{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T23:16:23Z","timestamp":1793315783110,"version":"4.5.0"},"reference-count":117,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2023,3,3]],"date-time":"2023-03-03T00:00:00Z","timestamp":1677801600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\"><jats:title>Background<\/jats:title><jats:p>Pre-slaughter stress or the welfare condition of food-producing animals (FPAs) and the slaughter practices of slaughterhouse workers (SHWs) are critically important for the safety and quality of meats processed in slaughterhouses (SHs). Consequently, this study determined the pre-slaughter, slaughter, and post-slaughter (PSP) practices of SHWsin four SHs in Southeast, Nigeria; and discussed the impacts on meat quality and safety.<\/jats:p><\/jats:sec><jats:sec id=\"sec002\"><jats:title>Methods<\/jats:title><jats:p>The PSP practices were determined by observation method. Additionally, a structured and validated closed-ended questionnaire was used to determine the knowledge of the SHWs on: the effects of poor welfare (preslaughter stress) on the quality and safety of meats produced, carcass\/meat processing practices and modes of transmission of meat-borne zoonotic pathogens during carcass\/meat processing. Finally, a systematic post-mortem inspection (PMI) was conducted on cattle, pigs and goats slaughtered, and economic losses accruable from condemned carcasses\/meats were estimated.<\/jats:p><\/jats:sec><jats:sec id=\"sec003\"><jats:title>Results<\/jats:title><jats:p>Food-producing animals were transported to the SHs or held in the lairage under inhumane conditions. A pig being conveyed to one of the SHs was seen gasping for air, as it was firmly tied on motorbike at the thoracic and abdominal regions. Fatigued cattle were forcefully dragged on the ground from the lairage to the killing floor. Cattle for slaughter were restrained, held in lateral recumbency and left groaning, due to extreme discomfort, for about one hour before slaughter. Stunning was not performed. Singed pig carcasses were dragged on the ground to the washing point. Although more than 50% of the respondents knew the modes of transmission of meat-borne zoonotic pathogens during meat processing, 71.3% of the SHWs processed carcasses on bare floor, 52.2% used same bowl of water to wash multiple carcasses while 72% did not wear personal protective equipment during meat\/carcass processing. Processed meats were transported to meat shops in an unsanitary conditions, using open vans and tricycles. During the PMI, diseased carcasses\/meats\/organs were detected in 5.7% (83\/1452), 2.1% (21\/1006) and 0.8% (7\/924) of the cattle, pig and goat carcasses inspected, respectively. Gross lesions pathognomonic of bovine tuberculosis, contagious bovine pleuro-pneumonia, fascioliasis and porcine cysticercosis were detected. Consequently, 391,089.2 kg of diseased meat\/organs valued at 978 million Naira (235, 030 USD) were condemned. There were significant associations (p &lt; 0.05) between educational level and the use of personal protective equipment (PPE) during slaughterhouse operations and knowledge that FPAs can harbour zoonotic pathogens (p = &lt; 0.001) transmissible during carcass processing. Similarly, significant association was observed between working experience and use of PPE; and between geographical location of the respondents and knowledge that zoonotic pathogens in animals are transmissible during carcass processing or via the food chain.<\/jats:p><\/jats:sec><jats:sec id=\"sec004\"><jats:title>Conclusion<\/jats:title><jats:p>The findings show that slaughter practices of SHWs have detrimental impacts on the quality and safety of meats processed for human consumption in Southeast, Nigeria. These findings underscore the need to: improve the welfare condition of slaughter-animals, mechanise abattoir operations, train and retrain the SHWs on hygienic carcass\/meat processing practices. There is a need to adopt strict enforcement of food safety laws to promote meat quality, food safety and consequently promote the health of the public.