{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T05:58:44Z","timestamp":1778392724178,"version":"3.51.4"},"reference-count":30,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2023,2,17]],"date-time":"2023-02-17T00:00:00Z","timestamp":1676592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000289","name":"Cancer Research UK","doi-asserted-by":"publisher","award":["A28345"],"award-info":[{"award-number":["A28345"]}],"id":[{"id":"10.13039\/501100000289","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Discrimination of brain cancer <jats:italic>versus<\/jats:italic> non-cancer patients using serum-based attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy diagnostics was first developed by Hands <jats:italic>et al<\/jats:italic> with a reported sensitivity of 92.8% and specificity of 91.5%. Cameron <jats:italic>et al<\/jats:italic>. then went on to stratifying between specific brain tumour types: glioblastoma multiforme (GBM) vs. primary cerebral lymphoma with a sensitivity of 90.1% and specificity of 86.3%. Expanding on these studies, 30 GBM, 30 lymphoma and 30 non-cancer patients were selected to investigate the influence on test performance by focusing on specific molecular weight regions of the patient serum. Membrane filters with molecular weight cut offs of 100 kDa, 50 kDa, 30 kDa, 10 kDa and 3 kDa were purchased in order to remove the most abundant high molecular weight components. Three groups were classified using both partial least squares-discriminate analysis (PLS-DA) and random forest (RF) machine learning algorithms; GBM <jats:italic>versus<\/jats:italic> non-cancer, lymphoma <jats:italic>versus<\/jats:italic> non-cancer and GBM <jats:italic>versus<\/jats:italic> lymphoma. For all groups, once the serum was filtered the sensitivity, specificity and overall balanced accuracies decreased. This illustrates that the high molecular weight components are required for discrimination between cancer and non-cancer as well as between tumour types. From a clinical application point of view, this is preferable as less sample preparation is required.<\/jats:p>","DOI":"10.1371\/journal.pone.0279669","type":"journal-article","created":{"date-parts":[[2023,2,17]],"date-time":"2023-02-17T18:39:23Z","timestamp":1676659163000},"page":"e0279669","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":13,"title":["Investigating centrifugal filtration of serum-based FTIR spectroscopy for the stratification of brain tumours"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8754-5094","authenticated-orcid":true,"given":"Ashton G.","family":"Theakstone","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul M.","family":"Brennan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael D.","family":"Jenkinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Royston","family":"Goodacre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2362-8581","authenticated-orcid":true,"given":"Matthew J.","family":"Baker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,2,17]]},"reference":[{"issue":"8","key":"pone.0279669.ref001","doi-asserted-by":"crossref","first-page":"e029686","DOI":"10.1136\/bmjopen-2019-029686","article-title":"The usefulness of symptoms alone or combined for general practitioners in considering the diagnosis of a brain tumour: a case-control study using the clinical practice research database (CPRD) (2000\u20132014).","volume":"9","author":"M Ozawa","year":"2019","journal-title":"BMJ Open"},{"issue":"542","key":"pone.0279669.ref002","first-page":"695","article-title":"Clinical features of primary brain tumours: a case-control study using electronic primary care records","volume":"57","author":"W Hamilton","year":"2007","journal-title":"Br J Gen Pract"},{"issue":"3","key":"pone.0279669.ref003","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1136\/jnnp.2005.073221","article-title":"Headache and migraine in primary care: consultation, prescription, and referral rates in a large population","volume":"77","author":"R. 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