{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T11:11:07Z","timestamp":1782990667042,"version":"3.54.5"},"reference-count":131,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2023,3,29]],"date-time":"2023-03-29T00:00:00Z","timestamp":1680048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Trematode infection of the second intermediate hosts can lead to changes in their fitness and, as a result, a change in the invasion rate of animal communities. It is especially pronounced during the invasion of parasite species that reduce activity due to the manipulation of hosts through the changes of their morphology and physiology. One of these cases is an anomaly P syndrome hotspot found in some populations of water frogs and toads in Europe caused by the trematode<jats:italic>Strigea robusta<\/jats:italic>metacercariae. The occurrence of pathogen and their participation in ecosystems are intrigues questions in the anomaly P phenomenon, as well as the role of planorbid snails that serve as the first intermediate hosts for many trematode species. Herein, we focused on trematodes spectra from planorbid snails and amphibians from the anomaly P hosts with the aim to undetected interactions between the pathways of parasites. Emerging cercariae of 6802 planorbid snails of dominant species (<jats:italic>Planorbarius corneus<\/jats:italic>,<jats:italic>Planorbis planorbis<\/jats:italic>, and<jats:italic>Anisus<\/jats:italic>spp.) were detected by both morphological and molecular methods in seven waterbodies in Privolzhskaya Lesostep Nature Reserve (Russia). A total of 95 sequences of 18 species were received, and 48 sequences were unique and did not present in any genetic databases. The 18 species of trematodes from snails and 14 species of trematodes from amphibian hosts (<jats:italic>Pelophylax ridibundus<\/jats:italic>; Ranidae; Anura) were detected. Three species (<jats:italic>Echinostoma nasincovae<\/jats:italic>,<jats:italic>Tylodelphys circibuteonis<\/jats:italic>and<jats:italic>Australapatemon burti<\/jats:italic>) was new for the trematode fauna of the Middle Volga River region and Russia as a whole. Eleven species of parasitic flatworms have amphibians in their life cycles and nine species used amphibians as metacercariae hosts:<jats:italic>Echinostoma nasincovae<\/jats:italic>,<jats:italic>E<\/jats:italic>.<jats:italic>miyagawai<\/jats:italic>,<jats:italic>Echinoparyphium recurvatum<\/jats:italic>,<jats:italic>Tylodelphys circibuteonis<\/jats:italic>,<jats:italic>Neodiplostomum spathula<\/jats:italic>,<jats:italic>Paralepoderma cloacicola<\/jats:italic>,<jats:italic>Macrodera longicollis<\/jats:italic>,<jats:italic>Strigea robusta<\/jats:italic>, and<jats:italic>Strigea strigis<\/jats:italic>. The occurrence of trematode species from planorbid mollusks and frogs were compared.<\/jats:p>","DOI":"10.1371\/journal.pone.0281740","type":"journal-article","created":{"date-parts":[[2023,3,29]],"date-time":"2023-03-29T17:42:04Z","timestamp":1680111724000},"page":"e0281740","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":16,"title":["Diversity of trematodes from the amphibian anomaly P hotspot: Role of planorbid snails"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8400-6826","authenticated-orcid":true,"given":"Anton O.","family":"Svinin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor V.","family":"Chikhlyaev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2927-406X","authenticated-orcid":true,"given":"Ivan W.","family":"Bashinskiy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vitaly V.","family":"Osipov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonid A.","family":"Neymark","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander Yu.","family":"Ivanov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara G.","family":"Stoyko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Polina I.","family":"Chernigova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Polina K.","family":"Ibrogimova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Spartak N.","family":"Litvinchuk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oleg A.","family":"Ermakov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,3,29]]},"reference":[{"key":"pone.0281740.ref001","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1038\/nature06970","article-title":"Ecosystem energetic implications of parasite and free-living biomass in three estuaries","volume":"454","author":"AM Kuris","year":"2008","journal-title":"Nature"},{"key":"pone.0281740.ref002","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.tree.2008.08.007","article-title":"The ecological significance of manipulative parasites","volume":"24","author":"T Lef\u00e8vre","year":"2009","journal-title":"Trends in Ecology & Evolution"},{"key":"pone.0281740.ref003","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1111\/j.1461-0248.2008.01174.x","article-title":"Parasites in food webs: the ultimate missing links: Parasites in food webs","volume":"11","author":"KD Lafferty","year":"2008","journal-title":"Ecology Letters"},{"key":"pone.0281740.ref004","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1111\/j.1600-0706.2013.00243.x","article-title":"Parasites as prey in aquatic food webs: implications for predator infection and parasite transmission","author":"DW