<\/jats:p><\/jats:sec>","DOI":"10.1371\/journal.pone.0282418","type":"journal-article","created":{"date-parts":[[2023,3,3]],"date-time":"2023-03-03T18:27:01Z","timestamp":1677868021000},"page":"e0282418","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":54,"title":["Pre-slaughter, slaughter and post-slaughter practices of slaughterhouse workers in Southeast, Nigeria: Animal welfare, meat quality, food safety and public health implications"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2333-7584","authenticated-orcid":true,"given":"Emmanuel O.","family":"Njoga","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanley U.","family":"Ilo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Obichukwu C.","family":"Nwobi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Onyinye S.","family":"Onwumere-Idolor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Festus E.","family":"Ajibo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chinwe E.","family":"Okoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ishmael F.","family":"Jaja","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6810-4437","authenticated-orcid":true,"given":"James W.","family":"Oguttu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,3,3]]},"reference":[{"issue":"27","key":"pone.0282418.ref001","article-title":"Lysine Supply Is a Critical Factor in Achieving Sustainable Global Protein Economy","volume":"3","author":"I Leinonen","year":"2019","journal-title":"Front Sustain Food Syst"},{"key":"pone.0282418.ref002","unstructured":"Berkheiser K, Brown MJ. Animal vs Plant Protein\u2014What\u2019s the Difference? Available at: https:\/\/wwwhealthlinecom\/nutrition\/animal-vs-plant-protein Accessed 8 June 2022"},{"issue":"3241","key":"pone.0282418.ref003","article-title":"A System Dynamics Model of Supply-Side Issues Influencing Beef Consumption in Nigeria","volume":"12","author":"KG Odoemena","year":"2020","journal-title":"Sustainability"},{"key":"pone.0282418.ref004","unstructured":"FAO Current worldwide annual meat consumption per capita livestock and fish primary equivalent Available at: http:\/\/faostatfaoorg Accessed 14 June 2022"},{"issue":"1","key":"pone.0282418.ref005","first-page":"21","article-title":"Meat consumption patterns among different income groups in Imo State, Nigeria.","volume":"11","author":"CO Anyiro","year":"2013","journal-title":"J. Agric. Soc. Sci"},{"issue":"4","key":"pone.0282418.ref006","first-page":"557","article-title":"Analysis of Meat Consumption Pattern in Ogbomoso North Local Government Area, Oyo State, Nigeria","volume":"42","author":"OV Arowolo","year":"2021","journal-title":"J Appl Sci Environ Mgt"},{"key":"pone.0282418.ref007","unstructured":"Anonymous Nigeria-Total production of meat Available at: https:\/\/knoemacom\/atlas\/Nigeria\/topics\/Agriculture\/Live-Stock-Production-Production-Quantity\/Total-production-of-meat Accessed May 25 2022"},{"issue":"2","key":"pone.0282418.ref008","first-page":"61","article-title":"Abattoir operations and waste management in Nigeria: a review of challenges and prospects","volume":"7","author":"JA Nwanta","year":"2008","journal-title":"Sokoto J Vet Sci"},{"issue":"1","key":"pone.0282418.ref009","first-page":"56","article-title":"Evaluation of physical facilities and processing operations of major abattoirs in North-western states of Nigeria","volume":"11","author":"MK Lawan","year":"2013","journal-title":"Sokoto J Vet Sci"},{"issue":"137","key":"pone.0282418.ref010","article-title":"Role of slaughter facilities management in zoonoses and safety of meat produced for human consumption in Nigeria: a review","volume":"45","author":"S Ibrahim","year":"2021","journal-title":"Bull Natl Res Cent"},{"issue":"6","key":"pone.0282418.ref011","doi-asserted-by":"crossref","first-page":"105","DOI":"10.31248\/JPHD2020.095","article-title":"A review on the state of abattoirs in Nigeria during COVID-19 pandemic era: Potential threats and public health interventions","volume":"3","author":"KA Olawuyi","year":"2020","journal-title":"Journal