{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T08:53:56Z","timestamp":1765961636401,"version":"3.40.5"},"reference-count":48,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2023,2,17]],"date-time":"2023-02-17T00:00:00Z","timestamp":1676592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science Center, Poland","award":["2014\/15\/B\/NZ5\/O3572"],"award-info":[{"award-number":["2014\/15\/B\/NZ5\/O3572"]}]},{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education","doi-asserted-by":"crossref","award":["010\/RID\/2018\/19"],"award-info":[{"award-number":["010\/RID\/2018\/19"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Disturbances in uterine contractility often lead to the origin, development and maintenance of endometritis and metritis, which are a very common and serious pathologies in domestic animals. Here we aimed to investigate the role of \u03b11A-, \u03b11B- and \u03b11D-adrenoreceptors (ARs) in noradrenaline (NA)-induced contractility of inflammatory-changed porcine uterus.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>On Day 3 of the estrous cycle, either <jats:italic>Escherichia coli<\/jats:italic> (<jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic>) suspension (<jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> group) or saline (SAL group) was injected into uterine horns, or only laparotomy was performed (CON group). Eight days later, infected gilts developed severe acute endometritis.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>Compared to the period before NA application, NA reduced the contractile amplitude and frequency in myometrium (MYO) and endometrium (ENDO)\/MYO strips from the CON, SAL and <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> groups. In the last group, the amplitude in MYO and the frequency in ENDO\/MYO were lowered versus other groups. After using \u03b11A-ARs antagonist with NA, a greater decrease or occurrence of a drop in the amplitude and frequency in all groups (ENDO\/MYO) were found compared to this neurotransmitter action alone. Such results were noted for NA action on the frequency after \u03b11B-ARs blocking in the CON (both kinds of strips) and SAL (ENDO\/MYO) groups. In response to \u03b11D-ARs antagonist with NA, a greater decrease or occurrence of a drop in the amplitude was noted in the CON (both kinds of strips) and SAL and <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> (MYO) groups. Use of these factors caused the similar changes in the frequency in CON and <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> (MYO) and SAL (ENDO\/MYO) groups. In response to NA, \u03b11A,B,D-ARs antagonist led to a greater reduction or appearance of a drop in the amplitude in the CON and SAL (ENDO\/MYO) and <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> (both kinds of strips) as well as in the frequency in the CON and SAL (ENDO\/MYO) and <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> (MYO) groups.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusions<\/jats:title>\n<jats:p>In conclusion, activation of \u03b11A- and \u03b11D-ARs by NA promotes the contractile amplitude and frequency in the inflamed pig uterus; pharmacological modulation of these receptors can be utilized to enhance systolic activity of myometrium.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0280152","type":"journal-article","created":{"date-parts":[[2023,2,17]],"date-time":"2023-02-17T18:35:56Z","timestamp":1676658956000},"page":"e0280152","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of blocking of alpha1-adrenoreceptor isoforms on the noradrenaline-induced changes in contractility of inflamed pig uterus"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6508-2965","authenticated-orcid":true,"given":"Barbara","family":"Jana","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaros\u0142aw","family":"Ca\u0142ka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,2,17]]},"reference":[{"key":"pone.0280152.ref001","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.ando.2020.03.012","article-title":"Adrenergic receptors and cardiovascular effects of catecholamines","volume":"82","author":"J. 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