{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T03:15:00Z","timestamp":1784603700219,"version":"3.55.0"},"reference-count":34,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T00:00:00Z","timestamp":1681430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Vaginal colonization with lactobacilli has been linked to the health of the lower urinary tract in women. There is growing evidence that the bladder has its microbiome related closely to the vagina. In this study, we compared the three common vaginal <jats:italic>Lactobacillus<\/jats:italic> species (<jats:italic>L<\/jats:italic>. <jats:italic>jensenii<\/jats:italic>, <jats:italic>L<\/jats:italic>. <jats:italic>iners<\/jats:italic> and <jats:italic>L<\/jats:italic>. <jats:italic>crispatus<\/jats:italic>) in vaginal and urine samples to identify factors that influence urinary detection and the quantity of <jats:italic>Lactobacillus<\/jats:italic>. We used quantitative real-time PCR (qPCR) assays to measure the concentration of <jats:italic>Lactobacillus jensenii<\/jats:italic>, <jats:italic>L<\/jats:italic>. <jats:italic>iners<\/jats:italic> and <jats:italic>L<\/jats:italic>. <jats:italic>crispatus<\/jats:italic> in paired vaginal swabs and clean-catch urine samples from pre-and post-menopausal women. We compared demographic variables and vaginal <jats:italic>Lactobacillus<\/jats:italic> quantity between women with vaginal detection of at least one of the three species, detection in both vagina and urine, or urine only. We performed Spearman correlation between vaginal and urinary quantities of each species. We used multivariable logistic regression models to determine predictors of detectable <jats:italic>Lactobacillus<\/jats:italic> species in both samples (vs. vagina only or urine only). Models were adjusted for variables selected a priori: age, BMI, condom use, and recent sexual activity. Ninety-three paired vaginal fluid, and urine samples were included in the final analysis. 44 (47%) had no detectable <jats:italic>Lactobacillus<\/jats:italic> species in their urine samples, and 49 (53%) had at least one of the three <jats:italic>Lactobacillus<\/jats:italic> species (<jats:italic>L<\/jats:italic>. <jats:italic>jensenii<\/jats:italic>, <jats:italic>L<\/jats:italic>. <jats:italic>iners<\/jats:italic> and <jats:italic>L<\/jats:italic>. <jats:italic>crispatus<\/jats:italic>) detected in urine. Most women were white (91.4%), with a mean age of 39.8 \u00b113.8 years. The two groups were similar in demographics, gynecologic history, sexual history, recent use of antibiotics or probiotics within 7 days of sample collection, Nugent scores, and urine-specific gravity. Among the three <jats:italic>Lactobacillus<\/jats:italic> species, <jats:italic>L<\/jats:italic>. <jats:italic>jensenii<\/jats:italic> was more commonly detected in urine than the other two. For all three species, detection in the urine sample alone was infrequent. The concentrations of all three species were higher in vaginal samples than in urine samples. For all three <jats:italic>Lactobacillus<\/jats:italic> spp., vaginal abundance was associated with the urinary abundance of the same species even after adjusting for the Nugent score. In Spearman correlation analysis, urinary and vaginal <jats:italic>Lactobacillus<\/jats:italic> concentrations were positively correlated within the same species, with the most significant correlation coefficient for <jats:italic>L<\/jats:italic>. <jats:italic>jensenii<\/jats:italic> (R = 0.43, p&lt;0.0001). Vaginal quantities were positively correlated between the three species, as were urinary quantities to a lesser extent. There was no meaningful correlation between the urinary quantity of one <jats:italic>Lactobacillus<\/jats:italic> sp. and the vaginal quantity of another species. In summary, the vaginal quantity of <jats:italic>Lactobacillus<\/jats:italic> was the most significant predictor of concurrent detection of the same species in the bladder, confirming the close relationship between these environments. Strategies to promote vaginal <jats:italic>Lactobacillus<\/jats:italic> colonization may also bring urinary colonization and the health of the lower urinary tract.