of Public Health and Diseases"},{"issue":"4","key":"pone.0282418.ref012","doi-asserted-by":"crossref","first-page":"1914","DOI":"10.4314\/ahs.v21i4.50","article-title":"Hygiene practices in abattoir and slaughter slab determinants and assessment of abattoir and slaughter slab facilities in Abakaliki, Ebonyi State, South-East Nigeria.","volume":"21","author":"AP Agu","year":"2021","journal-title":"African Health Sciences"},{"issue":"1","key":"pone.0282418.ref013","doi-asserted-by":"crossref","DOI":"10.1080\/23311932.2015.1106386","article-title":"Assessment of livestock slaughtered for food and meat inspection issues in selected abattoirs in Benue State, Nigeria","volume":"1","author":"K Shima","year":"2015","journal-title":"Cogent Food & Agriculture"},{"key":"pone.0282418.ref014","doi-asserted-by":"crossref","first-page":"174","DOI":"10.3390\/vetsci1030174","article-title":"Effect of Transportation and Pre-Slaughter Handling on Welfare and Meat Quality of Cattle: Case Study of Kumasi Abattoir Ghana","volume":"1","author":"S Frimpong","year":"2014","journal-title":"Vet Sci"},{"issue":"2","key":"pone.0282418.ref015","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1093\/jas\/skx064","article-title":"Preslaughter handling practices and their effects on animal welfare and pork quality","volume":"96","author":"L. Faucitano","year":"2018","journal-title":"J Anim Sci"},{"key":"pone.0282418.ref016","first-page":"484","article-title":"Effect of pre-slaughter animal handling on carcass and meat quality","volume":"18","author":"F. Adzitey","year":"2011","journal-title":"Int Food Res J"},{"key":"pone.0282418.ref017","doi-asserted-by":"crossref","first-page":"108611","DOI":"10.1016\/j.meatsci.2021.108611","article-title":"Dark-cutting beef: A brief review and an integromics meta-analysis at the proteome level to decipher the underlying pathways","volume":"181","author":"M Gagaoua","year":"2021","journal-title":"Meat Sci"},{"issue":"3","key":"pone.0282418.ref018","doi-asserted-by":"crossref","first-page":"2391","DOI":"10.1111\/1541-4337.12935","article-title":"Biomarkers and biosensors for the diagnosis of noncompliant pH dark cutting beef predisposition and welfare in cattle","volume":"21","author":"JLD Nelis","year":"2022","journal-title":"Compr Rev Food Sci Food Saf"},{"issue":"7","key":"pone.0282418.ref019","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1590\/S1516-35982014000700004","article-title":"Effect of low or high stress in pre-slaughter handling on pig carcass and meat quality","volume":"43","author":"LM Peres","year":"2014","journal-title":"R Bras Zootec"},{"issue":"6","key":"pone.0282418.ref020","doi-asserted-by":"crossref","first-page":"755","DOI":"10.5713\/ajas.16.0037","article-title":"Farm to abattoir conditions animal factors and their subsequent effects on cattle behavioural responses and beef quality\u2014A review","volume":"30","author":"YZ Njisane","year":"2017","journal-title":"Asian-Australasian J Anim Sci"},{"key":"pone.0282418.ref021","doi-asserted-by":"crossref","first-page":"104","DOI":"10.3390\/agriculture11020104","article-title":"Costs and Benefits of Improving Farm Animal Welfare","volume":"11","author":"JN Fernandes","year":"2021","journal-title":"Agriculture"},{"key":"pone.0282418.ref022","unstructured":"Anonymous. Meat quality and animal welfare. Available at https:\/\/wwwthecattlesitecom\/articles\/1454\/meat-quality-and-animal-welfare\/ Accessed May 25 2022"},{"issue":"2","key":"pone.0282418.ref023","first-page":"79","article-title":"The Effects of Handling Transport Slaughter and Chilling on Meat Quality and Yield in Pigs: A Review","volume":"13","author":"PV Tarrant","year":"1989","journal-title":"Irish Journal of Food Science and Technology"},{"key":"pone.0282418.ref024","unstructured":"Gill R. Five freedoms of cattle. Available at: https:\/\/animalsciencetamuedu\/2015\/05\/22\/the-five-freedoms-of-cattle\/ Accessed June 1 2022"},{"key":"pone.0282418.ref025","doi-asserted-by":"crossref","DOI":"10.3390\/ani6030021","article-title":"Updating Animal Welfare Thinking: