{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T03:00:34Z","timestamp":1789786834613,"version":"4.0.1"},"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2023,2,21]],"date-time":"2023-02-21T00:00:00Z","timestamp":1676937600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Food contamination by <jats:italic>Staphylococcus<\/jats:italic> spp. enterotoxigenic strains is quite common and despite underreporting caused by the short duration of clinical symptoms and lack of medical care, staphylococcal food poisoning is one of the most common Foodborne Diseases (FBD) in the world. This study describes a systematic review protocol with meta-analysis on the prevalence and types of staphylococcal enterotoxins in food, and the profile of contaminated foods.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>The research will be conducted through the selection of studies reporting the analysis of staphylococcal enterotoxins in food contaminated by <jats:italic>Staphylococcus<\/jats:italic> spp. Searches will happen on the following databases: Medline (OVID), GALE, Science Direct, CAB Direct (CABI), Google Scholar, in addition to manual search in the list of references of articles, directory of theses and dissertations, and countries\u2019 health agencies. Reports will be imported into the application Rayyan. Two researchers will independently select studies and extract data, and a third reviewer will solve conflicting decisions. The primary outcome will be the identification of staphylococcal enterotoxins in food, and the secondary outcomes will include staphylococcal enterotoxin types and foods involved. To assess the risk of bias in the studies, the tool developed by the Joanna Briggs Institute (JBI) will be used. For data synthesis, a meta-analysis will be performed. However, in case that is not possible, a narrative synthesis of the most relevant results will be carried out.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Discussion<\/jats:title>\n<jats:p>This protocol will serve as the basis for a systematic review that aims to relate the results of existing studies on the staphylococcal enterotoxin prevalence and types in food, and the profile of the contaminated foods. The results will broaden the perception of food safety risks, highlight existing literature gaps, contribute to the study of the epidemiological profile and may guide the allocation of health resources for the development of preventive measures related.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Systematic review registration<\/jats:title>\n<jats:p>PROSPERO registration number: <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/www.crd.york.ac.uk\/prospero\/display_record.php?ID=CRD42021258223\" xlink:type=\"simple\">CRD42021258223<\/jats:ext-link>.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0282111","type":"journal-article","created":{"date-parts":[[2023,2,22]],"date-time":"2023-02-22T01:53:02Z","timestamp":1677030782000},"page":"e0282111","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["Prevalence of staphylococcal toxin in food contaminated by Staphylococcus spp.: Protocol for a systematic review with meta-analysis"],"prefix":"10.1371","volume":"18","author":[{"given":"Juliana Karla Garcia Ribeiro","family":"Freitas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiane Fernandes de","family":"Assis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thailla Raquel Moura de","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e1udio M\u00e1rcio de Medeiros","family":"Maia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5367-236X","authenticated-orcid":true,"given":"Bruno Jonatan","family":"de Sousa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gidyenne Christiane Bandeira Silva de","family":"Medeiros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa Mont\u2019Alverne Juc\u00e1","family":"Seabra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6884-1023","authenticated-orcid":true,"given":"Karla Suzanne Florentino da Silva","family":"Chaves Damasceno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,2,21]]},"reference":[{"key":"pone.0282111.ref001","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.cnd.2019.12.001","article-title":"Les maladies microbiennes d\u2019origine alimentaire.","volume":"55","author":"F Dubois-Brissonnet","year":"2020","journal-title":"Cah Nutr Diet"},{"key":"pone.0282111.ref002","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1590\/0103-1104202012622","article-title":"Dietas saud\u00e1veis e sustent\u00e1veis no \u00e2mbito do sistema alimentar no s\u00e9culo XXI.","volume":"44","author":"RM Triches","year":"2020","journal-title":"Sa\u00fade em Debate"},{"key":"pone.0282111.ref003","author":"Minist\u00e9rio da Sa\u00fade","year":"2010"},{"key":"pone.0282111.ref004","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pmed.1001923","article-title":"World Health Organization Global Estimates and Regional Comparisons of the Burden of Foodborne Disease in 2010.","volume":"12","author":"AH Havelaar","year":"2015","journal-title":"PLoS Med."},{"key":"pone.0282111.ref005","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1111\/j.1574-6976.2011.00311.x","article-title":"Staphylococcus aureus and its food poisoning toxins: characterization and outbreak investigation","volume":"36","author":"J-A Hennekinne","year":"2012","journal-title":"FEMS Microbiol Rev"},{"key":"pone.0282111.ref006","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1590\/s1678-9946202062005","article-title":"Presence of genes encoding enterotoxins in Staphylococcus aureus isolates recovered from food, food establishment surfaces and cases of foodborne diseases Virginia.","volume":"62","author":"V Machado","year":"2020","journal-title":"Rev Inst Med Trop Sao Paulo"},{"key":"pone.0282111.ref007","article-title":"WHO estimates of the global burden os foodborne diseases. 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