<\/jats:p>","DOI":"10.1371\/journal.pone.0283215","type":"journal-article","created":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T17:43:11Z","timestamp":1681494191000},"page":"e0283215","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Vaginal and urinary evaluation of lactobacilli quantification by qPCR: Identifying factors that influence urinary detection and the quantity of Lactobacillus"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8407-7421","authenticated-orcid":true,"given":"Youngwu","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnes","family":"Bergerat-Thompson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9924-2349","authenticated-orcid":true,"given":"Caroline","family":"Mitchell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,4,14]]},"reference":[{"key":"pone.0283215.ref001","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1128\/JCM.02876-13","article-title":"Urine is not sterile: use of enhanced urine culture techniques to detect resident bacterial flora in the adult female bladder","volume":"52","author":"EE Hilt","year":"2014","journal-title":"J Clin Microbiol"},{"key":"pone.0283215.ref002","doi-asserted-by":"crossref","first-page":"41","DOI":"10.3389\/fcimb.2013.00041","article-title":"The human urinary microbiome; bacterial DNA in voided urine of asymptomatic adults","volume":"3","author":"DA Lewis","year":"2013","journal-title":"Front Cell Infect Microbiol"},{"key":"pone.0283215.ref003","doi-asserted-by":"crossref","first-page":"e01283","DOI":"10.1128\/mBio.01283-14","article-title":"The female urinary microbiome: a comparison of women with and without urgency urinary incontinence.","volume":"5","author":"MM Pearce","year":"2014","journal-title":"MBio"},{"key":"pone.0283215.ref004","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.ejogrb.2017.04.040","article-title":"A case controlled study examining the bladder microbiome in women with Overactive Bladder (OAB) and healthy controls.","volume":"214","author":"N Curtiss","year":"2017","journal-title":"Eur J Obstet Gynecol 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Gynecol"},{"key":"pone.0283215.ref008","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1007\/s00192-015-2847-x","article-title":"Incontinence medication response relates to the female urinary microbiota.","volume":"27","author":"KJ Thomas-White","year":"2016","journal-title":"Int Urogynecology J"},{"key":"pone.0283215.ref009","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1097\/AOG.0000000000001892","article-title":"Urinary Microbiome and Cytokine Levels in Women With Interstitial Cystitis.","volume":"129","author":"MG Abernethy","year":"2017","journal-title":"Obstet Gynecol"},{"key":"pone.0283215.ref010","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1007\/s00192-018-3767-3","article-title":"Urinary microbes and postoperative urinary tract infection risk in urogynecologic surgical patients.","volume":"29","author":"KJ Thomas-White","year":"2018","journal-title":"Int Urogynecology 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Infect Microbiol"},{"key":"pone.0283215.ref014","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1128\/jcm.29.2.297-301.1991","article-title":"Reliability of diagnosing bacterial vaginosis is improved by a standardized method of gram stain interpretation","volume":"29","author":"RP Nugent","year":"1991","journal-title":"J Clin Microbiol"},{"key":"pone.0283215.ref015","doi-asserted-by":"crossref","first-page":"1899","DOI":"10.1056\/NEJMoa043802","article-title":"Molecular identification of bacteria associated with bacterial vaginosis","volume":"353","author":"DN Fredricks","year":"2005","journal-title":"N Engl J Med"},{"key":"pone.0283215.ref016","doi-asserted-by":"crossref","first-page":"e0222962","DOI":"10.1371\/journal.pone.0222962","article-title":"Optimizing bacterial DNA extraction in urine","volume":"14","author":"MM Munch","year":"2019","journal-title":"PloS 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Physiol"},{"key":"pone.0283215.ref020","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/j.chom.2020.06.022","article-title":"The Urinary Tract Microbiome in Older Women Exhibits Host Genetic and Environmental Influences","volume":"28","author":"AS Adebayo","year":"2020","journal-title":"Cell Host Microbe"},{"key":"pone.0283215.ref021","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.chom.2018.01.013","article-title":"Cervicovaginal Microbiota and Reproductive Health: The Virtue of Simplicity","volume":"23","author":"MN Anahtar","year":"2018","journal-title":"Cell Host