Moving beyond the \"Five Freedoms\" towards \"A Life Worth Living","volume":"6","author":"DJ Mellor","year":"2016","journal-title":"Animals"},{"issue":"1298","key":"pone.0282418.ref026","article-title":"Slaughter Conditions and Slaughtering of Pregnant Cows in Southeast Nigeria: Implications to Meat Quality Food Safety and Security","volume":"10","author":"UJ Njoga","year":"2021","journal-title":"Foods"},{"issue":"9","key":"pone.0282418.ref027","doi-asserted-by":"crossref","first-page":"2216","DOI":"10.3382\/ps\/pew159","article-title":"Immune responses to improving welfare","volume":"95","author":"LR Berghman","year":"2016","journal-title":"Poultry Science"},{"key":"pone.0282418.ref028","doi-asserted-by":"crossref","first-page":"137","DOI":"10.7120\/09627286.28.2.137","article-title":"Animal welfare as preventative medicine","volume":"28","author":"MS Dawkins","year":"2019","journal-title":"Animal Welfare"},{"issue":"6","key":"pone.0282418.ref029","doi-asserted-by":"crossref","first-page":"867","DOI":"10.5713\/ajas.19.0282","article-title":"An outlook on livestock welfare conditions in African communities\u2014A review","volume":"33","author":"YZ Njisane","year":"2020","journal-title":"Asian-Australian J Anim Sci"},{"key":"pone.0282418.ref030","doi-asserted-by":"crossref","DOI":"10.5772\/intechopen.96742","article-title":"Effects of Pre-Slaughter Stress on Meat Characteristics and Consumer Experience In: Ranabhat C L editor","author":"BI Cappellozza","year":"2021","journal-title":"Meat and Nutrition London: IntechOpen"},{"key":"pone.0282418.ref031","first-page":"109","article-title":"Animal welfare and microbiological safety of poultry meat: Impact of different at-farm animal welfare levels on at-slaughterhouse","author":"L Lannetti","year":"2019","journal-title":"Campylobacter and Salmonella contamination Food control"},{"issue":"1405","key":"pone.0282418.ref032","article-title":"Zoonotic Diseases: Etiology Impact and Control","volume":"8","author":"MT Rahman","year":"2020","journal-title":"Microorganisms"},{"issue":"1","key":"pone.0282418.ref033","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/S2221-1691(11)60190-1","article-title":"A survey of zoonotic diseases in trade cattle slaughtered at Tanga city abattoir: a cause of public health concern","volume":"2","author":"ES Swai","year":"2012","journal-title":"Asian Pacific J Trop Biomed"},{"key":"pone.0282418.ref034","first-page":"4674235","article-title":"Review on Major Food-Borne Zoonotic Bacterial Pathogens","author":"A Engidaw","year":"2020","journal-title":"J Tropical Med."},{"key":"pone.0282418.ref035","unstructured":"Worldometers. Nigerian population in 2022 Available online at https:\/\/wwwworldometersinfo\/world-population\/nigeria-population\/ accessed 02 June 2022"},{"key":"pone.0282418.ref036","volume-title":"Encyclopedia of Autism Spectrum Disorders Springer","author":"A. Holmes","year":"2013"},{"issue":"462","key":"pone.0282418.ref037","article-title":"Diagnostic efficiency of abattoir meat inspection service in Ethiopia to detect carcasses infected with Mycobacterium bovis: Implications for public health","volume":"10","author":"D Biffa","year":"2010","journal-title":"BMC Public Health"},{"issue":"9","key":"pone.0282418.ref038","doi-asserted-by":"crossref","DOI":"10.3390\/ani11092620","article-title":"Evaluation of Postmortem Inspection Procedures to Diagnose Bovine Tuberculosis at Debre Birhan Municipal Abattoir","volume":"11","author":"FT Woldemariyam","year":"2021","journal-title":"Animals"},{"issue":"4","key":"pone.0282418.ref039","doi-asserted-by":"crossref","first-page":"195","DOI":"10.4103\/1117-1936.173959","article-title":"Principles and methods of validity and reliability testing of questionnaires used in social and health science researches","volume":"22","author":"OA Bolarinwa","year":"2015","journal-title":"Niger Postgrad Med J"},{"issue":"2","key":"pone.0282418.ref040","doi-asserted-by":"crossref","first-page":"165","DOI":"10.15171\/jcs.2015.017","article-title":"Design and implementation content validity study: Development