Thieltges","year":"2013","journal-title":"Oikos"},{"key":"pone.0281740.ref005","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1038\/nature09575","article-title":"Impacts of biodiversity on the emergence and transmission of infectious diseases","volume":"468","author":"F Keesing","year":"2010","journal-title":"Nature"},{"key":"pone.0281740.ref006","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1111\/eva.12294","article-title":"Understanding the ecology and evolution of host-parasite interactions across scales","volume":"9","author":"RM Penczykowski","year":"2016","journal-title":"Evol Appl"},{"key":"pone.0281740.ref007","doi-asserted-by":"crossref","DOI":"10.1093\/oso\/9780195084412.001.0001","volume-title":"Parasites and the behavior of animals","author":"J Moore","year":"2002"},{"key":"pone.0281740.ref008","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1186\/s13071-021-04796-1","article-title":"Host\u2013multiparasite interactions in amphibians: a review","volume":"14","author":"D Herczeg","year":"2021","journal-title":"Parasites Vectors"},{"key":"pone.0281740.ref009","first-page":"161","article-title":"Combined effect of temperature and Wolbachia infection on the fitness of Drosophila suzukii","volume":"71","author":"N Saeed","year":"2018","journal-title":"Bulletin of Insectology"},{"key":"pone.0281740.ref010","doi-asserted-by":"crossref","DOI":"10.1002\/ecs2.3670","article-title":"Infection prevalence and density of a pathogenic trematode parasite decrease with stream order along a river continuum","volume":"12","author":"LP Falke","year":"2021","journal-title":"Ecosphere"},{"key":"pone.0281740.ref011","article-title":"Cooperation and conflict in host manipulation: interactions among macro-parasites and micro-organisms","volume":"5","author":"F C\u00e9zilly","year":"2014","journal-title":"Front Microbiol"},{"key":"pone.0281740.ref012","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/S0020-7519(03)00002-X","article-title":"Host sharing and host manipulation by larval helminths in shore crabs: cooperation or conflict?","volume":"33","author":"R Poulin","year":"2003","journal-title":"International Journal for Parasitology"},{"key":"pone.0281740.ref013","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1038\/nature11883","article-title":"Biodiversity decreases disease through predictable changes in host community competence","volume":"494","author":"PTJ Johnson","year":"2013","journal-title":"Nature"},{"key":"pone.0281740.ref014","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/s11230-007-9127-1","article-title":"Larval trematodes (Digenea) of planorbid snails (Gastropoda: Pulmonata) in Central Europe: a survey of species and key to their identification","volume":"69","author":"A Falt\u00fdnkov\u00e1","year":"2008","journal-title":"Syst Parasitol"},{"key":"pone.0281740.ref015","doi-asserted-by":"crossref","first-page":"310","DOI":"10.2478\/s11686-007-0050-z","article-title":"Furcocercous cercariae (Trematoda) from freshwater snails in Central Finland","volume":"52","author":"A Falt\u00fdnkov\u00e1","year":"2007","journal-title":"Acta Parasitol"},{"key":"pone.0281740.ref016","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1051\/parasite\/2007141039","article-title":"Larval trematodes (Digenea) of the great pond snail, Lymnaea stagnalis (L.), (Gastropoda, Pulmonata) in Central Europe: a survey of species and key to their identification","volume":"14","author":"A Falt\u00fdnkov\u00e1","year":"2007","journal-title":"Parasite"},{"key":"pone.0281740.ref017","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/s00436-010-2047-z","article-title":"Small-scale to large-scale and back: larval trematodes in Lymnaea stagnalis and Planorbarius corneus in Central Europe","volume":"108","author":"R Brown","year":"2011","journal-title":"Parasitol Res"},{"key":"pone.0281740.ref018","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1186\/s13071-020-04536-x","article-title":"Trematode diversity reflecting the community structure of Danish freshwater systems: molecular clues","volume":"14","author":"Y Duan","year":"2021","journal-title":"Parasites Vectors"},{"key":"pone.0281740.ref019","doi-asserted-by":"crossref","first-page":"293","DOI":"10.2478\/s11687-014-0244-5","article-title":"Larval trematodes in bithyniid snails (Gastropoda: Bithyniidae) in the lake-rivers systems from the steppe zone (The West Siberian Plain, Russia)","volume":"51","author":"E. Serbina","year":"2014","journal-title":"Helminthologia"},{"key":"pone.0281740.ref020","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.ijpara.2016.12.008","article-title":"Molecular analyses reveal high species diversity of trematodes in a sub-Arctic lake","volume":"47","author":"M Sold\u00e1nov\u00e1","year":"2017","journal-title":"International Journal for Parasitology"},{"key":"pone.0281740.ref021","doi-asserted-by":"crossref","first-page":"2694","DOI":"10.1038\/s41598-020-59548-5","article-title":"Hidden parasite diversity in a European freshwater system","volume":"10","author":"C Selbach","year":"2020","journal-title":"Sci Rep"},{"key":"pone.0281740.ref022","doi-asserted-by":"crossref","first-page":"9031","DOI":"10.1073\/pnas.95.15.9031","article-title":"Chytridiomycosis causes amphibian mortality associated with population declines in the rain forests of Australia and Central America","volume":"95","author":"L