{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T16:33:36Z","timestamp":1792427616254,"version":"4.5.0"},"reference-count":22,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2023,1,20]],"date-time":"2023-01-20T00:00:00Z","timestamp":1674172800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"name":"MIDRP","award":["D0542_17_AF"],"award-info":[{"award-number":["D0542_17_AF"]}]},{"DOI":"10.13039\/100009896","name":"U.S. Navy","doi-asserted-by":"publisher","award":["6000.RAD1.DA3.A0308"],"award-info":[{"award-number":["6000.RAD1.DA3.A0308"]}],"id":[{"id":"10.13039\/100009896","id-type":"DOI","asserted-by":"publisher"}]},{"name":"AFHSD-GEIS"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p><jats:italic>Campylobacter jejuni<\/jats:italic> is a major cause of bacterial diarrhea worldwide and associated with numerous sequela, including Guillain-Barr\u00e9 Syndrome, inflammatory bowel disease, reactive arthritis, and irritable bowel syndrome. <jats:italic>C<\/jats:italic>. <jats:italic>jejuni<\/jats:italic> is unusual for an intestinal pathogen in its ability to coat its surface with a polysaccharide capsule (CPS). The genes responsible for the biosynthesis of the phase variable CPS is located in the hypervariable region of <jats:italic>C<\/jats:italic>. <jats:italic>jejuni<\/jats:italic> genome which has been used to develop multiplex PCR to classify CPS types based on the Penner serotypes. However, there still are non-typable CPS <jats:italic>C<\/jats:italic>. <jats:italic>jejuni<\/jats:italic> by the current multiplex PCR scheme. The application of the next generation sequencing and whole genome analysis software were used for the identification of novel capsule biosynthesis of <jats:italic>C<\/jats:italic>. <jats:italic>jejuni<\/jats:italic> isolates. Unique PCR primers were designed to identify these new capsule biosynthesis loci. The designed primers sets were combined in a new multiplex mix called epsilon. The unique sequences provide an additional information of the biosynthesis loci responsible for some of the common CPS sugars\/residues such as heptose, deoxtyheptose and MeOPN among <jats:italic>C<\/jats:italic>. <jats:italic>jejuni<\/jats:italic> in this new group of CPS multiplex assay. This new primer complements the current <jats:italic>C<\/jats:italic>. <jats:italic>jejuni<\/jats:italic> multiplex capsule typing system and will help in identifying previously untypeable capsule locus of <jats:italic>C<\/jats:italic>. <jats:italic>jejuni<\/jats:italic> isolates.<\/jats:p>","DOI":"10.1371\/journal.pone.0280583","type":"journal-article","created":{"date-parts":[[2023,1,20]],"date-time":"2023-01-20T18:38:44Z","timestamp":1674239924000},"page":"e0280583","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Description of novel capsule biosynthesis loci of Campylobacter jejuni clinical isolates from South and South-East Asia"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3618-7519","authenticated-orcid":true,"given":"Nuanpan","family":"Khemnu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oralak","family":"Serichantalergs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sirigade","family":"Ruekit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2993-9794","authenticated-orcid":true,"given":"Paphavee","family":"Lertsethtakarn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Poly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett E.","family":"Swierczewski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M.","family":"Crawford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,1,20]]},"reference":[{"issue":"12","key":"pone.0280583.ref001","doi-asserted-by":"crossref","first-page":"6292","DOI":"10.3390\/ijerph10126292","article-title":"Foodborne Campylobacter: infections, metabolism, pathogenesis and reservoirs","volume":"10","author":"SV Epps","year":"2013","journal-title":"International journal of environmental research and public health"},{"key":"pone.0280583.ref002","first-page":"190","volume-title":"Manual of Clinical Microbiology","author":"D. 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Guerry","year":"2007","journal-title":"Trends Microbiol"},{"issue":"21","key":"pone.0280583.ref011","doi-asserted-by":"crossref","first-page":"4407","DOI":"10.1093\/nar\/23.21.4407","article-title":"AFLP: a new technique for DNA fingerprinting","volume":"23","author":"P Vos","year":"1995","journal-title":"Nucleic acids research"},{"issue":"12","key":"pone.0280583.ref012","doi-asserted-by":"crossref","first-page":"e0144349","DOI":"10.1371\/journal.pone.0144349","article-title":"Updated Campylobacter jejuni Capsule PCR Multiplex Typing System and Its Application to Clinical Isolates from South and Southeast Asia","volume":"10","author":"F Poly","year":"2015","journal-title":"PloS one"},{"issue":"5","key":"pone.0280583.ref013","doi-asserted-by":"crossref","first-page":"1750","DOI":"10.1128\/JCM.02348-10","article-title":"Discrimination of major capsular types of Campylobacter jejuni by multiplex PCR","volume":"49","author":"F Poly","year":"2011","journal-title":"Journal of clinical microbiology"},{"issue":"3","key":"pone.0280583.ref014","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1046\/j.1365-2958.2001.02431.x","article-title":"A phase-variable capsule is involved in virulence of Campylobacter jejuni 81\u2013176","volume":"40","author":"DJ Bacon","year":"2001","journal-title":"Molecular microbiology"},{"issue":"6","key":"pone.0280583.ref015","doi-asserted-by":"crossref","first-page":"e67375","DOI":"10.1371\/journal.pone.0067375","article-title":"Global Distribution of Campylobacter jejuni Penner Serotypes: A Systematic Review","volume":"8","author":"BL Pike","year":"2013","journal-title":"PloS one"},{"issue":"12","key":"pone.0280583.ref016","doi-asserted-by":"crossref","first-page":"5549","DOI":"10.1128\/JCM.42.12.5549-5557.2004","article-title":"Differentiation of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis by a multiplex PCR developed from the nucleotide sequence of the lipid A gene lpxA","volume":"42","author":"JD Klena","year":"2004","journal-title":"Journal of clinical microbiology"},{"issue":"4","key":"pone.0280583.ref017","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1073\/pnas.1017351108","article-title":"High-quality draft assemblies of mammalian genomes from massively parallel sequence data","volume":"108","author":"S Gnerre","year":"2011","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"2","key":"pone.0280583.ref018","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1101\/gr.097261.109","article-title":"De novo assembly of human genomes with massively parallel short read sequencing","volume":"20","author":"R Li","year":"2010","journal-title":"Genome research"},{"issue":"5","key":"pone.0280583.ref019","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1101\/gr.074492.107","article-title":"Velvet: algorithms for de novo short read assembly using de Bruijn graphs","volume":"18","author":"DR Zerbino","year":"2008","journal-title":"Genome research"},{"issue":"1","key":"pone.0280583.ref020","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1111\/j.1365-2958.2004.04374.x","article-title":"Analysis of Campylobacter jejuni capsular loci reveals multiple mechanisms for the generation of structural diversity and the ability to form complex heptoses","volume":"55","author":"AV Karlyshev","year":"2005","journal-title":"Mol Microbiol"},{"issue":"10","key":"pone.0280583.ref021","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1093\/bioinformatics\/16.10.944","article-title":"Artemis: sequence visualization and annotation","volume":"16","author":"K Rutherford","year":"2000","journal-title":"Bioinformatics"},{"issue":"7","key":"pone.0280583.ref022","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets","volume":"33","author":"S Kumar","year":"2016","journal-title":"Molecular Biology and Evolution"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0280583","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,20]],"date-time":"2023-01-20T18:39:12Z","timestamp":1674239952000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0280583"}},"subtitle":[],"editor":[{"given":"Joel","family":"Mossong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,1,20]]},"references-count":22,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,1,20]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0280583","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,20]]}}}