of an instrument for measuring patient-centered communication","volume":"4","author":"V Zamanzadeh","year":"2015","journal-title":"J Caring Sci"},{"key":"pone.0282418.ref041","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1002\/hec.3100","article-title":"Test\u2013retest reliability of capability measurement in the UK general population","volume":"24","author":"H Al-Janabi","year":"2015","journal-title":"Health Econ"},{"key":"pone.0282418.ref042","doi-asserted-by":"crossref","first-page":"2191","DOI":"10.1001\/jama.2013.281053","article-title":"World Medical Association Declaration of Helsinki: Ethical principles for medical research involving human subjects","volume":"310","author":"World Medical Association (WMA)","year":"2013","journal-title":"JAMA"},{"issue":"3","key":"pone.0282418.ref043","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1007\/s40572-016-0097-9","article-title":"Farm Animal Welfare and Human Health","volume":"3","author":"AM Goldberg","year":"2016","journal-title":"Curr Envir Health Report"},{"issue":"2","key":"pone.0282418.ref044","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1080\/00130095.2018.1508994","article-title":"Making Value Out of Ethics: The Emerging Economic Geography of Lab-grown Meat and Other Animal-free Food Products","volume":"95","author":"MJ Mouat","year":"2019","journal-title":"Economic Geography"},{"key":"pone.0282418.ref045","article-title":"Animal Welfare in Extensive Production Systems Is Still an Area of Concern","author":"D Temple","year":"2020","journal-title":"Front Sustain Food Syst"},{"issue":"1","key":"pone.0282418.ref046","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1080\/14759551.2018.1513937","article-title":"The animal welfare battle: the production of affected ignorance in the Swedish meat industry debate","volume":"26","author":"B. Schwartz","year":"2020","journal-title":"Culture and Organization"},{"key":"pone.0282418.ref047","doi-asserted-by":"crossref","first-page":"678","DOI":"10.3390\/ani12060678","article-title":"Applied Animal Ethics in Industrial Food Animal Production: Exploring the Role of the Veterinarian","volume":"12","author":"E Hernandez","year":"2022","journal-title":"Animals"},{"issue":"5","key":"pone.0282418.ref048","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.3390\/ani11051225","article-title":"Animal Harms and Food Production: Informing Ethical Choices","volume":"11","author":"JO Hampton","year":"2021","journal-title":"Animals"},{"issue":"3","key":"pone.0282418.ref049","doi-asserted-by":"crossref","DOI":"10.1016\/j.animal.2022.100457","article-title":"Animal board invited review: Animal source foods in healthy sustainable and ethical diets\u2013An argument against drastic limitation of livestock in the food system","volume":"16","author":"F Leroy","year":"2022","journal-title":"Animal"},{"issue":"84","key":"pone.0282418.ref050","article-title":"Understanding the Determination of Meat Quality Using Biochemical Characteristics of the Muscle: Stress at Slaughter and Other Missing Keys","volume":"10","author":"EMC Terlouw","year":"2021","journal-title":"Foods"},{"key":"pone.0282418.ref051","first-page":"274","volume-title":"Chemical and physical characteristics of meat | Water-Holding Capacity Editor(s): Michael Dikeman Carrick Devine Encyclopedia of Meat Sciences","author":"MS Brewer","year":"2014","edition":"2"},{"issue":"3","key":"pone.0282418.ref052","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1111\/1541-4337.12258","article-title":"Causes and Contributing Factors to \"Dark Cutting\" Meat: Current Trends and Future Directions: A Review.","volume":"16","author":"EN Ponnampalam","year":"2017","journal-title":"Compr. Rev. Food Sci. Food Saf"},{"key":"pone.0282418.ref053","article-title":"Review on Beef Cattle Pre-slaughter Stress and Its Effect on Meat and Carcass Quality","volume":"78","author":"AF Birhanu","year":"2020","journal-title":"Advances in Life Science and Technology"},{"issue":"490","key":"pone.0282418.ref054","article-title":"Review on Natural Preservatives for Extending Fish