{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T10:54:51Z","timestamp":1789469691762,"version":"build-2803163510"},"reference-count":38,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2023,2,10]],"date-time":"2023-02-10T00:00:00Z","timestamp":1675987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Long Term Research Grant"},{"DOI":"10.13039\/501100003093","name":"Ministry of Higher Education Malaysia","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"crossref"}]},{"name":"QIAGEN"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Diagnosis and treatment of tuberculosis infection (TBI) are the core elements of tuberculosis elimination. Interferon gamma release assays have advantages over the tuberculin skin test, although their implementation in low-resource settings is challenging. The performance of a novel digital lateral flow assay QIAreach<jats:sup>\u00ae<\/jats:sup> QuantiFERON<jats:sup>\u00ae<\/jats:sup>-TB (QIAreach QFT) against the QuantiFERON<jats:sup>\u00ae<\/jats:sup>-TB Gold Plus (QFT-Plus) assay was evaluated in an intermediate incidence setting (Malaysia) according to the manufacturer\u2019s instructions. Individuals aged 4\u201382 years, who were candidates for TB infection screening for contact investigation were prospectively recruited. On 196 samples, the QIAreach-QFT showed a positive percent agreement (sensitivity) was 96.5% (CI 87.9\u201399.6%), a negative percent agreement (specificity) 94.2% (CI 88.4% to 97.6%) and an overall percentage of agreement was 94.9% (95% CI 90.6\u201397.6%) with a Cohen\u2019s \u03ba of 0,88. Out of 196, 5.6% (11\/196) samples gave an error result on QIAreach-QFT and 4.1% (8\/196) samples gave indeterminate result on QFT-plus. The TTR for QIAreach QFT positive samples varied from 210\u20131200 seconds (20 min) and significantly correlated with IFN-\u03b3 level of QFT-Plus. QIAreach QFT could be considered an accurate and reliable point-of-need test to diagnose TB infection helping to achieve the WHO End TB programme goals even in decentralised settings where laboratory expertise and infrastructure may be limited.<\/jats:p>","DOI":"10.1371\/journal.pone.0279882","type":"journal-article","created":{"date-parts":[[2023,2,10]],"date-time":"2023-02-10T19:09:04Z","timestamp":1676056144000},"page":"e0279882","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["First evaluation of the performance of portable IGRA, QIAreach\u00ae QuantiFERON\u00ae-TB in intermediate TB incidence setting"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0930-4671","authenticated-orcid":true,"given":"Zirwatul Adilah","family":"Aziz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noorliza Mohamad","family":"Noordin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan Mazlina","family":"Wan Mohd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohd Amin","family":"Kasim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,2,10]]},"reference":[{"key":"pone.0279882.ref001","article-title":"Global Tuberculosis Report 2021","author":"World Health Organization (WHO)","year":"2021"},{"key":"pone.0279882.ref002","article-title":"WHO operational handbook on tuberculosis (Module 1 \u2013Prevention): Tuberculosis preventive treatment","volume":"140","author":"World Health Organization (WHO)","year":"2020"},{"key":"pone.0279882.ref003","unstructured":"WHO. 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