Microbe"},{"key":"pone.0283215.ref022","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1097\/OLQ.0000000000001143","article-title":"Vaginal Microbiota and Mucosal Immune Markers in Women With Vulvovaginal Discomfort","volume":"47","author":"CM Mitchell","year":"2020","journal-title":"Sex Transm Dis"},{"key":"pone.0283215.ref023","doi-asserted-by":"crossref","first-page":"863208","DOI":"10.3389\/fcimb.2022.863208","article-title":"Clustering Analysis of the Multi-Microbial Consortium by Lactobacillus Species Against Vaginal Dysbiosis Among Ecuadorian Women.","volume":"12","author":"D Pacha-Herrera","year":"2022","journal-title":"Front Cell Infect Microbiol"},{"key":"pone.0283215.ref024","doi-asserted-by":"crossref","first-page":"E130","DOI":"10.3390\/microorganisms8010130","article-title":"Warding Off Recurrent Yeast and Bacterial Vaginal Infections: Lactoferrin and Lactobacilli","volume":"8","author":"F Superti","year":"2020","journal-title":"Microorganisms"},{"key":"pone.0283215.ref025","doi-asserted-by":"crossref","first-page":"4610","DOI":"10.1172\/JCI57172","article-title":"Vaginal microbiome and sexually transmitted infections: an epidemiologic perspective","volume":"121","author":"RM Brotman","year":"2011","journal-title":"J Clin Invest"},{"key":"pone.0283215.ref026","doi-asserted-by":"crossref","first-page":"133","DOI":"10.3389\/fcimb.2019.00133","article-title":"The Current Evidence on the Association Between the Urinary Microbiome and Urinary Incontinence in Women.","volume":"9","author":"Y Govender","year":"2019","journal-title":"Front Cell Infect Microbiol"},{"key":"pone.0283215.ref027","first-page":"4","article-title":"The Vaginal Microbiota and Urinary Tract Infection.","author":"AE Stapleton","year":"2016","journal-title":"Microbiol Spectr"},{"key":"pone.0283215.ref028","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1111\/1471-0528.15920","article-title":"The urobiome of continent adult women: a cross-sectional study.","volume":"127","author":"TK Price","year":"2020","journal-title":"BJOG Int J Obstet Gynaecol"},{"key":"pone.0283215.ref029","doi-asserted-by":"crossref","first-page":"24380","DOI":"10.1038\/srep24380","article-title":"Effects of low dose estrogen therapy on the vaginal microbiomes of women with atrophic vaginitis.","volume":"6","author":"J Shen","year":"2016","journal-title":"Sci Rep"},{"key":"pone.0283215.ref030","doi-asserted-by":"crossref","first-page":"732423","DOI":"10.3389\/fcimb.2021.732423","article-title":"The Effect of Exogenous Sex Steroids on the Vaginal Microbiota: A Systematic Review.","volume":"11","author":"LK Ratten","year":"2021","journal-title":"Front Cell Infect Microbiol"},{"key":"pone.0283215.ref031","doi-asserted-by":"crossref","first-page":"590877","DOI":"10.3389\/fmicb.2020.590877","article-title":"Effect of Menopausal Hormone Therapy on the Vaginal Microbiota and Genitourinary Syndrome of Menopause in Chinese Menopausal Women.","volume":"11","author":"L Geng","year":"2020","journal-title":"Front Microbiol"},{"key":"pone.0283215.ref032","first-page":"CD005131","article-title":"Oestrogens for preventing recurrent urinary tract infection in postmenopausal women.","author":"C Perrotta","year":"2008","journal-title":"Cochrane Database Syst Rev"},{"key":"pone.0283215.ref033","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1056\/NEJM199309093291102","article-title":"A controlled trial of intravaginal estriol in postmenopausal women with recurrent urinary tract infections","volume":"329","author":"R Raz","year":"1993","journal-title":"N Engl J Med"},{"key":"pone.0283215.ref034","doi-asserted-by":"crossref","first-page":"1981","DOI":"10.1016\/j.juro.2013.04.142","article-title":"Nonantibiotic prophylaxis for recurrent urinary tract infections: a systematic review and meta-analysis of randomized controlled trials.","volume":"190","author":"M a. J Beerepoot","year":"2013","journal-title":"J Urol"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0283215","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T17:43:31Z","timestamp":1681494211000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0283215"}},"subtitle":[],"editor":[{"given":"Brenda A.","family":"Wilson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,4,14]]},"references-count":34,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,4,14]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0283215","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,14]]}}}