Shelf Life","volume":"8","author":"J Mei","year":"2019","journal-title":"Foods"},{"issue":"3","key":"pone.0282418.ref055","article-title":"Meat Spoilage: A Critical Review of a Neglected Alteration Due to Ropy Slime Producing Bacteria","volume":"14","author":"FI Maria","year":"2015","journal-title":"Italian J Anim Sci"},{"issue":"3","key":"pone.0282418.ref056","doi-asserted-by":"crossref","first-page":"529","DOI":"10.3934\/microbiol.2017.3.529","article-title":"Foodborne pathogens","volume":"3","author":"T. Bintsis","year":"2017","journal-title":"AIMS Microbiol"},{"key":"pone.0282418.ref057","volume-title":"Meat and Nutrition","author":"DA Almashhadany","year":"2021"},{"key":"pone.0282418.ref058","doi-asserted-by":"crossref","first-page":"e202","DOI":"10.1016\/S2542-5196(22)00003-1","article-title":"Associations between ambient temperature and enteric infections by pathogen: a systematic review and meta-analysis","volume":"6","author":"PLC Chua","year":"2022","journal-title":"Lancet Planet Health"},{"issue":"3","key":"pone.0282418.ref059","doi-asserted-by":"crossref","first-page":"242","DOI":"10.3390\/metabo12030242","article-title":"Metabolites Analysis on Water-Holding Capacity in Beef Longissimus lumborum Muscle during Postmortem Aging","volume":"12","author":"H Zuo","year":"2022","journal-title":"Metabolites"},{"issue":"252","key":"pone.0282418.ref060","article-title":"Effects of Stocking and Transport Conditions on Physicochemical Properties of Meat and Acute-Phase Proteins in Cattle.","volume":"10","author":"AA Abubakar","year":"2021","journal-title":"Foods"},{"issue":"1","key":"pone.0282418.ref061","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.meatsci.2005.04.022","article-title":"Mechanisms of water-holding capacity of meat: The role of postmortem biochemical and structural changes","volume":"71","author":"E Huff-Lonergan","year":"2005","journal-title":"Meat Sci"},{"key":"pone.0282418.ref062","first-page":"147","article-title":"Fresh meat water-holding capacity Editor(s): Joseph P Kerry David Ledward In Woodhead Publishing Series in Food Science Technology and Nutrition Improving the Sensory and Nutritional Quality of Fresh Meat Woodhead Publishing","author":"E. Huff-Lonergan","year":"2009"},{"issue":"612","key":"pone.0282418.ref063","article-title":"The Impact of Pre-Slaughter Stress on Beef Eating Quality","volume":"9","author":"KMW Loudon","year":"2019","journal-title":"Animals"},{"key":"pone.0282418.ref064","article-title":"Meat quality: Impact of various pre-slaughter conditions","author":"N Karabasil","year":"2019","journal-title":"Earth and Environmental Science"},{"issue":"12","key":"pone.0282418.ref065","doi-asserted-by":"crossref","first-page":"1762","DOI":"10.5713\/ajas.2013.13141","article-title":"The Effects of Pre-slaughter Stress and Season on the Activity of Plasma Creatine Kinase and Mutton Quality from Different Sheep Breeds Slaughtered at a Smallholder Abattoir","volume":"26","author":"AY Chulayo","year":"2013","journal-title":"Asian-Australasian J Anim Sci"},{"issue":"2","key":"pone.0282418.ref066","first-page":"308","volume":"18","author":"T Xing","year":"2018","journal-title":"Stress Effects on Meat Quality: A Mechanistic Perspective Compr Rev Food Sci Saf"},{"issue":"10","key":"pone.0282418.ref067","first-page":"1656","article-title":"Effect of stress during slaughter on carcass characteristics and meat quality in tropical beef cattle","volume":"33","author":"A Carrasco-Garc\u00eda","year":"2020","journal-title":"Anim Biosci"},{"issue":"1","key":"pone.0282418.ref068","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1002\/vms3.2","article-title":"Prevalence of carcass bruises as an indicator of welfare in beef cattle and the relation to the economic impact","volume":"1","author":"SM Huertas","year":"2015","journal-title":"Vet Med Sci"},{"key":"pone.0282418.ref069","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/s11250-016-1173-8","article-title":"Bruises in beef cattle at slaughter in Mexico: implications on quality safety and shelf life of the