{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T08:43:47Z","timestamp":1768639427483,"version":"3.49.0"},"reference-count":28,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2023,7,6]],"date-time":"2023-07-06T00:00:00Z","timestamp":1688601600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["305741\/2018-7"],"award-info":[{"award-number":["305741\/2018-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Ionized water has been reported to contribute to the tissue repair process and wound healing. Water purifiers can generate ionized water by means of activated charcoal with silver and minerals, the main purpose of which are to reduce microbiological and physicochemical contaminants. Moreover, when water is subjected to a magnetic field an organization of water molecules occurs due to the presence of mineral salts. The resulting water is thus more alkaline, which has been shown to be non-toxic to mice and can actually prolong survival. Cutaneous leishmaniasis is a neglected tropical disease, caused by obligate uni- and intracellular protozoa belonging to the genus <jats:italic>Leishmania<\/jats:italic>, that can manifest in the form of skin lesions. Thus, the objective of this study was to compare the evolution of disease in <jats:italic>L<\/jats:italic>. <jats:italic>amazonensis<\/jats:italic>-infected BALB\/c mice that received tap water (TW) or ionized alkaline water (IAW). As a control, additional groups of mice receiving TW or IAW were also treated with the antileishmanial miltefosine. All mouse groups received either TW or IAW as drinking water 30 days prior to infection and the groups continued to receive the respective drinking water for 4 weeks, after which the blood and plasma were collected. Biochemical assays of aspartate aminotransferase, alanine aminotransferase, gamma-glutamyl transferase, creatinine, urea, glucose, triglycerides, and cholesterol were performed, in addition to hematology tests. There was a significant decrease in the volume of the lesion for groups that received IAW, in which the ingestion of ionized alkaline water favored the non-evolution of the lesion in the footpads of the animals. The results of the blood count and leukogram tests were within the normal values for BALB\/c mice demonstrating that ionized water has no toxic effects on blood factors.<\/jats:p>","DOI":"10.1371\/journal.pone.0280695","type":"journal-article","created":{"date-parts":[[2023,7,6]],"date-time":"2023-07-06T17:34:11Z","timestamp":1688664851000},"page":"e0280695","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Ionized alkaline water reduces injury in BALB\/c mice infected with Leishmania amazonensis"],"prefix":"10.1371","volume":"18","author":[{"given":"Karina Miyuki","family":"Retamiro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karine Campos","family":"Nunes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aline 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Available from: https:\/\/www.blog.purific.com.br\/index.php\/2022\/04\/20\/entenda-como-funciona-um-purificador-de-agua\/."},{"issue":"6","key":"pone.0280695.ref016","doi-asserted-by":"crossref","first-page":"3433","DOI":"10.1128\/AAC.02582-15","article-title":"Derivatives against Leishmania amazonensis","volume":"60","author":"V Kaplum","year":"2016","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0280695.ref017","first-page":"1","article-title":"Leishmaniasis: A review.","volume":"6","author":"R Arenas","year":"2017","journal-title":"F1000Research."},{"issue":"3","key":"pone.0280695.ref018","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/j.det.2015.03.018","article-title":"New World and Old World Leishmania Infections: A Practical Review.","volume":"33","author":"I Kevric","year":"2015","journal-title":"Dermatol Clin."},{"issue":"9","key":"pone.0280695.ref019","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pntd.0009742","article-title":"Human leishmaniasis vaccines: Use cases, target population and potential global demand.","volume":"15","author":"S Malvolti","year":"2021","journal-title":"PLoS Negl Trop Dis"},{"issue":"1\u20133","key":"pone.0280695.ref020","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.molstruc.2009.08.037","article-title":"The effects of magnetic fields on water molecular hydrogen bonds","volume":"938","author":"R Cai","year":"2009","journal-title":"J Mol Struct"},{"issue":"5674","key":"pone.0280695.ref021","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1126\/science.1096037","article-title":"Infrared spectroscopic evidence for protonated water clusters forming nanoscale cages","volume":"304","author":"M Miyazaki","year":"2004","journal-title":"Science (80-)."},{"issue":"6","key":"pone.0280695.ref022","doi-asserted-by":"crossref","first-page":"1578","DOI":"10.1093\/jac\/dkw012","article-title":"Drug permeation and barrier damage in Leishmania-infected mouse skin","volume":"71","author":"K Van Bocxlaer","year":"2016","journal-title":"J Antimicrob Chemother"},{"issue":"3","key":"pone.0280695.ref023","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1177\/0960327120952151","article-title":"Toxicity, mutagenicity and stability assessment of simply produced electrolyzed water as a wound healing agent in vitro.","volume":"40","author":"R Reis","year":"2021","journal-title":"Hum Exp Toxicol"},{"issue":"4","key":"pone.0280695.ref024","doi-asserted-by":"crossref","first-page":"160","DOI":"10.4103\/2045-9912.248267","article-title":"Daily ingestion of alkaline electrolyzed water containing hydrogen influences human health, including gastrointestinal symptoms.","volume":"8","author":"Y Tanaka","year":"2018","journal-title":"Med Gas Res"},{"key":"pone.0280695.ref025","volume-title":"Hecking the occurrence of changes physical and chemical and molecular water when posted a magnetic field: analysis of statistical relevance","author":"JA Elias","year":"2015"},{"issue":"7","key":"pone.0280695.ref026","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0159763","article-title":"Effect of modified alkaline supplementation on syngenic melanoma growth in CB57\/BL mice.","volume":"11","author":"T Azzarito","year":"2016","journal-title":"PLoS One."},{"issue":"4","key":"pone.0280695.ref027","doi-asserted-by":"crossref","first-page":"1557","DOI":"10.1007\/s00436-015-4890-4","article-title":"Propolis reduces Leishmania amazonensis-induced inflammation in the liver of BALB\/c mice","volume":"115","author":"SS da Silva","year":"2016","journal-title":"Parasitol Res"},{"issue":"3","key":"pone.0280695.ref028","first-page":"1284","article-title":"Effects of alkaline water intake on health: a systematic literature review.","volume":"4","author":"Hamirudin AH Tajudin ZMHM","year":"2020","journal-title":"Inter J All Heal Sci"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0280695","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,6]],"date-time":"2023-07-06T17:34:48Z","timestamp":1688664888000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0280695"}},"subtitle":[],"editor":[{"given":"Colin","family":"Johnson","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2023,7,6]]},"references-count":28,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2023,7,6]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0280695","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,6]]}}}