Berger","year":"1998","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"pone.0281740.ref023","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1146\/annurev.ecolsys.30.1.133","article-title":"Global Amphibian Declines: A Problem in Applied Ecology","volume":"30","author":"RA Alford","year":"1999","journal-title":"Annu Rev Ecol Syst"},{"issue":"2","key":"pone.0281740.ref024","first-page":"25","article-title":"Declining amphibian populations: a global phenomenon in conservation biology","volume":"24","author":"T. Gardner","year":"2001","journal-title":"Animal Biodiversity and Conservation"},{"key":"pone.0281740.ref025","first-page":"437","article-title":"Amphibian Population Declines","volume":"57","author":"AR Blaustein","year":"2007","journal-title":"Evolutionary Considerations. BioScience"},{"key":"pone.0281740.ref026","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1242\/jeb.040865","article-title":"The cause of global amphibian declines: a developmental endocrinologist\u2019s perspective","volume":"213","author":"TB Hayes","year":"2010","journal-title":"Journal of Experimental Biology"},{"key":"pone.0281740.ref027","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1111\/j.1749-6632.2010.05909.x","article-title":"The complexity of amphibian population declines: understanding the role of cofactors in driving amphibian losses: The complexity of amphibian population declines","volume":"1223","author":"AR Blaustein","year":"2011","journal-title":"Annals of the New York Academy of Sciences"},{"key":"pone.0281740.ref028","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1007\/s11284-010-0702-6","article-title":"Global stressors and the global decline of amphibians: tipping the stress immunocompetency axis","volume":"26","author":"JM Kiesecker","year":"2011","journal-title":"Ecol Res"},{"key":"pone.0281740.ref029","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s40665-017-0034-7","article-title":"Drivers of amphibian declines: effects of ultraviolet radiation and interactions with other environmental factors","volume":"4","author":"LA Alton","year":"2017","journal-title":"Clim Chang Responses"},{"key":"pone.0281740.ref030","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.1126\/science.aav0379","article-title":"Amphibian fungal panzootic causes catastrophic and ongoing loss of biodiversity","volume":"363","author":"BC Scheele","year":"2019","journal-title":"Science"},{"key":"pone.0281740.ref031","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1038\/s41579-020-0335-x","article-title":"Chytrid fungi and global amphibian declines","volume":"18","author":"MC Fisher","year":"2020","journal-title":"Nat Rev Microbiol"},{"key":"pone.0281740.ref032","first-page":"210","article-title":"Heavy metacercariae infestation (Parastrigea robusta) promotes the decline of a smooth newt population (Lissotriton vulgaris)","volume":"54","author":"U Sinsch","year":"2018","journal-title":"Salamandra"},{"key":"pone.0281740.ref033","doi-asserted-by":"crossref","first-page":"739","DOI":"10.3390\/d14090739","article-title":"The Adaptive Host Manipulation Hypothesis: Parasites Modify the Behaviour, Morphology, and Physiology of Amphibians","volume":"14","author":"I Hernandez-Caballero","year":"2022","journal-title":"Diversity"},{"key":"pone.0281740.ref034","volume-title":"Les anomalies des Amphibiens Anoures","author":"J. Rostand","year":"1958"},{"key":"pone.0281740.ref035","volume-title":"Les \u00e9tangs \u00e0 monstres. Histoire d\u2019une recherche (1947\u20131970)","author":"J. Rostand","year":"1971"},{"key":"pone.0281740.ref036","first-page":"49","article-title":"Rostand\u2019s anomaly P in Palaearctic green frogs (Pelophylax) and similar anomalies in amphibians","volume":"25","author":"A. Dubois","year":"2017","journal-title":"Mertensiella"},{"key":"pone.0281740.ref037","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1186\/s13071-020-04256-2","article-title":"Strigea robusta causes polydactyly and severe forms of Rostand\u2019s anomaly P in water frogs","volume":"13","author":"AO Svinin","year":"2020","journal-title":"Parasites Vectors"},{"key":"pone.0281740.ref038","first-page":"31","article-title":"First record of the Jean Rostand\u2019s \u201canomaly P\u201d in the marsh frog, Pelophylax ridibundus, in central Russia","volume":"37","author":"AO Svinin","year":"2019","journal-title":"Alytes"},{"key":"pone.0281740.ref039","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.limno.2018.10.005","article-title":"Forest-steppe oxbows in limnophase\u2014Abiotic features and biodiversity","volume":"74","author":"IV Bashinskiy","year":"2019","journal-title":"Limnologica"},{"key":"pone.0281740.ref040","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1007\/s13157-022-01625-8","article-title":"The more Diverse Beaver Ponds are Better\u2013a Case Study of Mollusc Communities of Steppe Streams","volume":"42","author":"IW Bashinskiy","year":"2022","journal-title":"Wetlands"},{"key":"pone.0281740.ref041","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1134\/S1995425520060037","article-title":"Structure and Dynamics of Mollusk Communities of Small Oxbow Lakes and the Determining Factors (the Khoper River Valley, Penza Oblast)","volume":"13","author":"IV Bashinskiy","year":"2020","journal-title":"Contemp Probl Ecol"},{"key":"pone.0281740.ref042","volume-title":"Mollyuski presnykh i solonovatykh vod SSSR (Mollusks of Freshwaters and Saline Waters of USSR)","author":"VI Zhadin","year":"1952"},{"key":"pone.0281740.ref043","unstructured":"Starobogatov YI, Prozorova LA, Bogatov VV, Saenko, EM. The Guide for Identification of Freshwater Invertebrates of Russia and Adjacent Territories, Vol. 6: Mollusks, Polychaete, and Nemertean. St. Petersburg: Nauka; 2004."