{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T20:21:01Z","timestamp":1772742061419,"version":"3.50.1"},"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2022,12,20]],"date-time":"2022-12-20T00:00:00Z","timestamp":1671494400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Polycystic kidney disease (PKD), also known as autosomal dominant polycystic kidney disease (ADPKD) is a genetically heterogeneous condition characterized by cysts in renal parenchyma. It is the most prevalent inherited disease of domestic cats. MicroRNAs (miRNAs or ncRNA) are short, noncoding, single-stranded RNAs that may induce PKD cytogenesis by affecting numerous targets genes as well as by directly regulating PKD gene expression. We compared the relative expression profile of miR-20a, -192, -365, -15b-5p, and -16-5p from plasma and serum samples of nine domestic cats with <jats:italic>PKD1<\/jats:italic> mutation, detected by polymerase chain reaction (PCR), and a control group (n = 10). Blood samples from cats with <jats:italic>PKD1<\/jats:italic> mutation provide similar concentrations of microRNAs either from plasma or serum. Serum miR-20a is upregulated in PKD group with p &lt; 0.005; Roc curve analysis showed an AUC of 90,1% with a cut-off value sensitivity of 77.8% and specificity of 100%. This data provides important information regarding renal miRNA expression in peripheral blood sampling.<\/jats:p>","DOI":"10.1371\/journal.pone.0279337","type":"journal-article","created":{"date-parts":[[2022,12,20]],"date-time":"2022-12-20T18:34:55Z","timestamp":1671561295000},"page":"e0279337","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["miR-20a is upregulated in serum from domestic feline with PKD1 mutation"],"prefix":"10.1371","volume":"17","author":[{"given":"Marcela Correa","family":"Scalon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine Souza","family":"Martins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel Ginani","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franciele","family":"Schlemmer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"Titze de Almeida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9112-1718","authenticated-orcid":true,"given":"Giane Regina","family":"Paludo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2022,12,20]]},"reference":[{"issue":"10","key":"pone.0279337.ref001","doi-asserted-by":"crossref","first-page":"2548","DOI":"10.1097\/01.ASN.0000141776.38527.BB","article-title":"Feline polycystic kidney disease mutation identified in PKD1","volume":"15","author":"LA Lyons","year":"2004","journal-title":"J Am Soc Nephrol"},{"issue":"3","key":"pone.0279337.ref002","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1177\/104063871002200314","article-title":"Molecular detection of autosomal-dominant feline polycystic kidney disease by multiplex amplification refractory mutation system polymerase chain reaction","volume":"22","author":"YJ Lee","year":"2010","journal-title":"J Vet Diagn Invest"},{"issue":"4","key":"pone.0279337.ref003","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1053\/j.tcam.2010.09.002","article-title":"Feline genetics: clinical applications and genetic testing","volume":"25","author":"LA Lyons","year":"2010","journal-title":"Top Companion Anim Med"},{"issue":"4","key":"pone.0279337.ref004","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1177\/1040638714536561","article-title":"Touchdown polymerase chain reaction detection of polycystic kidney disease and laboratory findings in different cat populations","volume":"26","author":"MC Scalon","year":"2014","journal-title":"J Vet Diagn Invest"},{"issue":"1","key":"pone.0279337.ref005","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/s00467-013-2706-2","article-title":"ARPKD and early manifestations of ADPKD: the original polycystic kidney disease and phenocopies","volume":"30","author":"C. 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