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In the Pacific Arctic Region spanning the Bering, Chukchi, and Beaufort seas, DA is recognized as an emerging human and ecosystem health threat, yet little is known about the composition and distribution of <jats:italic>Pseudo-nitzschia<\/jats:italic> species in these waters. This investigation characterized <jats:italic>Pseudo-nitzschia<\/jats:italic> assemblages in samples collected in 2018 during summer (August) and fall (October-November) surveys as part of the Distributed Biological Observatory and Arctic Observing Network, encompassing a broad geographic range (57.8\u00b0 to 73.0\u00b0N, -138.9\u00b0 to -169.9\u00b0W) and spanning temperature (-1.79 to 11.7\u00b0C) and salinity (22.9 to 32.9) gradients associated with distinct water masses. Species were identified using a genus-specific Automated Ribosomal Intergenic Spacer Analysis (ARISA). Seventeen amplicons were observed; seven corresponded to temperate, sub-polar, or polar <jats:italic>Pseudo-nitzschia<\/jats:italic> species based on parallel sequencing efforts (<jats:italic>P<\/jats:italic>. <jats:italic>arctica<\/jats:italic>, <jats:italic>P<\/jats:italic>. <jats:italic>delicatissima<\/jats:italic>, <jats:italic>P<\/jats:italic>. <jats:italic>granii<\/jats:italic>, <jats:italic>P<\/jats:italic>. <jats:italic>obtusa<\/jats:italic>, <jats:italic>P<\/jats:italic>. <jats:italic>pungens<\/jats:italic>, and two genotypes of <jats:italic>P<\/jats:italic>. <jats:italic>seriata<\/jats:italic>), and one represented <jats:italic>Fragilariopsis oceanica<\/jats:italic>. During summer, particulate DA (pDA; 4.0 to 130.0 ng L<jats:sup>-1<\/jats:sup>) was observed in the Bering Strait and Chukchi Sea where <jats:italic>P<\/jats:italic>. <jats:italic>obtusa<\/jats:italic> was prevalent. In fall, pDA (3.3 to 111.8 ng L<jats:sup>-1<\/jats:sup>) occurred along the Beaufort Sea shelf coincident with one <jats:italic>P<\/jats:italic>. <jats:italic>seriata<\/jats:italic> genotype, and south of the Bering Strait in association with the other <jats:italic>P<\/jats:italic>. <jats:italic>seriata<\/jats:italic> genotype. Taxa were correlated with latitude, longitude, temperature, salinity, pDA, and\/or chlorophyll <jats:italic>a<\/jats:italic>, and each had a distinct distribution pattern. The observation of DA in association with different species, seasons, geographic regions, and water masses underscores the significant risk of Amnesic Shellfish Poisoning (ASP) and DA-poisoning in Alaska waters.<\/jats:p>","DOI":"10.1371\/journal.pone.0282794","type":"journal-article","created":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T17:28:54Z","timestamp":1679506134000},"page":"e0282794","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":18,"title":["Spatiotemporal transitions in Pseudo-nitzschia species assemblages and domoic acid along the Alaska coast"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0267-0345","authenticated-orcid":true,"given":"Katherine A.","family":"Hubbard","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria C\u00e9lia","family":"Villac","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Chadwick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra A.","family":"DeSmidt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leanne","family":"Flewelling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"April","family":"Granholm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Molly","family":"Joseph","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taylor","family":"Wood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evangeline","family":"Fachon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael L.","family":"Brosnahan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mindy","family":"Richlen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mrunmayee","family":"Pathare","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dean","family":"Stockwell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peigen","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9e N.","family":"Bouchard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Pickart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald M.","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,3,22]]},"reference":[{"issue":"8","key":"pone.0282794.ref001","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1093\/oxfordjournals.aje.a117499","article-title":"Paralytic shellfish poisoning in Alaska: A 20-year retrospective analysis","volume":"141","author":"B Gessner","year":"1995","journal-title":"American Journal of Epidemiology"},{"key":"pone.0282794.ref002","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.hal.2012.06.009","article-title":"Harmful algal blooms along the North American west coast region: History, trends, causes, and impacts","volume":"19","author":"A Lewitus","year":"2012","journal-title":"Harmful Algae"},{"key":"pone.0282794.ref003","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.hal.2016.01.007","article-title":"Prevalence of algal toxins in Alaskan marine mammals foraging in a changing Arctic and subarctic environment","volume":"55","author":"K Lefebvre","year":"2016","journal-title":"Harmful Algae"},{"key":"pone.0282794.ref004","unstructured":"Anderson D, Richlen M, Lefebvre K. 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