meat","volume":"49","author":"RG Cruz-Monterrosa","year":"2017","journal-title":"Trop Anim Health Prod"},{"issue":"1","key":"pone.0282418.ref070","doi-asserted-by":"crossref","first-page":"16","DOI":"10.5851\/kosfa.2020.e52","article-title":"Meat Value Chain Losses in Iran","volume":"41","author":"V Ranaei","year":"2021","journal-title":"Food Sci. Anim Res"},{"issue":"27","key":"pone.0282418.ref071","first-page":"116","article-title":"Effect of Stress on Animal Health: A Review","volume":"4","author":"A Asres","year":"2014","journal-title":"J Biology Agric Healthcare"},{"issue":"3","key":"pone.0282418.ref072","first-page":"231","article-title":"Sanitary conditions of abattoirs in Ibadan Southwest Nigeria","volume":"43","author":"EO Oloruntoba","year":"2014","journal-title":"Afr J Med Med Sci"},{"key":"pone.0282418.ref073","first-page":"87","article-title":"Predisposing factors associated with diseases in animals in Nigeria and possible botanical immunostimulants and immunomodulators: a review","volume":"16","author":"KT Biobaku","year":"2018","journal-title":"BJVM"},{"key":"pone.0282418.ref074","doi-asserted-by":"crossref","first-page":"116","DOI":"10.3389\/fvets.2020.00116","article-title":"Heat stress impacts immune status in cows across the life cycle","volume":"7","author":"GE Dahl","year":"2020","journal-title":"Front Vet Sci"},{"issue":"1","key":"pone.0282418.ref075","doi-asserted-by":"crossref","DOI":"10.1093\/conphys\/coz093","article-title":"Chronic captivity stress in wild animals is highly species-specific","volume":"7","author":"CP Fischer","year":"2019","journal-title":"Conserv Physiol"},{"key":"pone.0282418.ref076","unstructured":"European Food Safety Authority (EFSA) Animal welfare Available at: https:\/\/wwwefsaeuropaeu\/en\/topics\/topic\/animal-welfare Accessed June 6 2022"},{"key":"pone.0282418.ref077","volume-title":"Holland-Frei Cancer Medicine","author":"LI McKay","year":"2003","edition":"6"},{"key":"pone.0282418.ref078","volume-title":"Pharmacokinetics and Adverse Effects of Drugs\u2014Mechanisms and Risks Factors:","author":"IS Alan","year":"2017"},{"key":"pone.0282418.ref079","first-page":"277","article-title":"The Cortisol Steroid Levels as a determinant of Health Status in Animals","volume":"10","author":"A Isaac","year":"2017","journal-title":"J Proteomics Bioinform"},{"key":"pone.0282418.ref080","volume-title":"Endotext South Dartmouth (MA","author":"NC Nicolaides","year":"2000"},{"issue":"1","key":"pone.0282418.ref081","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/1710-1492-4-1-2","article-title":"Enhancing versus Suppressive Effects of Stress on Immune Function: Implications for Immunoprotection versus Immunopathology","volume":"4","author":"FS Dhabhar","year":"2008","journal-title":"Allergy Asthma Clin Immunol"},{"issue":"1","key":"pone.0282418.ref082","first-page":"10","article-title":"The effect of stress on the defense systems","volume":"3","author":"D Drago\u015f","year":"2010","journal-title":"J Med Life"},{"key":"pone.0282418.ref083","doi-asserted-by":"crossref","first-page":"245","DOI":"10.3389\/fimmu.2019.00245","article-title":"Editorial: Stress and Immunity","volume":"10","author":"YS Bae","year":"2019","journal-title":"Front 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Garc\u00eda","year":"2003","journal-title":"Lancet"},{"key":"pone.0282418.ref094","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s00436-016-5278-9","article-title":"Neurocysticercosis: a review on status in India management and current therapeutic interventions","volume":"116","author":"R Ahmad","year":"2017","journal-title":"Parasitol Res"},{"key":"pone.0282418.ref095","unstructured":"WHO 2022 Taeniasis\/cysticercosis Available at: https:\/\/wwwwhoint\/news-room\/fact-sheets\/detail\/taeniasis-cysticercosis accessed on 7 June 2022"},{"issue":"2","key":"pone.0282418.ref096","doi-asserted-by":"crossref","first-page":"36","DOI":"10.5897\/JVMAH2019.0756","article-title":"Assessment of operational facilities and sanitary practices in Zangon Shanu abattoir Sabon Gari Local Government Area Kaduna State