},{"key":"pone.0281740.ref044","unstructured":"Combes C. Atlas mondial des cercaires. Paris: Editions du Mus\u00e9um national d\u2019histoire naturelle; 1980."},{"issue":"Supl. 1","key":"pone.0281740.ref045","first-page":"1","article-title":"Species composition and distributional peculiarities of green frogs (Pelophylax esculentus complex) in protected areas of the Middle Volga Region (Russia)","volume":"3","author":"AI Fayzulin","year":"2018","journal-title":"Nature Conservation Research"},{"key":"pone.0281740.ref046","volume-title":"Amphibian Helminths of the USSR Fauna","author":"KM Ryzhikov","year":"1980"},{"key":"pone.0281740.ref047","volume-title":"Metacercariae of Trematodes- Parasites of Freshwater Aquatic Organisms in Central Russia","author":"VP Sudarikov","year":"2002"},{"key":"pone.0281740.ref048","doi-asserted-by":"crossref","first-page":"4692","DOI":"10.1093\/nar\/25.22.4692","article-title":"Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques","volume":"25","author":"SM Aljanabi","year":"1997","journal-title":"Nucleic Acids Research"},{"key":"pone.0281740.ref049","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1023\/A:1025546001611","article-title":"Molecular phylogenetic analysis of the Microphalloidea Ward, 1901 (Trematoda: Digenea)","volume":"56","author":"VV Tkach","year":"2003","journal-title":"Syst Parasitol"},{"key":"pone.0281740.ref050","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1645\/GE-465R.1","article-title":"A molecular phylogenetic study of the genus Ribeiroia (Digenea): trematodes known to cause limb malformations in amphibians","volume":"91","author":"WD Wilson","year":"2005","journal-title":"Journal of Parasitology"},{"key":"pone.0281740.ref051","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1006\/mpev.1995.1011","article-title":"A molecular phylogeny of the human schistosomes","volume":"4","author":"J Bowles","year":"1995","journal-title":"Molecular Phylogenetics and Evolution"},{"key":"pone.0281740.ref052","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1093\/oxfordjournals.molbev.a026098","article-title":"Reticulate evolution and the origins of ribosomal internal transcribed spacer diversity in apomictic Meloidogyne","volume":"16","author":"A Hugall","year":"1999","journal-title":"Molecular Biology and Evolution"},{"key":"pone.0281740.ref053","first-page":"170","article-title":"Molecular and morphological evidence for close phylogenetic affinities of the genera Macrodera, Leptophallus, Metaleptophallus and Paralepoderma (Digenea, Plagiorchiata)","volume":"44","author":"V Tkach","year":"1999","journal-title":"Acta Parasitologica"},{"key":"pone.0281740.ref054","first-page":"83","article-title":"Phylogenetic analysis of the suborder Plagiorchiata (Platyhelminthes, Digenea) based on partial lsrDNA sequences","volume":"30","author":"V Tkach","year":"2000","journal-title":"International Journal for Parasitology"},{"key":"pone.0281740.ref055","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1645\/0022-3395(2001)087[1433:PABRAS]2.0.CO;2","article-title":"Phylogenetic and Biogeographical Relationships among Some Holarctic Frog Lung Flukes (Digenea: Haematoloechidae)","volume":"87","author":"SD Snyder","year":"2001","journal-title":"The Journal of Parasitology"},{"key":"pone.0281740.ref056","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1016\/j.ijpara.2005.02.014","article-title":"Molecular-genetic analyses reveal cryptic species of trematodes in the intertidal gastropod, Batillaria cumingi (Crosse)","volume":"35","author":"O Miura","year":"2005","journal-title":"International Journal for Parasitology"},{"key":"pone.0281740.ref057","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic Local Alignment Search Tool","volume":"215","author":"SF Altschul","year":"1990","journal-title":"Journal of Molecular Biology"},{"key":"pone.0281740.ref058","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1093\/molbev\/msu300","article-title":"IQ-TREE: A Fast and Effective Stochastic Algorithm for Estimating Maximum-Likelihood Phylogenies","volume":"32","author":"L-T Nguyen","year":"2015","journal-title":"Molecular Biology and Evolution"},{"key":"pone.0281740.ref059","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1038\/nmeth.4285","article-title":"ModelFinder: fast model selection for accurate phylogenetic estimates","volume":"14","author":"S Kalyaanamoorthy","year":"2017","journal-title":"Nat Methods"},{"key":"pone.0281740.ref060","doi-asserted-by":"crossref","first-page":"W232","DOI":"10.1093\/nar\/gkw256","article-title":"W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis","volume":"44","author":"J Trifinopoulos","year":"2016","journal-title":"Nucleic Acids Res"},{"key":"pone.0281740.ref061","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1093\/molbev\/mst024","article-title":"Ultrafast Approximation for Phylogenetic Bootstrap","volume":"30","author":"BQ Minh","year":"2013","journal-title":"Molecular Biology and Evolution"},{"key":"pone.0281740.ref062","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1093\/molbev\/msx281","article-title":"UFBoot2: Improving the Ultrafast Bootstrap Approximation","volume":"35","author":"DT Hoang","year":"2018","journal-title":"Molecular Biology and Evolution"},{"key":"pone.0281740.ref063","doi-asserted-by":"crossref","first-page":"1687","DOI":"10.1007\/s00436-021-07082-2","article-title":"Cryptic speciation among Tylodelphys spp.: the major helminth pathogens of fish and amphibians","volume":"120","author":"P Heneberg","year":"2021","journal-title":"Parasitol Res"},{"key":"pone.0281740.ref064","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1093\/zoolinnean\/zlab114","article-title":"Molecular phylogeny supports invalidation of Didelphodiplostomum and Pharyngostomoides (Digenea: Diplostomidae) and reveals a Tylodelphys from mammals","volume":"196","author":"TJ Achatz","year":"2022","journal-title":"Zool. J. Linn. Soc"},{"key":"pone.0281740.ref065","doi-asserted-by":"crossref","first-page":"102033","DOI":"10.1016\/j.parint.2019.102033","article-title":"Paraphyly of Conodiplostomum Dubois, 1937","volume":"76","author":"P Heneberg","year":"2020","journal-title":"Parasitology International"},{"key":"pone.0281740.ref066","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1186\/s13071-020-04538-9","article-title":"Leeches as the intermediate host for strigeid trematodes: genetic diversity and taxonomy of the genera Australapatemon Sudarikov, 1959 and Cotylurus Szidat, 1928","volume":"14","author":"E Pyrka","year":"2021","journal-title":"Parasites Vectors"},{"key":"pone.0281740.ref067","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.parint.2013.11.012","article-title":"Morphological and molecular differentiation of Parastrigea (Trematoda: Strigeidae) from Mexico, with the description of a new species","volume":"63","author":"DI Hern\u00e1ndez-Mena","year":"2014","journal-title":"Parasitology International"},{"key":"pone.0281740.ref068","doi-asserted-by":"crossref","first-page":"588","DOI":"10.11646\/zootaxa.4132.4.10","article-title":"First molecular identification of Australapatemon burti (Miller, 1923) (Trematoda: Digenea: Strigeidae) from an intermediate host Radix labiata (Rossmaessler) (Gastropoda: Lymnaeidae) in Europe","volume":"4132","author":"OV Aksenova","year":"2016","journal-title":"Zootaxa"},{"key":"pone.0281740.ref069","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1002\/jez.2599","article-title":"Strigea robusta (Digenea: Strigeidae) infection effects on the gonadal structure and limb malformation in toad early development","volume":"337","author":"AO Svinin","year":"2022","journal-title":"J Exp Zool A Ecol Integr Physiol"},{"key":"pone.0281740.ref070","article-title":"Effects of minimum Strigea robusta (Digenea: Strigeidae) cercariae doses and localization of cysts on the anomaly P manifestation in Pelophylax lessonae (Anura: Ranidae) tadpoles","author":"AO Svinin","year":"2023","journal-title":"Parasitol. Res"},{"key":"pone.0281740.ref071","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1017\/S003118202200083X","article-title":"Hidden in plain sight: Novel molecular data reveal unexpected genetic diversity among paramphistome parasites (Digenea: Paramphistomoidea) of European water frogs","volume":"149","author":"M Benovics","year":"2022","journal-title":"Parasitology"},{"key":"pone.0281740.ref072","first-page":"733","article-title":"Phylogeny and classification of the Digenea (Platyhelminthes: Trematoda)","volume":"33","author":"PD Olson","year":"2003","journal-title":"Int J Parasitol"},{"key":"pone.0281740.ref073","first-page":"520","article-title":"Echinostoma \u201crevolutum\u201d (Digenea: Echinostomatidae) species complex revisited: species delimitation based on novel molecular and morphological data gathered in Europe","volume":"7","author":"S Georgieva","year":"2014","journal-title":"Parasites Vectors"},{"key":"pone.0281740.ref074","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11230-014-9530-3","article-title":"A re-assessment of species diversity within the \u2018revolutum\u2019 group of Echinostoma Rudolphi, 1809 (Digenea: Echinostomatidae) in Europe","volume":"90","author":"A Falt\u00fdnkov\u00e1","year":"2015","journal-title":"Syst Parasitol"},{"key":"pone.0281740.ref075","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1186\/1756-3305-6-64","article-title":"New cryptic species of the \u2018revolutum\u2019 group of Echinostoma (Digenea: Echinostomatidae) revealed by molecular and morphological data","volume":"6","author":"S Georgieva","year":"2013","journal-title":"Parasit Vectors"},{"key":"pone.0281740.ref076","first-page":"287","article-title":"Diversity of trematode larvae in gastropod molluscs in water bodies of Belarus","volume":"45","author":"LN Akimova","year":"2011","journal-title":"Parazitologiya"},{"key":"pone.0281740.ref077","doi-asserted-by":"crossref","first-page":"283","DOI":"10.3897\/herpetozoa.35.e95928","article-title":"The incidence of the anomaly P syndrome in water frogs (Anura, Ranidae, Pelophylax) from the Middle Volga River (Russia)","volume":"35","author":"AO Svinin","year":"2022","journal-title":"Herpetozoa"},{"key":"pone.0281740.ref078","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/j.ijpara.2011.05.001","article-title":"Small worms, big appetites: ratios of different functional morphs in relation to interspecific competition in trematode parasites","volume":"41","author":"TL Leung","year":"2011","journal-title":"Int