{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T15:05:36Z","timestamp":1758121536076,"version":"3.40.5"},"reference-count":32,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T00:00:00Z","timestamp":1675728000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Although nitric oxide (NO) is a known factor that regulates the bone physiology, few and discordant results have been obtained in human studies evaluating the effect of nitrates on bone health. We investigated for the relationship between serum NOx level and incident osteoporotic fracture rate prospectively in a cohort consisting of Japanese women. A total of 871 subjects (67.5 \u00b1 10.8 y\/o) were analyzed. During the observation period (8.8 \u00b1 7.2 yrs), incident osteoporotic fractures occurred in 267 participants (209 vertebral fractures, 57 long-bone fractures, and 1 both types). Hazard ratio, by the Cox proportional hazards model, of serum NOx for incident fracture was 0.64 (95% confidence interval 0.53\u20130.78, p &lt; 0.001) after adjustment for baseline age (1.13, 1.06\u20131.21, p &lt; 0.001), lumbar bone mineral density (L-BMD; 0.85, 0.78\u20130.92, p &lt; 0.001), presence of prevalent fracture (3.27, 2.49\u20134.32, p &lt; 0.001), and treatment of osteoporosis (0.70, 0.53\u20130.92, p = 0.010). The relationships between serum level of NOx and bone-related parameters were examined by multiple regression analysis; body mass index (p &lt; 0.001) and L-BMD (p = 0.011) were significantly associated with serum NOx level. These results suggest that the low circulating NOx is one of the independent predictors for osteoporotic fracture occurrence in postmenopausal women.<\/jats:p>","DOI":"10.1371\/journal.pone.0280854","type":"journal-article","created":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T18:30:55Z","timestamp":1675794655000},"page":"e0280854","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Nitric oxide is associated with fracture risk in Japanese women"],"prefix":"10.1371","volume":"18","author":[{"given":"Masataka","family":"Shiraki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuhiko","family":"Kuroda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaki","family":"Nakano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3911-7180","authenticated-orcid":true,"given":"Yukio","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuru","family":"Saito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomohiko","family":"Urano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,2,7]]},"reference":[{"key":"pone.0280854.ref001","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1172\/JCI11468","article-title":"The mechanisms of estrogen regulation of bone resorption","volume":"106","author":"BL Riggs","year":"2000","journal-title":"J Clin Invest"},{"key":"pone.0280854.ref002","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1056\/NEJMoa0809493","article-title":"Denosumab for prevention of fractures in postmenopausal women with osteoporosis","volume":"361","author":"SR Cummings","year":"2009","journal-title":"N Engl J Med"},{"key":"pone.0280854.ref003","doi-asserted-by":"crossref","first-page":"3324","DOI":"10.1073\/pnas.0408742102","article-title":"Regulation of osteoblastogenesis and bone mass","volume":"102","author":"CN Bennett","year":"2005","journal-title":"Proc Natl Acad Sci USA"},{"key":"pone.0280854.ref004","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1002\/jcb.240570305","article-title":"Bone cell function, regulation, and communication: A role for nitric oxide","volume":"57","author":"P Collin-Osdoby","year":"1995","journal-title":"J Cellular Biochem"},{"key":"pone.0280854.ref005","doi-asserted-by":"crossref","first-page":"5835","DOI":"10.1073\/pnas.1804932115","article-title":"Oxygen radicals, nitric oxide, and peroxynitrite: Redox pathway in molecular medicine","volume":"115","author":"R. 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