Nigeria","volume":"12","author":"AU Gali","year":"2020","journal-title":"Journal of Veterinary Medicine and Animal 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Trop"},{"key":"pone.0282418.ref100","doi-asserted-by":"crossref","first-page":"147","DOI":"10.3923\/ajava.2015.147.160","article-title":"Causes of Beef Carcass and Organ Condemnations in Ethiopia","volume":"10","author":"YY Mummed","year":"2015","journal-title":"Asian Journal of Animal and Veterinary Advances"},{"issue":"8","key":"pone.0282418.ref101","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1179\/136485910X12851868780261","article-title":"The abattoir condemnation of meat because of parasitic infection and its economic importance: results of a retrospective study in north-eastern Iran","volume":"104","author":"H Borji","year":"2010","journal-title":"Ann Trop Med Parasitol"},{"issue":"198","key":"pone.0282418.ref102","article-title":"Prevalence causes and economic losses resulting from condemnations in the slaughterhouses\/slabs in Eastern and North-eastern zones of Tanzania","volume":"28","author":"C Kiswaga","year":"2022","journal-title":"Livestock Research for Rural Development"},{"key":"pone.0282418.ref103","first-page":"163","article-title":"Causes patterns and economic implications of carcass condemnation of cattle slaughtered at Oshana region North of Namibia based on post-mortem inspection","volume":"7","author":"P Mbiri","year":"2020","journal-title":"Journal of Food Quality and Hazards Control"},{"issue":"2","key":"pone.0282418.ref104","doi-asserted-by":"crossref","first-page":"27","DOI":"10.5897\/JVMAH2019.0779","article-title":"Causes of organ condemnations in cattle at slaughter and associated financial losses in Siaya County Kenya","volume":"12","author":"AM Achollah","year":"2020","journal-title":"Journal of Veterinary Medicine and Animal Health"},{"issue":"3","key":"pone.0282418.ref105","doi-asserted-by":"crossref","first-page":"406","DOI":"10.5713\/ajas.2013.13437","article-title":"Bleeding Efficiency and Meat Oxidative Stability and Microbiological Quality of New Zealand White Rabbits Subjected to Halal Slaughter without Stunning and Gas Stun-killing","volume":"27","author":"K Nakyinsige","year":"2014","journal-title":"Asian-Australas J Anim Sci"},{"key":"pone.0282418.ref106","doi-asserted-by":"crossref","first-page":"530","DOI":"10.3390\/ani9080530","article-title":"Halal criteria versus conventional slaughter technology","volume":"9","author":"F Abdullah","year":"2019","journal-title":"Animals"},{"issue":"5","key":"pone.0282418.ref107","first-page":"524","article-title":"Effect of slaughter technique on bleed-out blood in the trachea and blood splash in the lungs of cattle","volume":"42","author":"B Agbeniga","year":"2012","journal-title":"S Afr J Anim Sci"},{"key":"pone.0282418.ref108","doi-asserted-by":"crossref","first-page":"176","DOI":"10.3390\/foods9020176","article-title":"Sensory and Physicochemical Analysis of Meat from Bovine Breeds in Different Livestock Production Systems Pre-Slaughter Handling Conditions and Ageing Time","volume":"9","author":"M L\u00f3pez-Pedrouso","year":"2020","journal-title":"Foods"},{"issue":"4","key":"pone.0282418.ref109","doi-asserted-by":"crossref","first-page":"e0152661","DOI":"10.1371\/journal.pone.0152661","article-title":"Bleeding Efficiency, Microbiological Quality and Oxidative Stability of Meat from Goats Subjected to Slaughter without Stunning in Comparison with Different Methods of Pre-Slaughter Electrical Stunning","volume":"11","author":"AB Sabow","year":"2016","journal-title":"PloS One"},{"issue":"104904","key":"pone.0282418.ref110","article-title":"Slaughtering of heifers in a local or an industrial abattoir: Animal welfare and meat quality consequences","volume":"259","author":"P Guarnido-L\u00f3pez","year":"2022","journal-title":"Livestock Science"},{"key":"pone.0282418.ref111","first-page":"101","article-title":"Major Causes Of Meat Spoilage and Preservation Techniques: A Review","volume":"41","author":"M. 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