J Parasitol"},{"key":"pone.0281740.ref079","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.ijpara.2016.10.003","article-title":"Trematodes with a reproductive division of labour: heterophyids also have a soldier caste and early infections reveal how colonies become structured","volume":"47","author":"AE Garcia-Vedrenne","year":"2017","journal-title":"Int J Parasitol"},{"key":"pone.0281740.ref080","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1038\/206422a0","article-title":"Antagonism between two species of larval trematodes in the same snail","volume":"206","author":"KJ Lie","year":"1965","journal-title":"Nature"},{"key":"pone.0281740.ref081","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/S0065-308X(08)60175-X","article-title":"Intramolluscan intertrematode antagonism: a review of factors influencing the host-parasite system and its possible role in biological control","volume":"10","author":"HK Lim","year":"1972","journal-title":"Advances in Parasitology"},{"key":"pone.0281740.ref082","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.2307\/3279371","article-title":"Predation by echinostome rediae upon schistosome sporocyst in vitro","volume":"61","author":"PF Basch","year":"1975","journal-title":"J. Parasitol"},{"key":"pone.0281740.ref083","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/0014-4894(73)90038-6","article-title":"Larval trematode antagonism: principles and possible application as a control method","volume":"33","author":"J. Lie","year":"1973","journal-title":"Exp. Parasitol"},{"key":"pone.0281740.ref084","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1017\/S0031182000053634","article-title":"Trematodes: antagonism between species and sterilizing effects on snails in biological control","volume":"84","author":"C. Combes","year":"1982","journal-title":"Parasitology"},{"key":"pone.0281740.ref085","unstructured":"Kirillov AA, Kirillova NYu, Chikhlyaev IV. Trematodes of land vertebrates of Middle Volga region. Togliatti: Cassandra.; 2012."},{"key":"pone.0281740.ref086","first-page":"47","article-title":"Trematodes of birds (Aves) from the Middle Volga region. 1. Orders Brachylaimidae, Cyclocoelida, Echinostomatida, Notocotylida, and Opisthorchiida","volume":"47","author":"A Kirillov","year":"2013","journal-title":"Parazitologiia"},{"key":"pone.0281740.ref087","doi-asserted-by":"crossref","first-page":"434","DOI":"10.7589\/0090-3558-29.3.434","article-title":"Experimental infection of domestic ducks and rodents by Notocotylus attenuatus (Trematoda: Notocotylidae)","volume":"29","author":"K Graczyk","year":"1993","journal-title":"Journal of Wildlife Diseases"},{"key":"pone.0281740.ref088","first-page":"29","article-title":"Dynamics of foci of bird notocotylidosis in the ecosystem of Lake Chany (Western Siberia) in the last 80 years","volume":"3","author":"EA Serbina","year":"2015","journal-title":"Russian Journal of Parasitology"},{"key":"pone.0281740.ref089","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1645\/GE-3515.1","article-title":"An approach to revealing blood fluke life cycles, taxonomy, and diversity: provision of key reference data including DNA sequence from single life cycle stages","volume":"92","author":"SV Brant","year":"2006","journal-title":"Journal of Parasitology"},{"key":"pone.0281740.ref090","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1111\/j.1365-2427.2010.02545.x","article-title":"Snails, waterfowl and cercarial dermatitis: Bird schistosomes","volume":"56","author":"P Hor\u00e1k","year":"2011","journal-title":"Freshwater Biology"},{"key":"pone.0281740.ref091","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1007\/s00436-016-5359-9","article-title":"Having bird schistosomes in mind\u2014the first detection of Bilharziella polonica (Kowalewski 1895) in the bird neural system","volume":"116","author":"H Pr\u00fcter","year":"2017","journal-title":"Parasitol Res"},{"key":"pone.0281740.ref092","first-page":"69","article-title":"Bird schistosomesfrom freshwater snails in Austria, with some notes on current problems (Digenea, Schistosomatidae)","volume":"21","author":"J Dvorak","year":"1999","journal-title":"Mitteilungen der \u00d6sterreichischen Gesellschaft f\u00fcr Tropenmedizin und Parasitologie"},{"key":"pone.0281740.ref093","first-page":"261","article-title":"Observations on the life cycle of Diplodiscus subclavatus (Pallas, 1760) (Trematoda, Diplodiscidae)","volume":"27","author":"B. Grabda-Kazubska","year":"1980","journal-title":"Acta Parasitol\u00f3gica Polonica"},{"key":"pone.0281740.ref094","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1515\/ap-2016-0119","article-title":"A morphological study of Diplodiscus subclavatus (Pallas, 1760) (Trematoda: Diplodiscidae) adults from the accidental host, Viviparus contectus (Millet, 1813) (Caenogastropoda: Viviparidae)","volume":"61","author":"A Cichy","year":"2016","journal-title":"Acta Parasitologica"},{"key":"pone.0281740.ref095","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.1007\/s00436-022-07523-6","article-title":"Frequent infection of urban grass snakes (Natrix natrix) oral cavity with Leptophallus nigrovenosus trematode","volume":"121","author":"B Zaj\u0105c","year":"2022","journal-title":"Parasitol Res"},{"key":"pone.0281740.ref096","article-title":"Helminth fauna of reptiles in the National Park \u00abSmolny\u00bb","volume":"6","author":"A\u0410 