. Confoundingly, treatment with GYY4137, an H\n                    <jats:sub>2<\/jats:sub>\n                    S-donor molecule, also displayed inhibitory effects upon LPS-induced NF-\u03baB activation and Glut1 expression. Moreover, GYY4137 treatment increased Akt activation, suggesting a role in promoting resolution of inflammation. Our study provides evidence that the source of H\n                    <jats:sub>2<\/jats:sub>\n                    S, either endogenous (via CSE) or exogenous (via GYY4137), supports or inhibits the LPS-induced NF-\u03baB activity and Glut1 expression, respectively. Therefore, H\n                    <jats:sub>2<\/jats:sub>\n                    S may influence metabolic programming in immune cells to alter glucose substrate availability that impacts the immune response.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0278910","type":"journal-article","created":{"date-parts":[[2022,12,15]],"date-time":"2022-12-15T13:37:05Z","timestamp":1671111425000},"page":"e0278910","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":21,"title":["Cystathionine \u03b3-lyase and hydrogen sulfide modulates glucose transporter Glut1 expression via NF-\u03baB and PI3k\/Akt in macrophages during inflammation"],"prefix":"10.1371","volume":"17","author":[{"given":"Alex","family":"Cornwell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha","family":"Fedotova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Cowan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5692-091X","authenticated-orcid":true,"given":"Alireza","family":"Badiei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,12,15]]},"reference":[{"key":"pone.0278910.ref001","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1111\/bph.14772","article-title":"The role of the gasotransmitter hydrogen sulfide in pathological calcification","volume":"177","author":"M Castelblanco","year":"2020","journal-title":"Br J Pharmacol"},{"key":"pone.0278910.ref002","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1111\/bph.14579","article-title":"Signalling by hydrogen sulfide and polysulfides via protein S-sulfuration","volume":"177","author":"H. 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