Kirillov","year":"2021","journal-title":"Russia. Nature Conservation Research"},{"key":"pone.0281740.ref097","doi-asserted-by":"crossref","first-page":"108","DOI":"10.2478\/s11687-011-0020-8","article-title":"Endoparasitic helminths of water frog complex in Poland: do differences exist between the parental species Pelophylax ridibundus and Pelophylax lessonae, and their natural hybrid Pelophylax esculentus?","volume":"48","author":"M Popio\u0142ek","year":"2011","journal-title":"Helminthologia"},{"key":"pone.0281740.ref098","first-page":"1","article-title":"Helminth Fauna of Green Frogs (Rana esculenta Complex) in the Central Chernozem Territory of Russia","volume":"18","author":"MV Rezvantseva","year":"2011","journal-title":"Russian Journal of Herpetology"},{"key":"pone.0281740.ref099","doi-asserted-by":"crossref","DOI":"10.24189\/ncr.2018.039","article-title":"Ecological analysis of trematodes (Trematoda) of marsh frog Pelophylax ridibundus (Ranidae, Anura) from various habitats of the National Park \u00abSamarskaya Luka\u00bb (Russia)","volume":"3","author":"IV Chikhlyaev","year":"2018","journal-title":"Nat Conserv Res"},{"key":"pone.0281740.ref100","doi-asserted-by":"crossref","first-page":"341","DOI":"10.2478\/s11686-019-00164-3","article-title":"Helminth Species and Infracommunities in Frogs Pelophylax ridibundus and P. esculentus (Amphibia: Ranidae) in Northern Ukraine","volume":"65","author":"Y Kuzmin","year":"2020","journal-title":"Acta Parasit"},{"key":"pone.0281740.ref101","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1654\/1525-2647-83.1.36","article-title":"Helminth Parasites of the Pelophylax esculentus Complex (Anura: Ranidae) in Hortob\u00e1gy National Park (Hungary)","volume":"83","author":"D Herczeg","year":"2016","journal-title":"Comparative Parasitology"},{"key":"pone.0281740.ref102","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1016\/j.parint.2018.07.003","article-title":"Central European Strigeidae Railliet, 1919 (Trematoda: Strigeidida): Molecular and comparative morphological analysis suggests the reclassification of Parastrigea robusta Szidat, 1928 into Strigea Abildgaard, 1790","volume":"67","author":"P Heneberg","year":"2018","journal-title":"Parasitology International"},{"key":"pone.0281740.ref103","first-page":"38","article-title":"Investigation of morphological anomalies and infection by helminths of marsh frogs, Pelophylax ridibundus (Pallas, 1771), in separate populations in the Kiev city","volume":"48","author":"OY Marushchak","year":"2017","journal-title":"Zb prac\u2019 Zool muz (Kiiv)"},{"key":"pone.0281740.ref104","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1007\/s004360050316","article-title":"Experimental infection of Rana pipiens tadpoles with Echinostoma trivolvis cercariae","volume":"83","author":"B Fried","year":"1997","journal-title":"Parasitology Research"},{"key":"pone.0281740.ref105","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1139\/z06-119","article-title":"Impact of eutrophication on wood frog, Rana sylvatica, tadpoles infected with Echinostoma trivolvis cercariae","volume":"84","author":"LK Belden","year":"2006","journal-title":"Canadian Journal of Zoology"},{"key":"pone.0281740.ref106","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1645\/GE-624R.1","article-title":"Effects of atrazine on cercarial longevity, activity, and infectivity","volume":"92","author":"J Koprivnikar","year":"2006","journal-title":"Journal of Parasitology"},{"key":"pone.0281740.ref107","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1645\/GE-1782.1","article-title":"Echinostome Metacercariae Cyst Elimination in Rana clamitans (Green Frog) Tadpoles is Age-dependent","volume":"95","author":"MP Holland","year":"2009","journal-title":"Journal of Parasitology"},{"key":"pone.0281740.ref108","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1645\/GE-2351.1","article-title":"Echinostome-Induced Mortality Varies Across Amphibian Species in the Field","volume":"96","author":"MP Holland","year":"2010","journal-title":"Journal of Parasitology"},{"key":"pone.0281740.ref109","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1645\/GE-1840.1","article-title":"Moderate Echinostoma trivolvis Infection Has No Effects on Physiology and Fitness-Related Traits of Larval Pickerel Frogs (Rana palustris)","volume":"95","author":"SA Orlofske","year":"2009","journal-title":"The Journal of Parasitology"},{"key":"pone.0281740.ref110","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.cbpa.2016.08.002","article-title":"Effects of Echinostoma trivolvis metacercariae infection during development and metamorphosis of the wood frog (Lithobates sylvaticus)","volume":"203","author":"SA Orlofske","year":"2017","journal-title":"Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology"},{"key":"pone.0281740.ref111","doi-asserted-by":"crossref","first-page":"1515","DOI":"10.1017\/S0031182020001092","article-title":"Timing and order of exposure to two echinostome species affect patterns of infection in larval amphibians","volume":"147","author":"LS Billet","year":"2020","journal-title":"Parasitology"},{"key":"pone.0281740.ref112","doi-asserted-by":"crossref","first-page":"17","DOI":"10.3347\/kjp.2019.57.1.17","article-title":"Necrotizing Enteritis Caused by Pharyngostomum cordatum Infection in a Stray Cat","volume":"57","author":"JH Kim","year":"2019","journal-title":"Korean J Parasitol"},{"key":"pone.0281740.ref113","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1126\/science.284.5415.802","article-title":"The effect of trematode infection on amphibian limb development and survivorship","volume":"284","author":"PTJ Johnson","year":"1999","journal-title":"Science"},{"key":"pone.0281740.ref114","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1139\/z00-210","article-title":"Ribeiroia ondatrae (Trematoda: Digenea) infection induces severe limb malformations in western toads (Bufo boreas)","volume":"79","author":"PTJ Johnson","year":"2001","journal-title":"Canadian Journal of Zoology"},{"key":"pone.0281740.ref115","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1890\/0012-9615(2002)072[0151:PROILT]2.0.CO;2","article-title":"Parasite (Ribeiroia ondatrae) infection linked to amphibian malformations in the western United States","volume":"72","author":"PTJ Johnson","year":"2002","journal-title":"Ecological Monographs"},{"key":"pone.0281740.ref116","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/S1471-4922(03)00148-X","article-title":"Amphibian deformities and Ribeiroia infection: An emerging helminthiasis","volume":"19","author":"PTJ Johnson","year":"2003","journal-title":"Trends in Parasitology"},{"key":"pone.0281740.ref117","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1890\/1540-9295(2003)001[0087:TCODA]2.0.CO;2","article-title":"The complexity of deformed amphibians","volume":"1","author":"AR Blaustein","year":"2003","journal-title":"Frontiers in Ecology and the Environment"},{"key":"pone.0281740.ref118","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/S0065-308X(04)57003-3","article-title":"Review of the trematode genus Ribeiroia (Psilostomidae): Ecology, life history and pathogenesis with special emphasis on the amphibian malformation problem","volume":"57","author":"PTJ Johnson","year":"2004","journal-title":"Advances in Parasitology"},{"key":"pone.0281740.ref119","doi-asserted-by":"crossref","first-page":"15781","DOI":"10.1073\/pnas.0707763104","article-title":"Aquatic eutrophication promotes pathogenic infection in amphibians","volume":"104","author":"PTJ Johnson","year":"2007","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"pone.0281740.ref120","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1111\/j.1365-2656.2008.01455.x","article-title":"All hosts are not equal: explaining differential patterns of malformations in an amphibian community","volume":"78","author":"PTJ Johnson","year":"2009","journal-title":"Journal of Animal Ecology"},{"key":"pone.0281740.ref121","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1111\/j.1365-2435.2010.01830.x","article-title":"Critical windows of disease risk: amphibian pathology driven by developmental changes in host resistance and tolerance","volume":"25","author":"PTJ Johnson","year":"2011","journal-title":"Functional Ecology"},{"key":"pone.0281740.ref122","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1111\/j.1461-0248.2011.01730.x","article-title":"Living fast and dying of infection: host life history drives interspecific variation in infection and disease risk","volume":"15","author":"PTJ Johnson","year":"2012","journal-title":"Ecology Letters"},{"key":"pone.0281740.ref123","first-page":"e275","article-title":"Continental-extent patterns in amphibian malformations linked to parasites, chemical contaminants, and their interactions","volume":"24","author":"SE Haas","year":"2018","journal-title":"Global Change Biology"},{"key":"pone.0281740.ref124","first-page":"241","article-title":"Monostome cercariae induced malformations in amphibians: effect of infection at the pre-limb-bud stage tadpoles of Polypedates cruciger Blyth","volume":"38","author":"UA Jayawardena","year":"2010","journal-title":"J Natl Sci Found Sri"},{"key":"pone.0281740.ref125","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1111\/j.1469-7998.2008.00416.x","article-title":"Trematode infection induced malformations in the common hourglass treefrogs","volume":"275","author":"RS Rajakaruna","year":"2008","journal-title":"J Zool"},{"key":"pone.0281740.ref126","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.parint.2013.01.003","article-title":"Malformations and mortality in the Asian common toad induced by exposure to pleurolophocercous cercariae (Trematoda: Cryptogonimidae)","volume":"62","author":"UA Jayawardena","year":"2013","journal-title":"Parasitol Int"},{"key":"pone.0281740.ref127","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/s10393-016-1103-2","article-title":"Combined effects of pesticides and trematode infections on hourglass tree frog Polypedates cruciger","volume":"13","author":"UA Jayawardena","year":"2016","journal-title":"EcoHealth"},{"key":"pone.0281740.ref128","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s40850-017-0022-1","article-title":"Effects of agrochemicals on disease severity of Acanthostomum burminis infections (Digenea: Trematoda) in the Asian common toad, Duttaphrynus melanostictus","volume":"2","author":"UA Jayawardena","year":"2017","journal-title":"BMC Zoology"},{"key":"pone.0281740.ref129","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1134\/S0012496611050073","article-title":"The role of trematode infestation in the specifics of skeleton morphogenesis of Rana arvalis Nilsson, 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